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In vitro evaluation of the effect of the nematophagous fungi Duddingtonia flagrans, Monacrosporium sinense and Pochonia chlamydosporia on Schistosoma mansoni eggs

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Abstract

The in vitro effect of four isolates of the nematophagous fungi Duddingtonia flagrans (AC 001), Monacrosporium sinense (SF 53) and Pochonia chlamydosporia (VC 1 and VC 4) on eggs of Schistosoma mansoni was examined. One thousand S. mansoni eggs were plated on 2% water–agar with the grown isolates and control without fungus. After 7, 14 and 21 days, the eggs were removed and classified according to the following parameters: type 1, lytic effect without morphological damage to eggshell; type 2, lytic effect with morphological alteration of embryo and eggshell; and type 3, lytic effect with morphological alteration of embryo and eggshell, besides hyphal penetration and internal egg colonization. Significant differences (P < 0.01) were found among the studied fungal isolates for ovicidal activity, confirming type 3 effect for the isolates VC 1 and VC 4, which characterizes the ovicidal activity of a fungus. Type 3 effect was only found for P. chlamydosporia (VC 1 and VC 4) with 26.6 and 17.2%, 25.6 and 22.6%, 27.4 and 23.9% in the 7, 14 and 21 days respectively (P < 0.01). P. chlamydosporia can thus be a potential biological control agent for S. mansoni eggs.

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Acknowledgements

The work has a financial support of CNPq.

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Authors and Affiliations

  1. Departamento de Veterinária, Universidade Federal de Viçosa, Vicosa, MG, 36570-000, Brazil

    F. R. Braga, J. V. Araújo, A. R. Silva, J. M. Araujo & R. O. Carvalho

  2. CNPq Scholarship, Sao Paulo, Brazil

    J. V. Araújo

  3. UNIVIÇOSA, Vicosa, MG, Brazil

    A. K. Campos

  4. Departamento de Parasitologia, Instituto de Ciências Biológicas (ICB), Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, Brazil

    D. N. Corrêa & C. A. J. Pereira

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  1. F. R. Braga
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  2. J. V. Araújo
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  3. A. K. Campos
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  4. A. R. Silva
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  5. J. M. Araujo
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  6. R. O. Carvalho
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  7. D. N. Corrêa
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  8. C. A. J. Pereira
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Correspondence to J. V. Araújo.

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Braga, F.R., Araújo, J.V., Campos, A.K. et al. In vitro evaluation of the effect of the nematophagous fungi Duddingtonia flagrans, Monacrosporium sinense and Pochonia chlamydosporia on Schistosoma mansoni eggs. World J Microbiol Biotechnol 24, 2713–2716 (2008). https://doi.org/10.1007/s11274-008-9843-y

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  • DOI: https://doi.org/10.1007/s11274-008-9843-y

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