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Effect of photodynamic inactivation of Escherichia coli by hypericin

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World Journal of Microbiology and Biotechnology Aims and scope Submit manuscript

Abstract

The present study has focused on the effects of hypericin (Hyp) based photodynamic inactivation (PDI) of Escherichia coli (E. coli). To evaluate the efficiency of Hyp based PDI of E. coli, single factor experiments and response surface optimization experiment were conducted to obtain the optimum parameter values (36 µM Hyp, 5.9 J cm−2 light dose: 16.4 mW cm−2, 60 W, 260 s, 590 nm and 68 min incubation time) and finally achieved a 4.1 log CFU mL−1 decrease of E. coli. Cell-Hyp interaction and intracellular reactive oxygen species (ROS) level were detected by fluorescence spectrometric photometer. Data indicated that Hyp possessed a strong ability to bind with cells. In addition, a significant increase was observed in intracellular ROS level after Hyp-based photosensitization treatment. Therefore, Hyp-based photosensitization seems to be a promising method to efficiently inactivate E. coli. It is expected to be a safe, efficient, low cost and practical method which can be applied in the field of food safety.

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Abbreviations

Escherichia coli :

E. coli

PDT:

Photodynamic therapy

PDI:

Photodynamic inactivation

Hyp:

Hypericin

ROS:

Reactive oxygen species

LED:

Light-emitting diode

HPPL:

High power pulsed light

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Acknowledgements

This study was supported by the National Key R&D Project of China (2016YFD0400902).

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Author notes

  1. Jun-nan Zhang and Fang Zhang have contributed equally to this work.

Authors and Affiliations

  1. College of Food Science and Engineering, Ocean University of China, Qingdao, 266003, China

    Jun-nan Zhang, Fang Zhang, Qing-juan Tang & Xiang-hong Meng

  2. School of Chinese Medicine, The Chinese University of Hong Kong, Hong Kong, China

    Chuan-shan Xu

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  1. Jun-nan Zhang
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  2. Fang Zhang
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Correspondence to Xiang-hong Meng.

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Electronic supplementary material

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Supplementary Fig. S1. Schematic presentation of LED-based light source prototype (TIF 1079 KB)

11274_2018_2464_MOESM2_ESM.jpg

Supplementary Fig. S2. Curve fitting diagram of the relation between light density and light source power and light distance (JPG 599 KB)

11274_2018_2464_MOESM3_ESM.jpg

Supplementary Fig. S3. Inactivation of Escherichia coli by photosensitization with 5.9 J cm-2 as a function of anhydrous ethanol concentration (JPG 4407 KB)

Supplementary Fig. S4. The inhibition assessment of hypericin on RWPE-1 cells (JPG 69 KB)

11274_2018_2464_MOESM5_ESM.jpg

Fig. S5. Assessment of apoptosis in RWPE-1 Cells treated with 36 μM hypericin by using DAPI and PI staining through fluorescence microscope detection analysis (JPG 397 KB)

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Zhang, Jn., Zhang, F., Tang, Qj. et al. Effect of photodynamic inactivation of Escherichia coli by hypericin. World J Microbiol Biotechnol 34, 100 (2018). https://doi.org/10.1007/s11274-018-2464-1

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