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Analysis and comparison of the microflora isolated from fresco surface and from surrounding air environment through molecular and biodegradative assays

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Abstract

The aim of this study was to find a correlation among the environmental isolated microflora and the fresco colonizators through the investigation of their biodegradative abilities and DNA characteristics. A molecular technique named RAMP (Random Amplified Microsatellite Polymorphisms) was utilized in order to analyze the DNA diversity of bacterial and fungal species isolated from fresco as well as from air samples. The RAMP-PCR results were combined with the screening of some biodegradative properties obtained through the use of specific agar plate assays detecting the proteolytic, solubilization and biomineralization abilities of the isolated microflora. This comparative analysis showed that only in few cases a direct link among the fresco and airborne isolates of specific microbial group existed. The investigation clearly evidenced that colonization of surface of Ladislav’s fresco occurred in different time and by different strains than those observed at the moment of sampling campaign. Furthermore, the microflora investigation permitted the identification of taxonomically interesting bacteria with particular biodegradative properties, which had been less studied until now.

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Acknowledgments

The research was carried out in the frame of the Bilateral Project CNR-SAV project nr. 2/UNI11 entitled “Molecular Characterization of Microbial Communities involved in the Biodegradation of Cultural Heritage”. The work was also partially supported by the VEGA project nr. 2/0117/08: “Biorestoration: complex analysis of environmental microflora applied to innovative stone artwork restoration techniques” and by the University of Messina (P.R.A. 2008–2010).

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Authors and Affiliations

  1. Institute of Molecular Biology, Slovak Academy of Sciences, Dúbravská cesta 21, 84551, Bratislava, Slovak Republic

    Domenico Pangallo, Lucia Kraková & Katarína Chovanová

  2. Caravella s.r.o., Tupolevova 2, 851 01, Bratislava, Slovak Republic

    Domenico Pangallo

  3. Department of Soil Science, Faculty of Natural Sciences, Comenius University, Mlynska dolina B-2, 84215, Bratislava 4, Slovak Republic

    Alexandra Šimonovičová

  4. Department of Life Sciences, University of Messina, Viale F. Stagno d’Alcontres, 31, 98166, Messina, Italy

    Filomena De Leo & Clara Urzì

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  1. Domenico Pangallo
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  2. Lucia Kraková
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  3. Katarína Chovanová
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  4. Alexandra Šimonovičová
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  5. Filomena De Leo
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  6. Clara Urzì
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Correspondence to Domenico Pangallo.

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Pangallo, D., Kraková, L., Chovanová, K. et al. Analysis and comparison of the microflora isolated from fresco surface and from surrounding air environment through molecular and biodegradative assays. World J Microbiol Biotechnol 28, 2015–2027 (2012). https://doi.org/10.1007/s11274-012-1004-7

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  • DOI: https://doi.org/10.1007/s11274-012-1004-7

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