Abstract
Biosurfactant-producing bacteria were isolated from mangrove sediment in southern Thailand. Isolates were screened for biosurfactant production by using the surface tension test. The highest reduction of surface tension was achieved with a bacterial strain which was identified by 16S rRNA gene sequencing as Oleomonas sagaranensis AT18. It has also been investigated using different carbon and nitrogen sources. It showed that the strain was able to grow and reduce the surface tension of the culture supernatant to 25 mN/m. In all 5.30 g of biosurfactant yield was obtained after 54 h of cultivation by using molasses and NaNO3 as carbon and nitrogen sources, respectively. The biosurfactant recovery by chloroform:methanol extraction showed a small critical micelle concentration value (8 mg/l), thermal and pH stability with respect to surface tension reduction. It also showed emulsification activity and a high level of salt concentration. The biosurfactant obtained was confirmed as a glycolipid by using a biochemical test, FT-IR and mass spectra. The crude biosurfactant showed a broad spectrum of antimicrobial activity and also had the ability to emulsify oil and enhance PAHs solubility.
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Abdel-Mawgoud AM, Lepine F, Deziel E (2010) Rhamnolipids: diversity of structures, microbial origins and roles. Appl Microbiol Biotechnol 86:1323–1336
Anandaraj B, Thivakaran P (2010) Isolation and production of biosurfactant producing organism from oil spilled soil. Biosci Tech 1:120–126
Anderson RC, Yu PK (2005) Factors affecting the antimicrobial activity of ovine-dervied cathelicidins aginst Escherichia coli O157:H7. Int J Antimicrob Ag 25:205–210
Banat IM, Makkar RS, Cameotra SS (2000) Potential commercial applications of microbial surfactants. Appl Microbiol Biotechnol 53:495–508
Barber WP, Stuckey DC (2000) Nitrogen removal in a modified anaerobic baffled reactor (ABR): 1, denitrification. Water Res 10:2413–2422
Barkay T, Navon-Venezia S, Ron EZ, Rosenberg E (1999) Enhancement of solubilization and biodegradation of polyaromatic hydrocarbons by the bioemulsifier alasan. Appl Environ Microbiol 65:2697–2702
Bodour AA, Drees KP, Raina MM (2003) Distribution of biosurfactant-producing bacteria in undisturbed and contaminated arid Southwestern soils. Appl Environ Microbiol 69:3280–3287
Brown LR (2010) Microbial enhanced oil recovery (MEOR). Curr Opin Microbiol 13:316–320
Burgos-Diaz C, Pons R, Espuny MJ, Aranda FJ, Teruel JA, Manresa A, Ortiz A, Marques AM (2011) Isolation and partial characterization of a biosurfactant mixture produced by Sphingobacterium sp. isolated from soil. J Colloid Interface Sci 361:195–204
Candan F, Unlu M, Tepe B, Daferera D, Polissiou M, Sokmen A, Akpulat HA (2003) Antioxidant and antimicrobial activity of the essential oil and methanol extracts of Achillea millefolium subsp. millefolium Afan. (Asteraceae). J Ethnopharmacol 87:215–220
Chayabutra C, Wu J, Ju LK (2001) Rhamnolipid production by Pseudomonas aeruginosa under denitrification: effects of limiting nutrients and carbon substrates. Biotechnol Bioeng 72:25–33
Cooper DG, Goldenberg BG (1987) Surface-active agents from two Bacillus species. Appl Environ Microbiol 53:224–229
Das P, Mukherjee S, Sen R (2008) Antimicrobial potential of a lipopeptide biosurfactant derived from a marine Bacillus circulans. J Appl Microbiol 104:1675–1684
Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F (1956) Colorimetric method for determination of sugars and related substances. Anal Chem 28:350–356
Fathabad EG (2011) Biosurfactants in pharmaceutical industry. Am J Drug Discov Dev 1:58–69
Folch JM, Lees M, Stanly HS (1956) A simple method for the isolation and quantification of total lipids from animal tissues. J Biol Chem 226:497–509
Franzetti A, Caredda P, Colla PL, Pintus M, Tamburini E, Papacchini M, Bestetti G (2009) Cultural factors affecting biosurfactant production by Gordonia sp. BS29. Int Biodeterior Biodegrad 63:943–947
Gudina EJ, Teixeira JA, Rodrigues LR (2010) Isolation and functional characterization of a biosurfactant produced by Lactobacillus paracasei. Colloid Surf B 76:298–304
Haddad NI, Wang J, Mu B (2008) Isolation and characterization of a biosurfactant producing strain, Brevibacilis brevis HOB1. J Ind Microbiol Biotechnol 35:1597–1604
Ilori MO, Amobi CJ, Odocha AC (2005) Factors affecting biosurfactant production by oil degrading Aeromonas sp., isolated from a tropical environment. Chemosphere 61:985–992
Intergovernmental oceanographic commission manuals and guide No.13 (1982) Manual for monitoring oil and dissolved/dispersed petroleum hydrocarbons in marine waters and beaches. UNESCO
Janek T, Lukaszewicz M, Rezanka T, Krasowska A (2010) Isolation and characterization of two new lipopeptide biosurfactants produced by Pseudomonas fluorescens BD5 isolated from water from the arctic archipelago of Svalbard. Bioresour Technol 101:6118–6123
Ke L, Wang WQ, Wong TW, Wong YS, Tam NF (2003) Removal of pyrene from contaminated sediments by mangrove microcosms. Chemosphere 52:1581–1591
