Abstract
Urine is often sampled from patients participating in clinical and metabolomic studies. Biological homeostasis occurs in humans, but little is known about the variability of metabolites found in urine. It is important to define the inter- and intra-individual metabolite variance within a normal population before scientific or clinical conclusions are made regarding different pathophysiologies. This study investigates the variability of selected urine metabolites in a group of 60 healthy men and women over a period of 30 days. To monitor individual variation, 6 women from the normal population were randomly selected and followed for 30 days. To determine the influence of extraneous environmental factors urine was collected from 25 guinea pigs with similar genetics, diet, and living environment. For both studies, 24 metabolites were identified and quantified using high-resolution 1H nuclear magnetic resonance spectroscopy (NMR). The data demonstrated large inter and intra-individual variation in metabolite concentrations in both normal human and control animal populations. A defined normal baseline is essential before any conclusions may be drawn regarding changes in urine metabolite concentrations.
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Acknowledgments
The authors would like to thank the volunteers for their participation in the study, Bruce Lix for his assistance with organization, Shana Regush for her assistance with sample preparation and data acquisition, Carol Mangan for her logistical management of the study, Dr. Edward Akhmetshin for his expertise in statistical analysis, and Ryan McKay for his thorough reviews of the manuscript. This research was supported by the Canadian Institutes of Health Research (CIHR), Genome Prairie, and Genome Canada, and by an establishment grant from the Alberta Heritage Foundation for Medical Research (AHFMR) to T. Marrie. We would like to thank the Canadian National High Field NMR Centre (NANUC) for their assistance and use of the facilities. CIHR, the Natural Science and Engineering Research Council of Canada and the University of Alberta fund the operation of NANUC. Drs. Rowe and Sykes are supported by Canada Research Chairs. Dr. Adamko is supported by AHFMR as a Clinical Investigator. Magnetic Resonance Diagnostic Centre (MRDC) is supported by establishment grants from the Alberta Science and Research Authority (ASRA), and Western Economic Development (WED). All of the authors are members of the Medical Resonance Diagnostics Centre (MRDC) at the University of Alberta, Edmonton, Alberta, Canada.
Support: Canadian Institutes of Health Research (CIHR), Genome Prairie, Genome Canada, an establishment grant from the Alberta Heritage Foundation for Medical Research (AHFMR), the Canadian National High Field NMR Centre (NANUC), the Natural Science and Engineering Research Council of Canada (NSERC), the University of Alberta fund, the Magnetic Resonance Diagnostic Centre (MRDC), the Alberta Science and Research Authority (ASRA), and Western Economic Development (WED).
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Saude, E.J., Adamko, D., Rowe, B.H. et al. Variation of metabolites in normal human urine. Metabolomics 3, 439–451 (2007). https://doi.org/10.1007/s11306-007-0091-1
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DOI: https://doi.org/10.1007/s11306-007-0091-1