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Molecular characteristics of the complete genome of a J-subgroup avian leukosis virus strain isolated from Eurasian teal in China

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Abstract

The J-subgroup avian leukosis virus (ALV-J) strain WB11098J was isolated from a wild Eurasian teal, and its proviral genomic sequences were determined. The complete proviral sequence of WB11098J was 7868 nt long. WB11098J was 95.3.9 % identical to the prototype strain HPRS-103, 94.2 % identical to the American strain ADOL-7501, 94.5–94.7 % identical to Chinese broiler isolates, 94.8–97.5 % identical to layer chicken isolates, and 94.4–95.0 % identical to Chinese local chicken isolates at the nucleotide level. Phylogenetic analysis showed that the WB11098J isolate shared the greatest homology with the layer strain SD09DP03 and was included in the same cluster. Interestingly, two 19-bp insertions in the U3 regions of the 5′LTR and 5′UTR that were most likely derived from other retroviruses were found in the WB11098J isolate. These insertions separately introduced one E2BP-binding site in the U3 region of the 5′LTR and a RNA polymerase II transcription factor IIB and core promoter motif of ten elements in the 5′UTR. A 5-bp deletion was identified in the U3 region of the 5′LTR. No nucleotides were deleted in the rTM or DR-1 regions in the 3′UTR. A 1-bp deletion was detected in the E element and introduced a specific and distinct binding site for c-Ets-1. Our study is the first to report the molecular characteristics of the complete genome of an ALV-J that was isolated from a wild bird and will provide necessary information for further understanding of the evolution of ALV-J.

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Acknowledgments

The study was supported by the Fundamental Research Funds for the Central Universities (DL12CA14), the State Key Laboratory of Veterinary Biotechnology Foundation (SKLVBF201201), the Special Fund for Agro-scientific Research in the Public Interest (No. 201203055), the Natural Science Foundation of the Heilongjiang Province of China (C201223), and Programs of the State Forestry Administration of Wildlife Protection and Nature Reserve Management Division (2013).

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The authors declare that they have no competing interests.

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Authors and Affiliations

  1. College of Wildlife Resources, Northeast Forestry University, Harbin, 150040, China

    Xiangwei Zeng, Ruijun Hao, Wansi Liu & Chunyan Han

  2. Division of Avian Infectious Diseases, National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute Chinese Academy of Agricultural Sciences, No. 427 Maduan St., Harbin, 150001, China

    Xiangwei Zeng, Yulong Gao, Delong Li, Ruijun Hao, Wansi Liu, Chunyan Han, Honglei Gao, Xiaole Qi, Yongqiang Wang & Xiaomei Wang

  3. College of Basic Medical Science, Heilongjiang University of Chinese Medicine, Harbin, 150040, China

    Lanlan Liu

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  1. Xiangwei Zeng
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Correspondence to Xiaomei Wang.

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Xiangwei Zeng and Yulong Gao contributed equally to this paper.

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Zeng, X., Gao, Y., Li, D. et al. Molecular characteristics of the complete genome of a J-subgroup avian leukosis virus strain isolated from Eurasian teal in China. Virus Genes 49, 250–258 (2014). https://doi.org/10.1007/s11262-014-1081-9

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