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Expression profiling in HcrVf2-transformed apple plants in response to Venturia inaequalis

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Abstract

Apple scab resistance is one of the most well-characterized plant–pathogen interactions in a woody plant species. While the HcrVf2 gene from the wild apple Malus floribunda 821 has proved capable of conferring scab resistance to the susceptible cv. Gala after genetic transformation, its identification represents only the first step in understanding the molecular mechanisms and, hence, the network of genes underlying the defence response. We used a PCR-based suppression subtractive hybridization to identify apple genes that are differentially expressed after Venturia inaequalis inoculation. Subtractive hybridization was performed between cDNA from challenged leaves of HcrVf2-resistant transgenic Gala and susceptible cv. Gala plants. A library of 523 unigenes was constructed and characterized by assigning a putative function via comparison with public databases. This set of pathogen-modulated apple genes includes many defence-related genes and is therefore an important source of information for understanding the molecular basis of the Malus–V. inaequalis interaction.

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Acknowledgements

The authors thank Dott. Enrico Belfanti for the plant material, Dr. Gaetano Perrotta for his assistance during the sequencing of the SSH library and with sequence analysis and Dr. Vanina Ziosi for her help with the library screening.

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Authors and Affiliations

  1. Department of Fruit Tree and Woody Plant Sciences, University of Bologna, viale Fanin 46, 40127, Bologna, Italy

    Roberta Paris, Valentina Cova, Giulia Pagliarani, Stefano Tartarini & Silviero Sansavini

  2. Genetics and Molecular Biology Department, IASMA Research Centre, Via E. Mach 1-38010 S., Michele all’Adige (TN), Italy

    Valentina Cova & Matteo Komjanc

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  1. Roberta Paris
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  2. Valentina Cova
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  3. Giulia Pagliarani
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  4. Stefano Tartarini
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  6. Silviero Sansavini
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Correspondence to Roberta Paris.

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Communicated by J. Davis

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ESM 1

List of the 558 single-pass sequences of the apple SSH library (DOC 602 KB)

Table S1

List of the 334 unigenes identified by SSH and similar to known genes available in public databases. 264 clones were derived from the ‘forward’ subtraction, 59 from the ‘reverse’ and 11 from both the procedures (XLS 103 KB)

Table S2

List of ESTs that (1) did not match any sequence in the database (NO HIT), (2) match sequences of unknown function (UNCLASSIFIED); (3) match sequences of unclear function (UNCLEAR FUNCTION); (4) match transposon-related sequences (TRANSPOSONS) (XLS 58.0 KB)

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Paris, R., Cova, V., Pagliarani, G. et al. Expression profiling in HcrVf2-transformed apple plants in response to Venturia inaequalis . Tree Genetics & Genomes 5, 81–91 (2009). https://doi.org/10.1007/s11295-008-0177-6

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  • DOI: https://doi.org/10.1007/s11295-008-0177-6

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