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Changes in protein profile of Platycladus orientalis (L.) roots and leaves in response to drought stress

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Abstract

Platycladus orientalis (L.) Franco is widely used for afforestation in arid and semi-arid areas due to its high drought tolerance. To better understand the mechanism involved in drought tolerance in this important tree, responses to drought stress have been studied in 1-year-old P. orientalis via water withholding. Several physiological parameters were evaluated in four drought-treated groups. The root and leaf proteomes of two-dimensional electrophoresis (2-DE) gels were obtained, and a total of 162 proteins with significant quantitative variations and 1.5-fold differences in proteins were selected and identified by matrix-assisted laser desorption/ionization-time-of-flight/time-of-flight (MALDI-TOF/TOF) tandem mass spectrometry (MS/MS). The majority of identified proteins were classified into functional categories, including stress response/defense, carbohydrate metabolic process, nitrogen metabolism, proteolysis, and photosynthesis. The proteome results revealed that a series of strategies were employed to survive in the drought environment, such as the maintenance of protein stability, activation, and folding; reactive oxygen species (ROS) detoxification; the regulation of cell osmotic conditions and cell wall integrity; energy metabolism; and stabilization of the cell skeleton. One of the most prominent findings in this study was the number of bark protein-like proteins and heat shock proteins detected in both root and leaf tissues in drought stress conditions. In addition, 12 differentially expressed proteins were selected for quantitative reverse transcription PCR analysis; not all of the protein expression levels were consistent with the mRNA expression levels. Our data provide a comprehensive picture of the root and leaf responses under varying watering regimes, which could be beneficial for further research and for understanding highly complex drought stress responses.

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Acknowledgements

This work was financially supported by the National Forestry Industry Research Special Funds for Public Welfare Projects (China) (201404302).

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Authors and Affiliations

  1. College of Forestry, Northwest A&F University, Yangling, 712100, Shaanxi, People’s Republic of China

    Sheng Zhang, Lingling Zhang, Kaikai Zhou, Yiming Li & Zhong Zhao

  2. Key Comprehensive Laboratory of Forestry, Yangling, 712100, Shaanxi, People’s Republic of China

    Sheng Zhang, Kaikai Zhou, Yiming Li & Zhong Zhao

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  1. Sheng Zhang
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  2. Lingling Zhang
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  3. Kaikai Zhou
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  4. Yiming Li
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  5. Zhong Zhao
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Contributions

ZZ developed and supervised the work. The preparation of plant material, 2-DE gel analysis, gene-expression analysis, and data analysis were conducted by SZ and LLZ. KKZ and YML helped in the sample collection and qRT-PCR experiment. All authors read and approved the final manuscript.

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Correspondence to Zhong Zhao.

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The authors declare that they have no competing interests.

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Communicated by C. Chen

Data archiving statement

The local database search file (raw data from the Illumina sequencing of P. orientalis) of protein identification has been uploaded in NCBI SRA under accession number (SRX1717969~SRX1717974).

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Zhang, S., Zhang, L., Zhou, K. et al. Changes in protein profile of Platycladus orientalis (L.) roots and leaves in response to drought stress. Tree Genetics & Genomes 13, 76 (2017). https://doi.org/10.1007/s11295-017-1159-3

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