Skip to main content
SpringerLink
Log in

Aerobic decolorization of Acid Brilliant Scarlet GR by microbial community and the community dynamics during sequencing batch processes

  • Original Paper
  • Published:
World Journal of Microbiology and Biotechnology Aims and scope Submit manuscript

Abstract

In this research, aerobic decolorization of Acid Brilliant Scarlet GR by microbial community was studied. Effects of conditions and dye concentraion on decolorization processes were investigated. Additionally, continuous decolorization was evaluated through sequencing batch tests and the microbial dynamics during this process was analyzed by polymerase chain reaction-denaturing gradient gel electrophoresis. The results showed that 100 mg l−1 of the dye was completely decolorized within 12 h, which was mainly caused by biodegradation. The optimal decolorization conditions were as follows: inoculation size 2.07 g l−1 (wet cell pellet), rotation speed 150 r min−1, pH 5.0–7.0 and 30 °C. The processes were well described by zero-order kinetics, and more than 700 mg l−1 of the dye would inhibit the activity of the consortium. Furthermore, the microbial community exhibited high efficiency in sequencing batch processes for continuous decolorization. Microbial community structure shifted obviously when exposed to higher concentration of the dye (500 mg l−1), and all the dominant microorganisms were affiliated with four different phyla of Actinobacteria, Bacteroidetes, Proteobacteria and Firmicutes.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

References

  • Benson DA, Karsch-Mizrachi I, Lipman DJ, Ostell J, Rapp BA, Wheeler DL (2002) GenBank. Nucleic Acids Res 30(1):17–20

    Article  CAS  Google Scholar 

  • Buitrón G, Quezada M, Moreno G (2004) Aerobic degradation of the azo dye acid red 151 in a sequencing batch biofilter. Bioresour Technol 92(2):143–149

    Article  Google Scholar 

  • Davies LC, Pedro IS, Novais JM, Martins-Dias S (2006) Aerobic degradation of Acid Orange 7 in a vertical-flow constructed wetland. Water Res 40(10):2055–2063

    Article  CAS  Google Scholar 

  • He Z, Lin L, Song S, Xia M, Xu L, Ying H, Chen J (2008) Mineralization of C. I. Reactive blue 19 by ozonation combined with sonolysis: performance optimization and degradation mechanism. Sep Purif Technol 62(2):376–381

    Article  CAS  Google Scholar 

  • Jabari L, Gannoun H, Cayol JL, Hedi A, Sakamoto M, Falsen E, Ohkuma M, Hamdi M, Fauque G, Ollivier B, Fardeau ML (2012) Macellibacteroides fermentans gen. nov., sp. nov., a member of the family Porphyromonadaceae isolated from an upflow anaerobic filter treating abattoir wastewaters. Int J Syst Evol Microbiol 62(Pt10):2522–2527

    Google Scholar 

  • Kalyani DC, Patil PS, Jadhav JP, Govindwar SP (2008) Biodegradation of reactive textile dye Red BLI by an isolated bacterium Pseudomonas sp. SUK1. Bioresour Technol 99(11):4635–4641

    Article  CAS  Google Scholar 

  • Kumar K, Dastidar MG, Sreekrishnan TR (2009) Effect of process parameters on aerobic decolourization of reactive azo dye using mixed culture. World Acad Sci Eng Tech 58:962–965

    Google Scholar 

  • Maier J, Kandelbauer A, Erlacher A, Cavaco-Paulo A, Gübitz GM (2004) A new alkali-thermostable azoreductase from Bacillus sp. strain SF. Appl Environ Microbiol 70(2):837–844

    Article  CAS  Google Scholar 

  • Mitter EK, Corso CR (2012) Acid Black 48 dye biosorption using Saccharomyces cerevisiae immobilized with treated sugarcane bagasse. Water Sci Technol 66(7):1431–1438

    Article  CAS  Google Scholar 

  • Modi HA, Rajput G, Ambasana C (2010) Decolorization of water soluble azo dyes by bacterial cultures, isolated from dye house effluent. Bioresour Technol 101(16):6580–6583

    Article  CAS  Google Scholar 

  • Morais PV, Francisco R, Branco R, Chung AP, da Costa MS (2004) Leucobacter chromiireducens sp. nov, and Leucobacter aridicollis sp. nov., two new species isolated from a chromium contaminated environment. Syst Appl Microbiol 27(6):646–652

    Article  CAS  Google Scholar 

  • Nachiyar CV, Rajakumar GS (2005) Purification and characterization of an oxygen insensitive azoreductase from Pseudomonas aeruginosa. Enzyme Microb Technol 36(4):503–509

    Article  CAS  Google Scholar 

  • Ooi T, Shibata T, Sato R, Ohno H, Kinoshita S, Thuoc TL, Taguchi S (2007) An azoreductase, aerobic NADH-dependent flavoprotein discovered from Bacillus sp.: functional expression and enzymatic characterization. Appl Microbiol Biotechnol 75(2):377–386

    Article  CAS  Google Scholar 

  • Ozturk A, Abdullah MI (2006) Toxicological effect of indole and its azo dye derivatives on some microorganisms under aerobic conditions. Sci Total Environ 358(1–3):137–142

    Article  Google Scholar 

  • Pagga U, Taeger K (1994) Development of a method for adsorption of dyestuffs on activated sludge. Water Res 28(5):1051–1057

    Article  CAS  Google Scholar 

  • Pandey A, Singh P, Iyengar L (2007) Bacterial decolorization and degradation of azo dyes. Int Biodeterior Biodegrad 59(2):73–84

    Article  CAS  Google Scholar 

  • Pant D, Adholeya A (2009) Concentration of fungal ligninolytic enzymes produced during solid-state fermentation by ultrafiltration. World J Microb Biot 25(10):1793–1800

