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Evaluation of economically feasible, natural plant extract-based microbiological media for producing biomass of the dry rot biocontrol strain Pseudomonas fluorescens P22Y05 in liquid culture

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Abstract

The production of microbial biomass in liquid media often represents an indispensable step in the research and development of bacterial and fungal strains. Costs of commercially prepared nutrient media or purified media components, however, can represent a significant hurdle to conducting research in locations where obtaining these products is difficult. A less expensive option for providing components essential to microbial growth in liquid culture is the use of extracts of fresh or dried plant products obtained by using hot water extraction techniques. A total of 13 plant extract-based media were prepared from a variety of plant fruits, pods or seeds of plant species including Allium cepa (red onion bulb), Phaseolus vulgaris (green bean pods), and Lens culinaris (lentil seeds). In shake flask tests, cell production by potato dry rot antagonist Pseudomonas fluorescens P22Y05 in plant extract-based media was generally statistically indistinguishable from that in commercially produced tryptic soy broth and nutrient broth as measured by optical density and colony forming units/ml produced (P ≤ 0.05, Fisher’s protected LSD). The efficacy of biomass produced in the best plant extract-based media or commercial media was equivalent in reducing Fusarium dry rot by 50–96 % compared to controls. In studies using a high-throughput microbioreactor, logarithmic growth of P22Y05 in plant extract-based media initiated in 3–5 h in most cases but specific growth rate and the time of maximum OD varied as did the maximum pH obtained in media. Nutrient analysis of selected media before and after cell growth indicated that nitrogen in the form of NH4 accumulated in culture supernatants, possibly due to unbalanced growth conditions brought on by a scarcity of simple sugars in the media tested. The potential of plant extract-based media to economically produce biomass of microbes active in reducing plant disease is considerable and deserves further research.

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Acknowledgments

The assistance of Maggie Hammar and Kristina Glenzinski in conducting experiments is greatly appreciated. Studies were made possible, in part, by funding and travel support provided by the Higher Education Commission (HEC), Pakistan.

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Authors and Affiliations

  1. Department of Microbiology, University of Karachi, Karachi, 75270, Pakistan

    Sadia Khalil & Tasneem Adam Ali

  2. National Center for Agricultural Utilization Research (NCAUR), United States Department of Agriculture–Agricultural Research Service (USDA-ARS), 1815 N. University Street, Peoria, IL, 61604, USA

    Chris Skory, Patricia J. Slininger & David A. Schisler

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  1. Sadia Khalil
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  2. Tasneem Adam Ali
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  3. Chris Skory
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  4. Patricia J. Slininger
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  5. David A. Schisler
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Correspondence to David A. Schisler.

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Disclaimer: Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. USDA is an equal opportunity provider and employer.

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Khalil, S., Ali, T.A., Skory, C. et al. Evaluation of economically feasible, natural plant extract-based microbiological media for producing biomass of the dry rot biocontrol strain Pseudomonas fluorescens P22Y05 in liquid culture. World J Microbiol Biotechnol 32, 25 (2016). https://doi.org/10.1007/s11274-015-1984-1

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  • DOI: https://doi.org/10.1007/s11274-015-1984-1

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