Abstract
Apple (Malus domestica Borkh), a member of the Rosaceae, shows gametophytic self-incompatibility (GSI) controlled by polymorphic S-alleles. Identifying the S-genotypes of apple cultivars can be applied on correct assignment of apple cultivars to cross-compatibility groups, which is important for the efficient production of apple fruit. This study characterized three new S-alleles (designated S 44 , S 45 , and S 46 ) in apple and developed an efficient analysis method that can be used to characterize S-genotypes by utilizing allele-specific polymerase chain reaction rapidly. Nineteen allele-specific primers were selectively designed to identify different alleles. Using this method, S-genotypes of 157 apple cultivars were identified.
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Acknowledgments
This work was supported by a grant from the National Natural Science Foundation of China (No. 30671443). We thank the Fruit Tree Institute of Liaoning Province, China for kindly providing partial plant materials.
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Long, S., Li, M., Han, Z. et al. Characterization of three new S-alleles and development of an S-allele-specific PCR system for rapidly identifying the S-genotype in apple cultivars. Tree Genetics & Genomes 6, 161–168 (2010). https://doi.org/10.1007/s11295-009-0237-6
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DOI: https://doi.org/10.1007/s11295-009-0237-6