Kim SY, Kim JY, Kim SH, Bae HJ, Yi H, Yoon SH, Koo BS, Kwon M, Cho JY, Lee CE, Hong S (2007) Surfactin from Bacillus subtilis displays anti-proliferative effect via apoptosis induction, cell cycle arrest and survival signaling suppression. FEBS Lett 581:865–871
Kuyukina MS, Ivshina IB, Makarov SO, Litvinenko LV, Cunningham CJ, Philip JC (2005) Effect of biosurfactants on crude oil desorption and mobilization in a soil system. Environ Int 31:155–161
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275
Maneerat S (2009) Biosurfactants from marine microorganisms. Songklanakarin J Sci Technol 27:1263–1272
Mulligan CN (2009) Recent advances in the environmental applications of biosurfactants. Curr Opin Colloid Interface Sci 14:372–378
Negi PS, Chauhan AS, Sadia GA, Rohinishree YS, Ramteke RS (2005) Antioxidant and antimicrobial activities of various seabuckthorn (Hippophae rhamnoides L.) seed extracts. Food Chem 92:119–124
Nilsson WB, Strom MS (2002) Detection and identification of bacterial pathogens of fish in kidney tissue using terminal restriction length polymorphism (T-RFLP) analysis of 16S rRNA genes. Dis Aquat Org 48:175–185
Nitschke M, Coast SG (2007) Biosurfactants in food industry. Trends Food Sci Technol 18:252–259
Nitschke M, Pastore G (2006) Production and properties of a surfactant obtained from Bacillus subtilis grown on cassava wastewater. Bioresour Technol 97:336–341
Obayori O, Ilori M, Adebusoye S, Oyetibo G, Omotayo A, Amund O (2009) Degradation of hydrocarbons and biosurfactant production by Pseudomonas sp. strain LP1. World J Microbiol Biotechnol 25:1615–1623
Phalakornkule C, Tanasupawat S (2006) Characterization of lactic acid bacteria from traditional Thai sausages. J Cult Collect 5:46–57
Prieto LM, Michelon M, Burkert JFM, Kalil SJ, Burkert CAV (2008) The production of rhamnolipid by a Pseudomonas aeruginosa strain isolated from a southern coastal zone in Brazil. Chemosphere 71:1781–1785
Qiao N, Shao Z (2010) Isolation and characterization of a novel biosurfactant produced by hydrocarbon-degrading bacterium Alcanivorax dieselolei B-5. J Appl Microbiol 108:1207–1216
Saimmai A, Sobhon V, Maneerat S (2011) Molasses a whole medium for bosurfactants production by Bacillus strains and their application. Appl Biochem Biotechnol 165:315–335
Saimmai A, Sobhon V, Maneerat S (2012) Production of biosurfactant from a new and promising strain of Leucobacter komagatae 183. Ann Microbiol 62:391–402
Sangster J (1989) Octanol-water partition coefficients of simple organic compounds. J Phys Chem Ref Data 18:3
Silva SNRL, Farias CBB, Rufino RD, Luna JM, Sarubbo LA (2010) Glycerol as substrate for the production of biosurfactant by Pseudomonas aeruginosa UCP0992. Colloid Surf B 79:174–183
Snape I, Ferguson SH, Harvey PM, Riddle MJ (2006) Investigation of evaporation and biodegradation of fuel spills in Antarctica: II extent of natural attenuation at Casey station. Chemosphere 63:89–98
Sobrinho HBS, Rufino RD, Luna JM, Salgueiro AA, Campos-Takaki GM, Leite LFC, Sarubbo LA (2008) Utilization of two agroindustrial by-products for the production of a surfactant by Candida sphaerica UCP0995. Process Biochem 43:912–917
Tamura K, Dudley J, Nei M, Kumar S (2007) MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol Biol Evol 24:1596–1599
Thanomsub B, Pumeechockchai W, Limtrakul A, Arunrattiyakorn P, Petchleelaha W, Nitoda T, Kanzaki H (2007) Chemical structure and biological activities of rhamnolipids produced by Pseudomonas aeruginosa B189 isolated from milk factory waste. Bioresour Technol 98:1149–1153
Thavasi R, Jayalakshmi S, Balasubramanian T, Banat IM (2007) Biosurfactant production by Corynebacterium kutscheri from waste motor lubricant oil and peanut oil cake. Lett Appl Microbiol 45:686–691
Thavasi R, Subramanyam Nambaru VRM, Jayalakshmi S, Balasubramanian T, Banat IM (2011) Biosurfactant production by Pseudomonas aeruginosa from renewable resources. Indian J Microbiol 51:30–36
Thompson JD, Gibbons TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) The CLUSTAL X windows interfac: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882
Van Bogaert INA, Zhang J, Soetaert W (2011) Microbial synthesis of sophorolipids. Process Biochem 46:821–833
Wei YH, Chou CL, Chang JS (2005) Rhamnolipid production by indigenous Pseudomonas aeruginosa J4 originating from petrochemical wastewater. J Biochem Eng 27:146–154
Wu JY, Yeh KL, Lu WB, Lin CL, Chang JS (2008) Rhamnolipid production with indigenous Pseudomonas aeruginosa EM1 isolated from oil-contaminated site. Bioresour Technol 99:1157–1164
Acknowledgments
We are grateful to Phuket Rajabhat University for providing a scholarship to A. S. This work was supported by the Higher Education Research Promotion and National Research University Project of Thailand, Office of the Higher Education Commission. This work was also funded by the Graduate School, Prince of Songkla University.
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Saimmai, A., Rukadee, O., Onlamool, T. et al. Isolation and functional characterization of a biosurfactant produced by a new and promising strain of Oleomonas sagaranensis AT18. World J Microbiol Biotechnol 28, 2973–2986 (2012). https://doi.org/10.1007/s11274-012-1108-0
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DOI: https://doi.org/10.1007/s11274-012-1108-0