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NTPDase2 and the P2Y1 receptor are not required for mammalian eye formation

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Abstract

Eye formation in vertebrates is controlled by a conserved pattern of molecular networks. Homeobox transcription factors are crucially involved in the establishment and maintenance of the retina. A previous study of Massé et al. (Nature, 449: 1058–62, 2007) using morpholino knockdown identified the ectonucleotidase NTPDase2 and the P2Y1 receptor as essential elements for eye formation in embryos of the clawed frog Xenopus laevis. In order to investigate whether a similarly essential mechanism would be active in mammalian eye development, we analyzed mice KO for Entpd2 or P2ry1 as well as double KO for Entpd2/P2ry1. These mice developed normal eyes. In order to identify potential deficits in the molecular identity or in the arrangement of the cellular elements of the retina, we performed an immunohistological analysis using a variety of retinal markers. The analysis of single and double KO mice demonstrated that NTPDase2 and P2Y1 receptors are not required for murine eye formation, as previously shown for eye development in Xenopus laevis.

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Acknowledgments

This work was supported by grants from the Cluster of Excellence EXC 115 and Gutenberg Research College (GCR) Mainz University (to A A-P) and from NIH (R21 CA164970/NCI and HL R01 094400/NHLBI) (to SC R).

Conflict of interest

The authors indicate no potential conflicts of interest.

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Authors and Affiliations

  1. Institute of Cell Biology and Neuroscience, Goethe-University, Frankfurt am Main, Max-von-Laue-Str. 13, Germany

    Kristine Gampe, Amparo Acker-Palmer & Herbert Zimmermann

  2. Max-Planck-Institute for Brain Research, Frankfurt am Main, Germany

    Silke Haverkamp & Laura Hüser

  3. Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA

    Simon C. Robson

  4. EFS-Alsace, INSERM, Université de Strasbourg, Strasbourg, France

    Christian Gachet

  5. Focus Program Translational Neurosciences (FTN), University of Mainz, Mainz, Germany

    Amparo Acker-Palmer

Authors
  1. Kristine Gampe
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  2. Silke Haverkamp
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  3. Simon C. Robson
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  4. Christian Gachet
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  5. Laura Hüser
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  6. Amparo Acker-Palmer
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  7. Herbert Zimmermann
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Correspondence to Kristine Gampe.

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Gampe, K., Haverkamp, S., Robson, S.C. et al. NTPDase2 and the P2Y1 receptor are not required for mammalian eye formation. Purinergic Signalling 11, 155–160 (2015). https://doi.org/10.1007/s11302-014-9440-5

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  • DOI: https://doi.org/10.1007/s11302-014-9440-5

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