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Correlations of expression of cell wall biosynthesis genes with variation in biomass composition in shrub willow (Salix spp.) biomass crops

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Abstract

We have measured significant genetically determined variation in biomass composition among breeding populations of shrub willow, a biomass feedstock crop. This project was aimed to ask whether patterns of cell wall gene expression can be correlated with genetic variation in biomass composition at harvest, in order to develop assays of early differences in gene expression as indicators of harvestable biomass chemical composition and potentially reduce the time of selection for new willow genotypes. Previous studies have demonstrated that manipulation of expression of cell wall biosynthetic genes results in altered biomass chemical composition. We analyzed genes encoding enzymes involved in lignin biosynthesis and carbohydrate active enzymes selected based on their functional characterization and conservation in Populus trichocarpa and Arabidopsis thaliana. Fragments of 20 genes were cloned from young stem cDNA of Salix sachalinensis and Salix miyabeana. Expression profiling in willow stem apical tissue and developing stem tissue was performed for each isolated gene using probe-based quantitative real-time PCR. Two willow parental genotypes and six progeny within a hybrid family were selected for analysis, and significant differences in expression among the individuals and between tissue types were observed for most of the genes. Significant correlations between patterns of gene expression and variation in the biomass chemical composition of those genotypes provide insight into the genetic regulation of lignocellulosic deposition in this important bioenergy crop and could be utilized as a tool for early selection of new genotypes.

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Acknowledgments

This work was funded by a grant from the USDA-CSREES McIntire-Stennis Cooperative Forestry Research Program to Dr. Larry B. Smart. Michelle Serapiglia was generously supported by the Josiah Lowe and Hugh Wilcox Scholarship Fund and the Edna Bailey Sussman Foundation. Appreciation is also expressed to Drs. William Powell, Larry Abrahamson, Tim Volk, Ed White, and Bill Winter for their support and advice as collaborators with this research and to Mark Appleby and Ken Burns for the excellent technical support. Special thanks go to Dr. Martin Goffinet for the assistance with histochemical and microscopy analysis of willow stems and to two anonymous reviewers for their insightful suggestions of improvements to this paper.

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  1. Department of Horticulture, Cornell University, New York State Agricultural Experiment Station, 630 West North Street, Geneva, NY, 14456, USA

    Michelle J. Serapiglia, Kimberly D. Cameron & Lawrence B. Smart

  2. Department of Chemistry, State University of New York College of Environmental Science and Forestry, 1 Forestry Drive, Syracuse, NY, 13210, USA

    Arthur J. Stipanovic

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  1. Michelle J. Serapiglia
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  2. Kimberly D. Cameron
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  3. Arthur J. Stipanovic
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  4. Lawrence B. Smart
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Correspondence to Lawrence B. Smart.

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Serapiglia, M.J., Cameron, K.D., Stipanovic, A.J. et al. Correlations of expression of cell wall biosynthesis genes with variation in biomass composition in shrub willow (Salix spp.) biomass crops. Tree Genetics & Genomes 8, 775–788 (2012). https://doi.org/10.1007/s11295-011-0462-7

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