    Article  CAS  Google Scholar 

  • Pant D, Adholeya A (2010) Development of a novel fungal consortium for the treatment of molasses distillery wastewater. Environmentalist 30(2):178–182

    Article  Google Scholar 

  • Pant D, Singh A, Satyawali Y, Gupta RK (2008) Effect of carbon and nitrogen source amendment on synthetic dyes decolourizing efficiency of white-rot fungus Phanerochaete chrysosporium. J Environ Biol 29(1):79–84

    CAS  Google Scholar 

  • Pearce CI, Lloyd JR, Guthrie JT (2003) The removal of colour from textile wastewater using whole bacterial cells: a review. Dyes Pigm 58(3):179–196

    Article  CAS  Google Scholar 

  • Pourbabaee AA, Malekzadeh F, Sarbolouki MN, Najafi F (2006) Aerobic decolorization and detoxification of a disperse dye in textile effluent by a new isolate of Bacillus sp. Biotechnol Bioeng 93(4):631–635

    Article  CAS  Google Scholar 

  • Qu Y, Zhou J, Wang J, Song Z, Xing L, Fu X (2006) Bioaugmentation of bromoamine acid degradation with Sphingomonas xenophaga QYY and DNA fingerprint analysis of augmented systems. Biodegradation 17(1):83–91

    Article  CAS  Google Scholar 

  • Qu Y, Zhang Q, Wei L, Ma F, Zhou J, Pi W, Gou M (2009) Optimization of metagenomic DNA extraction from activated sludge samples. Asia-Pac J Chem Eng 4(5):780–786

    Article  CAS  Google Scholar 

  • Rajkumar D, Song BJ, Kim JG (2007) Electrochemical degradation of Reactive blue 19 in chloride medium for the treatment of textile dyeing wastewater with identification of intermediate compounds. Dyes Pigm 72(1):1–7

    Article  Google Scholar 

  • Ruiz-Arias A, Juárez-Ramírez C, de los Cobos-Vasconcelos D, Ruiz-Ordaz N, Salmerón-Alcocer A, Ahuatzi-Chacón D, Galíndez-Mayer J (2010) Aerobic biodegradation of a sulfonated phenylazonaphthol dye by a bacterial community immobilized in a multistage packed-bed BAC reactor. Appl Biochem Biotechnol 162(2):1689–1707

    Google Scholar 

  • Sandhya S, Padmavathy S, Swaminathan K, Subrahmanyam YV, Kaul SN (2005) Microaerophilic-aerobic sequential batch reactor for treatment of azo dyes containing simulated wastewater. Process Biochem 40(2):885–890

    Article  CAS  Google Scholar 

  • Stams AJ, Huisman J, Garcia Encina PA, Muyzer G (2009) Citric acid wastewater as electron donor for biological sulfate reduction. Appl Microbiol Biotechnol 83(5):957–963

    Article  CAS  Google Scholar 

  • Tan L, Qu Y, Zhou J, Ma F, Li A (2009) Dynamics of microbial community for X-3B wastewater decolorization coping with high-salt and metal ions conditions. Bioresour Technol 100(12):3003–3009

    Article  CAS  Google Scholar 

  • Tan HQ, Li TT, Zhu C, Zhang XQ, Wu M, Zhu XF (2012) Parabacteroides chartae sp. nov., an obligately anaerobic species from wastewater of a paper mill. Int J Syst Evol Microbiol 62(Pt11):2613–2617

    Google Scholar 

  • Tan L, Ning S, Wang Y, Cao X (2012b) Influence of dye type and salinity on aerobic decolorization of azo dyes by microbial consortium and the community dynamics. Water Sci Technol 65(8):1375–1382

    Article  Google Scholar 

  • Teske A, Wawer C, Muyzer G, Ramsing NB (1996) Distribution of sulfate-reducing bacteria in a stratified fjord (Mariager Fjord, Denmark) as evaluated by most-probable-number counts and denaturing gradient gel electrophoresis of PCR-amplified ribosomal DNA fragments. Appl Environ Microbiol 62(4):1405–1415

    CAS  Google Scholar 

  • van der Zee FP, Villaverde S (2005) Combined anaerobic–aerobic treatment of azo dyes—a short review of bioreactor studies. Water Res 39(8):1425–1440

    Article  Google Scholar 

  • Zhou J, Xu Y, Qu Y, Tan L (2010) Decolorization of Brilliant Scarlet GR enhanced by bioaugmentation and redox mediators under high-salt conditions. Bioresour Technol 101(2):586–591

    Article  CAS  Google Scholar 

Download references

Acknowledgments

The work was financially supported by the National Natural Science Foundation of China (No. 51108223). The authors also gratefully acknowledge the support of Chinese Academy of Sciences, Shenyang Institute of Applied Ecology (CAS SIAE).

Author information

Authors and Affiliations

  1. School of Life Science, Liaoning Normal University, Dalian, 116081, China

    Liang Tan, Shuxiang Ning & Ying Wang

  2. Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education), School of Environmental Science and Technology, Dalian University of Technology, Dalian, 116024, China

    Xiangyu Cao

Authors
  1. Liang Tan
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Shuxiang Ning
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Ying Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Xiangyu Cao
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Liang Tan.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Tan, L., Ning, S., Wang, Y. et al. Aerobic decolorization of Acid Brilliant Scarlet GR by microbial community and the community dynamics during sequencing batch processes. World J Microbiol Biotechnol 29, 1763–1771 (2013). https://doi.org/10.1007/s11274-012-1249-1

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11274-012-1249-1

Keywords

Search

Navigation