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Complete genome sequence of an isolate of Potato virus X (PVX) infecting Cape gooseberry (Physalis peruviana) in Colombia

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Abstract

Transcriptome analysis of a Cape gooseberry (Physalis peruviana) plant with leaf symptoms of a mild yellow mosaic typical of a viral disease revealed an infection with Potato virus X (PVX). The genome sequence of the PVX-Physalis isolate comprises 6435 nt and exhibits higher sequence similarity to members of the Eurasian group of PVX (~95 %) than to the American group (~77 %). Genome organization is similar to other PVX isolates with five open reading frames coding for proteins RdRp, TGBp1, TGBp2, TGBp3, and CP. 5′ and 3′ untranslated regions revealed all regulatory motifs typically found in PVX isolates. The PVX-Physalis genome is the only complete sequence available for a Potexvirus in Colombia and is a new addition to the restricted number of available sequences of PVX isolates infecting plant species different to potato.

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References

  1. G. Fischer, P.J. Almanza, D. Miranda, Rev. Bras. Frutic. 36, 1–15 (2014)

    Article  Google Scholar 

  2. G. Fischer, P.J. Almanza, Agric. Trop. 30, 79–87 (1993)

    Google Scholar 

  3. M.F. Ramadan, Food Res. Int. 44, 1830–1836 (2011)

    Article  CAS  Google Scholar 

  4. L.A. Puente, S.A. Pinto-Muñoz, E.S. Castro, M. Cortés, Food Res. Int. 44, 1733–1740 (2011)

    Article  CAS  Google Scholar 

  5. S.J. Wu, S.P. Chang, D.L. Lin, S.S. Wang, F.F. Hou, L.T. Ng, Food Chem. Toxicol. 47, 1132–1138 (2009)

    Article  CAS  PubMed  Google Scholar 

  6. C. Yen, C. Chiu, F. Chang, J. Chen, C. Hwang, Y. Hseu, H. Yang, A. Lee, M. Tsai, Z. Guo, BMC Cancer 10, 46 (2010)

    Article  PubMed Central  PubMed  Google Scholar 

  7. G.A. Garzón-Martínez, Z.I. Zhu, D. Landsman, L.S. Barrero, L. Mariño-Ramirez, BMC Genom. 13, 151 (2012)

    Article  Google Scholar 

  8. O. Prakash, A.K. Misra, S.J. Singh, K.M. Srivastava, Int. J. Trop. Plant Dis. 6, 85–87 (1988)

    Google Scholar 

  9. P. Salamon, L. Palkovics, Acta Virol. 49, 117–122 (2005)

    CAS  PubMed  Google Scholar 

  10. H.P. Trenado, I.M. Fortes, D. Louro, J. Navas-Castillo, Eur. J. Plant Pathol. 118, 193–196 (2007)

    Article  Google Scholar 

  11. M. Eiras, I.F.D. Costa, A.L.R. Chaves, A. Colariccio, New Dis. Rep. 25, 25 (2012)

    Article  Google Scholar 

  12. M. Perea, N.C. Rodriguez, G. Fischer, M. Velasquez, Y. Micán, in Biotecnología aplicada al mejoramiento de los cultivos de frutas tropicales, ed. by M. Perea, L.P. Matallana, A. Tirado (Editorial Universidad Nacional de Colombia, Bogotá, 2010), p. 466

  13. J. Zapata, A. Saldarriaga, M. Londoño, C Díaz C (Corpoica, Rionegro, 2002)

    Google Scholar 

  14. W. Aguirre-Ráquira, D. Borda, L. Hoyos-Carvajal, Potyvirus affecting Uchuva (Physalis peruviana L.) in Centro Agropecuario Marengo, Colombia. Agr. Sci. 5, 897–905 (2014)

    Google Scholar 

  15. M.G. Grabherr, B.J. Haas, M. Yassour, J.Z. Levin, D.A. Thompson, I. Amit, X. Adiconis, L. Fan, R. Raychowdhury, Q. Zeng, Z. Chen, E. Mauceli, N. Hacohen, A. Gnirke, N. Rhind, F. di Palma, B.W. Birren, C. Nusbaum, K. Lindblad-Toh, N. Friedman, A. Regev, Nat. Biotechnol. 29, 644–652 (2011)

