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Development of a reverse genetics system for a feline panleukopenia virus

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Abstract

Feline panleukopenia virus (FPV) infects cats and can be fatal to kittens. There is evidence that canine parvovirus originated from FPV, which makes FPV important in studies of the family Parvoviridae. In the present study, the entire genome of FPV strain HH-1/86 was converted into a full-length infectious clone (pFPV). The FPV strain HH-1/86 has a 5123-nt single stranded DNA genome with a Y-shaped inverted 3′ terminal repeat (ITR) and a U-shaped inverted 5′ ITR. Feline kidney cells (F81) were transfected with the pFPV clone which contained a genetic marker, and a rescued virus was obtained (rFPV). The rFPV was identified by its cytopathic effects, indirect immunofluorescence, growth curve analysis, western blot assay and hemagglutination, and was indistinguishable from the parent virus. The FPV infectious clone will facilitate the study of pathogenicity and viral replication of FPV and the inter-species transmission of parvoviruses.

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Acknowledgements

This work was supported in part by the National Key Research and Development Program of China (No. 2016YFD0501004).

Funding

Funding was provided by National Natural Science Foundation of Jilin Province and Public Welfare (Agricultural) Industry Research Special Program.

Author information

Author notes

  1. Nan Cheng and Yongkun Zhao contributed equally to the results of this study.

Authors and Affiliations

  1. Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Science, 666 Liuying Xi Road, Changchun, 130122, China

    Nan Cheng, Yongkun Zhao, Qiuxue Han, Weijiao Zhang, Hualei Wang, Guohua Li, Yuwei Gao, Songtao Yang & Xianzhu Xia

  2. College of Veterinary Medicine, China Agricultural University, Beijing, China

    Nan Cheng

  3. College of Biological Sciences, China Agricultural University, Beijing, China

    Ji Xi, Yongle Yu & Weiquan Liu

  4. Jiangsu Co-innovation Centre for Prevention and Control of Important Animal Infectious Disease and Zoonosis, Yangzhou, China

    Yongkun Zhao, Hualei Wang, Yuwei Gao, Songtao Yang & Xianzhu Xia

  5. College of Animal Science and Technology, Shihezi University, Shihezi, China

    Guohua Li

  6. College of Veterinary Medicine, Northeast Agricultural University, Harbin, China

    Qiuxue Han

  7. State Key Laboratory of Agro-Biotechnology, Department of Biochemistry and Molecular Biology, College of Biological Sciences, China Agricultural University, No. 2 Yuanmingyuan West Road, Haidian District, Beijing, 100193, China

    Weiquan Liu

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  1. Nan Cheng
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Contributions

SY, YZ and WL conceived and designed the experiments. NC and WZ performed the experiments. JX, YY, HW, YG, and XX analyzed the data. NC and WZ wrote the article. GL managed and submitted the paper.

Corresponding authors

Correspondence to Songtao Yang, Weiquan Liu or Xianzhu Xia.

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Conflict of interest

The authors do not have any conflicts of interest.

Ethical approval

This study was carried out in accordance with the recommendations in the Guide for the Care and Use of Laboratory Animals developed by the China Council on Animal Care. All protocols were approved by the Committee on the Ethics of Animal Experiments of the Institute of Military Veterinary at the Academy of Military Medical Sciences. The approved ID or permit numbers were SCXK-2012-017.

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Edited by Zhen Fu.

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Cheng, N., Zhao, Y., Han, Q. et al. Development of a reverse genetics system for a feline panleukopenia virus. Virus Genes 55, 95–103 (2019). https://doi.org/10.1007/s11262-018-1621-9

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  • DOI: https://doi.org/10.1007/s11262-018-1621-9

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