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Ovine adenoviruses infecting sheep and goats in Türkiye: detection and molecular characterization of three different types

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Abstract

Adenoviruses (AdVs) have been detected in a wide variety of animals. To date, eight types of AdVs in sheep and two types in goats have been identified, which belong to two distinct genera, Mastadenovirus and Atadenovirus. Typically, the term pneumo-enteritis is used to describe adenovirus-induced disease in small ruminants, which has been associated with both enteric and respiratory symptoms of varying severity. The aim of this study was to detect and identify AdVs of small ruminants belonging to the genera Mastadenovirus and Atadenovirus. For this purpose, diagnostic samples (47 lung, 27 intestine, and two pooled tissue samples including intestine and lung) from 49 small ruminants (39 sheep and 10 goats) were used. Following the viral DNA extraction, PCR was carried out by using the primers targeting the hexon gene in order to detect both mast- and atadenoviruses. Sequencing the amplified fragments revealed the presence of three types of ovine adenovirus (OAdV): OAdV-3, OAdV-4, and OAdV-8. Specifically, OAdV-3 was detected in two sheep and a goat while OAdV-4 and OAdV-8 were found in only one sheep each. There is still limited data on the interaction between the viruses in different adenovirus genera and the detected disease, as well as the genetic diversity of adenoviruses, especially in small ruminants. In conclusion, the detection of AdVs in lung and intestinal tissues of small ruminants in this study suggests that these viruses may have contributed to the disease and/or predisposed to other agents.

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Fig. 1

Data availability

The data that support the findings of this study are available from the corresponding author upon reasonable request.

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Acknowledgements

Preliminary findings of this study were presented at the 7th International Congress on Advances in Veterinary Sciences & Technics, on July 26, 2022, in Aksaray, Türkiye. The authors thank Prof. Dr. M. Ozkan Timurkan for kindly providing the positive control.

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The authors received no specific funding for this work.

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Authors and Affiliations

  1. Department of Virology, Faculty of Veterinary Medicine, Ankara University, Ankara, Türkiye

    Ilke Karayel-Hacioglu & Feray Alkan

  2. Department of Virology, Graduate School of Health Sciences, Ankara University, Ankara, Türkiye

    Buket Gul & Deniz Acun Yildiz

  3. Veterinary Control Central Research Institute, Ankara, Türkiye

    Deniz Acun Yildiz

Authors
  1. Ilke Karayel-Hacioglu
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  2. Buket Gul
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  3. Deniz Acun Yildiz
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  4. Feray Alkan
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Contributions

IKH: Conceptualization, Methodology, Investigation, Formal analysis, Visualization, Writing – original draft, Writing – review & editing. BG: Investigation, Visualization, Writing – original draft. DAY: Investigation, Writing – original draft. FA: Conceptualization, Methodology, Supervision, Writing – original draft, Writing – review & editing. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Feray Alkan.

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Ethical approval

No ethical approval was required for this study. In this study, retrospective samples sent to our laboratory by field veterinarians for diagnostic purposes were used.

Competing interests

The authors declare no competing interests.

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Edited by Juergen Richt.

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Karayel-Hacioglu, I., Gul, B., Acun Yildiz, D. et al. Ovine adenoviruses infecting sheep and goats in Türkiye: detection and molecular characterization of three different types. Virus Genes 60, 309–313 (2024). https://doi.org/10.1007/s11262-024-02063-w

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