Skip to main content

Advertisement

SpringerLink
Log in

The C2 protein of Bhendi yellow vein mosaic virus plays an important role in symptom determination and virus replication

  • Published:
Virus Genes Aims and scope Submit manuscript

Abstract

Bhendi yellow vein mosaic virus (BYVMV) that causes bhendi yellow vein mosaic disease is a monopartite begomovirus with an associated betasatellite. Previous studies have shown that C2 protein of BYVMV acts as a suppressor of post transcriptional gene silencing, activates transcription, localizes to nucleus, and interacts with karyopherin α. To probe the role of C2 in symptom determination and virus replication, the infectious clones of BYVMV containing two stop codons in the C2 ORF were created and used for infection studies. The Nicotiana benthamiana plants infiltrated with the infectious clones harboring stop codons in the C2 ORF did not develop any symptoms unlike plants infiltrated with wild-type BYVMV. Southern blotting and real time PCR analysis revealed that the viral load was reduced drastically in the plants infected with BYVMV containing the nontranslatable version of C2 ORF. However, there was a recovery in viral DNA replication, when co-infiltrated with wild-type betasatellite. Hence we conclude that the C2 protein of BYVMV plays an important role in symptom determination and viral DNA replication.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

References

  1. S. Baliji, J. Sunter, G. Sunter, Mol. Plant Microbe Interact. 20, 194–206 (2007)

    Article  CAS  PubMed  Google Scholar 

  2. S.A. Chandran, Y. Levy, A. Mett, E. Belausov, U. Ramakrishnan, Y. Gafni, J. Gen. Virol. 93, 1367–1374 (2012)

    Article  CAS  PubMed  Google Scholar 

  3. D.H. Figurski, D.R. Helinski, Proc Natl Acad Sci U S A 76, 1648–1652 (1979)

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  4. P. Gopal, P. Pravin Kumar, B. Sinilal, J. Jose, A. Kasin Yadunandam, R. Usha, Virus Res. 123, 9–18 (2007)

    Article  CAS  PubMed  Google Scholar 

  5. P. Hajdukiewicz, Z. Svab, P. Maliga, Plant Mol. Biol. 25, 989–994 (1994)

    Article  CAS  PubMed  Google Scholar 

  6. Y. Hong, J. Stanley, Mol. Plant Microbe Interact. 4, 219–225 (1996)

    Article  Google Scholar 

  7. J. Jose, R. Usha, Virology 305, 310–317 (2003)

    Article  CAS  PubMed  Google Scholar 

  8. S.G. Lazarowitz, Crit. Rev. Plant Sci. 11, 327–349 (1992)

    CAS  Google Scholar 

  9. S. Mansoor, R.W. Briddon, Y. Zafar, J. Stanley, Trends Plant Sci. 8, 128–134 (2003)

    Article  CAS  PubMed  Google Scholar 

  10. S.O. Rogers, A.J. Bendich, Plant Molecular Biology Manual, D 1 edn. (Kluwer Academic Publishers, Boston, 1994), pp. 1–8

    Google Scholar 

  11. M.R. Rojas, C. Hagen, W.J. Lucas, R.L. Gilbertson, Annu. Rev. Phytopathol. 43, 361–394 (2005)

    Article  CAS  PubMed  Google Scholar 

  12. J. Stanley, J.R. Latham, M.S. Pinner, I. Bedford, P.G. Markham, Virology 191, 396–405 (1992)

    Article  CAS  PubMed  Google Scholar 

  13. J. Vieira, J. Messing, Gene 100, 189–194 (1991)

    Article  CAS  PubMed  Google Scholar 

  14. Z. Zhang, H. Chen, X. Huang, R. Xia, Q. Zhao, J. Lai, K. Teng, Y. Li, L. Liang, Q. Du, X. Zhou, H. Guo, Q. Xie, Plant Cell 23, 273–288 (2011)

    Article  PubMed Central  PubMed  Google Scholar 

Download references

Acknowledgments

The authors thank the Department of Biotechnology, Government of India for funding and fellowships to S.A.C and C.J. The authors are grateful to Dr. Y. Gafni and Dr. P. Gopal for useful discussions. The authors also thank Dr. K. Veluthambi and Dr. V. G. Malathi for providing pRK2013 and EHA105, respectively.

Author information

Authors and Affiliations

  1. Department of Plant Biotechnology, School of Biotechnology, Madurai Kamaraj University, Madurai, India

    Sam A. Chandran, C. Jeyabharathy & R. Usha

Authors
  1. Sam A. Chandran
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. C. Jeyabharathy
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. R. Usha
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to R. Usha.

Additional information

Sam A. Chandran and C. Jeyabharathy have contributed equally.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Chandran, S.A., Jeyabharathy, C. & Usha, R. The C2 protein of Bhendi yellow vein mosaic virus plays an important role in symptom determination and virus replication. Virus Genes 48, 203–207 (2014). https://doi.org/10.1007/s11262-013-0992-1

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11262-013-0992-1

Keywords

Search

Navigation