Summary
Process conditions for cell cultures derived from conchocelis of female red macroalga Porphyra haitanensis were optimized in an illuminated 0.3-l bubble-column photobioreactor, using CO2 in air as the sole carbon source during a 20-day cultivation period. It reached the highest growth rate when the initial cell density was 700 mg l−1 (dry weight), the optional aeration rate was 1.2 v/v/min, inorganic nitrate concentration was 15 mM and inorganic phosphate concentration was 0.6 mM. This is the first reported bioreactor cultivation study of cell cultures derived from conchocelis of Porphyra haitanensis.
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References
Chalmers J.J., 1994 Cells and bubbles in sparged bioreactors Cytotechnology 15: 311–320
Cheney D., Metz B., Stiller J., 2001 Agrobacterium-mediated genetic transformation in the macroscopic marine red alga Porphyra yezoensis Journal of Phycology 37 Suppl: S11
Conitz J.M., Fagen R., Lindstrom S.C., Plumley F.G., Stekoll M.S., 2001 Growth and pigmentation of juvenile Porphyra torta (Rhodophyta) gametophytes in response to nitrate, salinity and inorganic carton Journal of Applied Phycology 13: 423–431
Dinabandhu S., Tang X.-R., Charles Y., 2002 Porphyra – the economic seaweed as a new experimental system Current Science 83: 1313–1316
He P., Yao Q., Chen Q., Guo M., Xiong A., Wu W., Ma J., 2001 Transferring and expression of glucose oxidase gene – gluc in Porphyra yezoensis Journal of Phycology 37 Suppl S22
Hiroyuki N., 1993 Health benefits and nutritional properties of Nori Journal of Applied Phycology 5: 255–258
Javanmardian M., Palsson B.O., 1991 High-density photoautotrophic algal cultures: design, construction, and operation of a novel photobioreactor system Biotechnology and Bioengineering 38: 1182–1189
Kuang M., Wang S.-J., Li Y., Shen D.-L., Tseng C.-K., 1998 Transient expression of exogenous GUS gene in Porphyra yezoensis (Rhodophyta) Chinese Journal of Oceanology and Limnology 16: 56–61
Lin C.M., Yarish C., Chen T., 2004 Development of a novel gene transfer method in Porphyra Bulletin of Fish Research Agency (Suppl. 1): S155
Masanobu S., Masanobu K., Hiroshi H., Jun O., Kunio Y., Manabu H., Toshio I., 2002 Antihypertensive effect of Nori-peptides derived from red alga Porphyra yezoensis in hypertensive patients American Journal of Hypertension Supplement 1(15): 210
Merchuk J.C., 1991 Shear effects on suspended cells Advances in Biochemical Engineering 44: 65
Nelson W.A., Brodie J., Guiry M.D., 1999 Terminology used to describe reproduction and life history stages in the genus Porphyra (Bangiales, Rhodophyta) Journal of Applied Phycology 11: 407–410
Qi H., Rorrer G.L., 1995 Photolithotrophic cultivation of Laminaria saccharina gametophyte cells in a stirred-tank bioreactor Biotechnology and Bioengineering 45: 251–260
Rupérez P., 2002 Mineral content of edible marine seaweeds Food Chemistry 79: 23–26
Takahiro E., Kiyotaka N., Miki I., Teruo M., 2004 Telomerase inhibition by sulfoquinovosyldiacylglycerol from edible purple laver (Porphyra yezoensis) Cancer Letters 212: 15–20
Waaland J.R., Stiller J.W., Cheney D.P., 2004 Minireview – Macroalgal candidates for genomics Journal of Phycology 40: 26–33
Walther I., Bechler B., Müller O., Hunzinger E., Cogoli A., 1996 Cultivation of Saccharomyces cerevisiae in a bioreactor in microgravity Journal of Biotechnology 47: 113–127
Wang S., Wang G., Sun Y., Lu A., 1987 Isolation and cultivation of the vegetative cells of Porphyra haitanesis (Rhodophyta) Chinese Journal of Oceanology and Limnology 5: 333–339
Yashizawa Y., Enomoto A., Todoh H., Ametani A., Kaminogawa S., 1993 Activation of murine macrophages by polysaccharide fractions from marine alga (Porphyra yezoensis) Bioscience, Biotechnology, and Biochemistry 57: 1862–1866
Yashizawa Y., Ametani A., Tsunehiro J., Numura K., Itoh M., Fukui F., Kaminogawa S., 1995 Macrophage stimulation activity of the polysaccharide fraction from a marine alga (Porphyra yezoensis): structure–function relationships and improved solubility Bioscience, Biotechnology, and Biochemistry 59: 1933–1937
Zhang Q.-B., Li N., Zhou G.-F., Lu X.-L., Xu Z.-H., Li Z.-E., 2003a In vivo antioxidant activity of polysaccharide fraction from Porphyra haitanesis (Rhodephyta) in aging mice Pharmacological Research 48: 151–155
Zhang Q.-B., Li D.-X., Yu P.-Z., Liu X.-G., Li Z.-E., Xu Z.-H., 2003b Effect of polysaccharides from Porphyra haitanesis on the activity of spleen cells Chinese Journal of Marine Drugs 96: 14–18
Zhou H.-P., Chen Q.-H., 1990 Anticoagulant and antihyperlipedemic effects of polysaccharide from Porphyra yezoensis Journal of China Pharmaceutical University 21: 358–360
Acknowledgments
This work was supported by the grant from the Key Innovative Project of the Chinese Academy of Sciences (No. KZCX3-SW-215). The authors also thank Dr. JiangPeng for helpful discussions and Dr. YangRui for providing Porphyra haitanensis female conchocelis cells in this research.
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Zhang, W., Gao, JT., Zhang, YC. et al. Optimization of conditions for cell cultivation of Porphyra haitanensis conchocelis in a bubble-column bioreactor. World J Microbiol Biotechnol 22, 655–660 (2006). https://doi.org/10.1007/s11274-005-9050-z
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DOI: https://doi.org/10.1007/s11274-005-9050-z