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Characterization and evaluation of Bacillus amyloliquefaciens strain WF02 regarding its biocontrol activities and genetic responses against bacterial wilt in two different resistant tomato cultivars

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Abstract

Bacillus amyloliquefaciens strain WF02, isolated from soil collected at Wufeng Mountain, Taiwan, has siderophore-producing ability and in vitro antagonistic activity against bacterial wilt pathogen. To determine the impact of plant genotype on biocontrol effectiveness, we treated soil with this strain before infecting susceptible (L390) and moderately resistant (Micro-Tom) tomato cultivars with Ralstonia solanacearum strain Pss4. We also compared the efficacy of this strain with that of commercial Bacillus subtilis strain Y1336. Strain WF02 provided longer lasting protection against R. solanacearum than did strain Y1336 and controlled the development of wilt in both cultivars. To elucidate the genetic responses in these plants under WF02 treatment, we analyzed the temporal expression of defense-related genes in leaves. The salicylic acid pathway-related genes phenylalanine ammonia-lyase and pathogenesis-related protein 1a were up-regulated in both cultivars, whereas expression of the jasmonic acid pathway-related gene lipoxygenase was only elevated in the susceptible tomato cultivar (L390). These results suggest that WF02 can provide protection against bacterial wilt in tomato cultivars with different levels of disease resistance via direct and indirect modes of action.

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Acknowledgments

We appreciate Dr. Chiu-Ping Cheng (Graduate Institute of Plant Biology, National Taiwan University) and Dr. Jen-Chih Chen (Institute of Biotechnology, National Taiwan University) for sharing the bacterial and plant materials used in this study. We thank American Journal Experts for English language (Akila et al. 2011) editing. This study was supported by Grants from the Ministry of Science and Technology (101-2324-B-001-CC2 and 102-2313-B-002 -011 -MY3), National Taiwan University (102/103.SC 2B4 and 103R7602B429) and was also funded by Great Victory Chemical Industry Co. LTD.

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    Authors and Affiliations

    1. Master’s Program for Plant Medicine, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei, 106, Taiwan

      Chu-Ning Huang

    2. Department of Plant Pathology and Microbiology, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei, 106, Taiwan

      Chan-Pin Lin

    3. Institute of Biotechnology, National Taiwan University, R412., No. 81, Chang-Xing St., Taipei, 106, Taiwan

      Chan-Pin Lin, Sook-Kuan Lee, Kuan-Chen Cheng & Chi-Te Liu

    4. Biopesticides Division, Taiwan Agricultural Chemicals and Toxic Substances, Research Institute, Council of Agriculture, No. 11, Guangming Rd., Wufeng, Taichung, 413, Taiwan

      Feng-Chia Hsieh

    5. Graduate Institute of Food Science and Technology, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei, 106, Taiwan

      Kuan-Chen Cheng

    6. Agricultural Biotechnology Research Center, Academia Sinica, No. 128 Sec. 2, Academia Rd., Nankang, Taipei, 115, Taiwan

      Kuan-Chen Cheng & Chi-Te Liu

    7. Department of Medical Research, China Medical University Hospital, China Medical University, Taichung, 404, Taiwan

      Kuan-Chen Cheng

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    Correspondence to Chi-Te Liu.

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    Chu-Ning Huang and Chan-Pin Lin contributed equally to this work.

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    Huang, CN., Lin, CP., Hsieh, FC. et al. Characterization and evaluation of Bacillus amyloliquefaciens strain WF02 regarding its biocontrol activities and genetic responses against bacterial wilt in two different resistant tomato cultivars. World J Microbiol Biotechnol 32, 183 (2016). https://doi.org/10.1007/s11274-016-2143-z

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