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Subcellular localization of grapevine red blotch-associated virus ORFs V2 and V3

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Abstract

Grapevine red blotch-associated virus is a recently discovered plant monopartite gemini-like virus found in North American grapevines. Leaf discoloration and a decrease in fruit quality are associated with its infection. Two of its six open reading frames (ORFs), V2 and V3, are of unknown function and share no obvious homology with plant or viral genes. Transient expression of these ORFs in fusion with the green fluorescent protein demonstrated that the V2 protein localizes in the nucleoplasm, Cajal bodies, and cytoplasm; and the V3 protein localizes in various unidentified subnuclear bodies. Additionally, the V2 protein is redirected to the nucleolus upon co-expression with the nucleolus and Cajal body-associated protein Fib2.

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Acknowledgments

We would like to thank our collaborators: the Plant Cell Imaging Center and Mamta Srivastava at the Boyce Thompson Institute. For kindly providing materials, we express our gratitude to J. Burgyán (Istituto di Virologia Vegetale, CNR, Torino, Italy) for the p19 expression clone, M. Taliansky (Scottish Crop Research Institute, Invergowrie, Dundee, UK) for the AtFib2:RFP expression clone.

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Authors and Affiliations

  1. Plant Pathology and Plant–Microbe Biology Section, School of Integrative Plant Science, Cornell University, 334 Plant Science, NY, Ithaca, 14850, USA

    Tai Wei Guo, Deluxsika Vimalesvaran, Jeremy R. Thompson & Keith L. Perry

  2. Lehrstuhl für Biochemie, Department Biologie, Staudtstr. 5, 91058, Erlangen, Germany

    Björn Krenz

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  1. Tai Wei Guo
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  2. Deluxsika Vimalesvaran
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  3. Jeremy R. Thompson
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  4. Keith L. Perry
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  5. Björn Krenz
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Correspondence to Björn Krenz.

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Edited by Thomas Hohn.

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Guo, T.W., Vimalesvaran, D., Thompson, J.R. et al. Subcellular localization of grapevine red blotch-associated virus ORFs V2 and V3. Virus Genes 51, 156–158 (2015). https://doi.org/10.1007/s11262-015-1205-x

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  • DOI: https://doi.org/10.1007/s11262-015-1205-x

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