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  16. B. Langmead, S. Salzberg, Nat. Methods 9, 357–359 (2012)

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  17. W. Gish, D.J. States, Nat. Genet. 3, 266–272 (1993)

    Article  CAS  PubMed  Google Scholar 

  18. K. Tamura, G. Stecher, D. Peterson, A. Filipski, S. Kumar, Mol. Biol. Evol. 30, 2725–2729 (2013)

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  19. D. Kutnjak, R. Silvestre, W. Cuellar, W. Perez, G. Müller, M. Ravnikar, J. Kreuze, Virus Res. 191, 45–50 (2014)

    Article  CAS  PubMed  Google Scholar 

  20. J. Verchot-Lubicz, C.M. Ye, D. Bamunusinghe, J. Gen. Virol. 88, 1643–1655 (2007)

    Article  CAS  PubMed  Google Scholar 

  21. K.H. Kim, C.L. Hemenway, J. Virol. 70, 5533–5540 (1996)

    PubMed Central  CAS  PubMed  Google Scholar 

  22. J.S. Batten, S. Yoshinari, C. Hemenway, Mol. Plant Pathol. 4, 125–131 (2003)

    Article  CAS  PubMed  Google Scholar 

  23. V.S. Sriskanda, G. Pruss, X. Ge, V.B. Vance, J. Virol. 70, 5266–5271 (1996)

    PubMed Central  CAS  PubMed  Google Scholar 

  24. N. Pillai-Nair, K.H. Kim, C. Hemenway, J. Mol. Biol. 326, 701–720 (2003)

    Article  CAS  PubMed  Google Scholar 

  25. A. Lange, R.E. Mills, C.J. Lange, M. Stewart, S.E. Devine, A.H. Corbett, J. Biol. Chem. 282, 5101–5105 (2007)

    Article  CAS  PubMed  Google Scholar 

  26. D. Bamunusinghe, C.L. Hemenway, R.S. Nelson, A.A. Sanderfoot, C.M. Ye, M.A. Silva, M. Payton, J. Verchot-Lubicz, Virology 393, 272–285 (2009)

    Article  CAS  PubMed  Google Scholar 

  27. A.R. Howard, M.L. Heppler, H.J. Ju, K. Krishnamurthy, M.E. Payton, J. Verchot-Lubicz, Virology 328, 185–197 (2004)

    Article  CAS  PubMed  Google Scholar 

  28. I. Malcuit, M.R. Marano, T.A. Kavanagh, W. De Jong, A. Forsyth, D.C. Baulcombe, Mol. Plant Microbe Interact. 12, 536–543 (1999)

    Article  CAS  Google Scholar 

  29. R. Mitra, K. Krishnamurthy, E. Blancaflor, M. Payton, R.S. Nelson, J. Verchot-Lubicz, Virology 312, 35–48 (2003)

    Article  CAS  PubMed  Google Scholar 

  30. H.J. Ju, C.M. Ye, J. Verchot-Lubicz, Virology 375, 103–117 (2008)

    Article  CAS  PubMed  Google Scholar 

  31. S. Santa Cruz, D. Baulcombe, Analysis of potato virus X coat protein genes in relation to resistance conferred by the genes Nx, Nb and Rx1 of potato. J. Gen. Virol. 76, 2057–2061 (1995)

    Article  CAS  PubMed  Google Scholar 

  32. N. Esfandiari, M. Kohi-Habibi, G. Mosahebi, Commun. Agric. Appl. Biol. Sci. 71, 1281–1287 (2006)

    CAS  PubMed  Google Scholar 

  33. B.A. Cox, R.A.C. Jones, Arch. Virol. 155, 1349–1356 (2010)

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

This work was funded by Universidad Nacional de Colombia through Grant VRI: 19438 and International Foundation for Science (IFS, Sweden, Research Grant Agreement NO. C/4634-2).

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The authors declare that they have no conflict of interest.

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Correspondence to Pablo A. Gutiérrez.

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Edited by Seung-Kook Choi.

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Gutiérrez, P.A., Alzate, J.F. & Montoya, M.M. Complete genome sequence of an isolate of Potato virus X (PVX) infecting Cape gooseberry (Physalis peruviana) in Colombia. Virus Genes 50, 518–522 (2015). https://doi.org/10.1007/s11262-015-1181-1

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