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A framework physical map for peach, a model Rosaceae species

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Abstract

A genome-wide framework physical map of peach was constructed using high-information content fingerprinting (HICF) and FPC software. The resulting HICF assembly contained 2,138 contigs composed of 15,655 clones (4.3× peach genome equivalents) from two complementary bacterial artificial chromosome libraries. The total physical length of all contigs is estimated at 303 Mb or 104.5% of the peach genome. The framework physical map is anchored on the Prunus genetic reference map and integrated with the peach transcriptome map. The physical length of anchored contigs is estimated at 45.0 Mb or 15.5% of the genome. Altogether, 2,636 markers, i.e., genetic markers, peach unigene expressed sequence tags, and gene-specific and overgo probes, were incorporated into the physical framework and supported the accuracy of contig assembly.

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Acknowledgement

This project was supported by the United States Department of Agriculture NRI Award # 2005-35300-15452.

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Authors and Affiliations

  1. Department of Genetics and Biochemistry, Clemson University, Clemson, SC, 29634-0318, USA

    T. N. Zhebentyayeva, L. L. Georgi, L. Garay, S. Forrest, A. V. Blenda, J. Mook, R. Horn & A. G. Abbott

  2. Department of Horticulture, Clemson University, Clemson, SC, 29634-0319, USA

    G. Swire-Clark, W. V. Baird & G. L. Reighard

  3. Clemson University Genomics Institute, Clemson University, Clemson, SC, 29634, USA

    B. Blackmon & J. P. Tomkins

  4. Department of Horticulture and Landscape Architecture, Washington State University, Pullman, WA, 99164-6414, USA

    S. Jung & D. Main

  5. Centre de Recerca en Agrigenòmica CSIC–IRTA–UAB, Carretera de Cabrils Km 2, 08348, Cabrils, Barcelona, Spain

    W. Howad & P. Arús

  6. Department of Horticultural Sciences, North Carolina State University, Raleigh, NC, 2769-8619, USA

    B. Sosinski

  7. Agricultural Research Programs, Virginia State University, P.O. Box 9061, Petersburg, VA, 23806, USA

    J. Mook

  8. Abt Pflanzengenetik, Institut für Biowissenschaften, Universität Rostock, Albert-Einstein-Str 3, Rostock, 18051, Germany

    R. Horn

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  1. T. N. Zhebentyayeva
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  2. G. Swire-Clark
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  3. L. L. Georgi
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  4. L. Garay
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  5. S. Jung
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  6. S. Forrest
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  7. A. V. Blenda
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  8. B. Blackmon
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  9. J. Mook
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  10. R. Horn
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  11. W. Howad
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  12. P. Arús
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  13. D. Main
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  14. J. P. Tomkins
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  15. B. Sosinski
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  16. W. V. Baird
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  17. G. L. Reighard
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  18. A. G. Abbott
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Corresponding author

Correspondence to T. N. Zhebentyayeva.

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Communicated by R. Velasco

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Table 1

List of genetic markers integrated into peach HICF physical map (DOC 223 KB).

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Zhebentyayeva, T.N., Swire-Clark, G., Georgi, L.L. et al. A framework physical map for peach, a model Rosaceae species. Tree Genetics & Genomes 4, 745–756 (2008). https://doi.org/10.1007/s11295-008-0147-z

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  • DOI: https://doi.org/10.1007/s11295-008-0147-z

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