Abstract
Long terminal repeat (LTR) retrotransposons are ubiquitous in the plant kingdom and play an important role in plant genome evolution. Masson pine (Pinus massoniana) is one of the most economically important forest trees. However, the masson pine genome is poorly understood. Conserved domains of reverse transcriptase (RT) genes of Ty1-copia and Ty3-gypsy LTR retrotransposons were amplified from masson pine using degenerate primers. Sequence analysis showed less heterogeneity among Ty1-copia group retrotransposons than among Ty3-gypsy group retrotransposons. Phylogenetic analysis demonstrated that Ty1-copia and Ty3-gypsy RT sequences had considerable homology with other species, indicating that both vertical transmission and horizontal transmission may probably be the source of LTR retrotransposons in masson pine. Southern dot blot hybridization results suggested that both types of LTR retrotransposons are present in the genome of masson pine with high copy number. These results contribute to further understanding about the organization and evolution of the masson pine genome and provide fundamental genetic information for the utilization of retrotransposons in masson pine.
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Acknowledgments
The project is supported by grants from the 863 Program of China (2011AA1002031), Special Core Program of Guizhou Province, P. R. China (20126011–1), and Core Program of Educational Department of Guizhou Province, P. R. China (20090134). We gratefully acknowledge Yang He, Wuhan University, and Guang Qiao, Guizhou University, for critically reading the manuscript. We gratefully acknowledge Mervin Jerry Bartholomew, University of Memphis, for improving the English.
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Fan, F., Wen, X., Ding, G. et al. Isolation, identification, and characterization of genomic LTR retrotransposon sequences from masson pine (Pinus massoniana). Tree Genetics & Genomes 9, 1237–1246 (2013). https://doi.org/10.1007/s11295-013-0631-y
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DOI: https://doi.org/10.1007/s11295-013-0631-y