Skip to main content
SpringerLink
Log in

Infectivity analysis of a blackgram isolate of Mungbean yellow mosaic virus and genetic assortment with MYMIV in selective hosts

  • Published:
Virus Genes Aims and scope Submit manuscript

Abstract

Yellow mosaic disease in grain legumes in Indian subcontinent is caused by two important virus species viz. Mungbean yellow mosaic virus (MYMV) and Mungbean yellow mosaic India virus (MYMIV), belonging to the genus Begomovirus of the family Geminiviridae. The genomic components of a begomovirus causing yellow mosaic disease in blackgram in southern India were cloned and sequenced. Nucleotide sequence comparison of DNA A component shows the virus isolate to be a variant of Mungbean yellow mosaic virus:–(MYMV-[IN:Vam:05]). However, DNA B component of the present virus isolate has greater similarity (92%) to Mungbean yellow mosaic India virus. Agroinoculations of the viral clones produced typical yellow mosaic symptoms in blackgram and mungbean, severe leaf curl and stunting in French bean, similar to blackgram isolate of MYMIV. Blackgram isolates of both the virus species were only mildly infectious on cowpea, produced atypical leaf curl symptoms and not yellow or golden mosaic. In agroinoculations done by exchanging genomic components, symptom expression was seen only in French bean. In cowpea, blackgram and mungbean there was no visible symptoms though viral DNA could be detected by PCR.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  1. A. Varma, A.K. Dhar, B. Mandal, in Mungbean yellow mosaic diseases, ed. by S.K. Green, D. Kin (Asian Vegetable Research and Development Centre, Taipei, 1992), pp. 8–27

    Google Scholar 

  2. A. Varma, V.G. Malathi, Ann. Appl. Biol. 142, 145–164 (2003)

    Article  CAS  Google Scholar 

  3. V.G. Malathi, P. John, Mungbean yellow mosaic viruses, in Encyclopedia of virology, vol. 8, ed. by B.W.J. Mahy, M.H.V. Van Regenmortel (Elsevier Ltd, Amsterdam, 2008), pp. 364–371

    Chapter  Google Scholar 

  4. K.S. Usharani, B. Surendranath, Q.M.R. Haq, V.G. Malathi, Curr. Sci. 86, 845–850 (2004)

    CAS  Google Scholar 

  5. A.S. Karthikeyan, R. Vanitharani, V. Balaji, S. Anuradha, P. Thillaichidambaram, P.V. Shivaprasad, C. Parameswari, V. Balamani, M. Saminathan, K. Veluthambi, Arch. Virol. 149, 1643–1652 (2004)

    Article  CAS  PubMed Central  Google Scholar 

  6. K.R. Girish, R. Usha, Virus Res. 108, 167–176 (2005)

    Article  CAS  PubMed Central  Google Scholar 

  7. L. Hanley-Bowdoin, S.B. Settlage, B.M. Orozco, S. Nagar, D. Robertson, Crit. Rev. Plant Sci. 18, 71–106 (1999)

    Article  CAS  Google Scholar 

  8. P. John, P.N. Sivalingam, Q.M.I. Haq, N. Kumar, A. Mishra, R.W. Briddon, V.G. Malathi, Arch. Virol. 153, 1359–1365 (2008)

    Article  CAS  PubMed Central  Google Scholar 

  9. V. Balaji, R. Vanitharani, A.S. Karthikeyan, S. Anbalagan, K. Veluthambi, J. Biosci. 29, 297–308 (2004)

    Article  CAS  PubMed Central  Google Scholar 

  10. A. Rouhibakhsh, J. Priya, M. Periasamy, Q.M.I. Haq, V.G. Malathi, J. Virol. Methods 147, 37–42 (2008)

    Article  CAS  PubMed Central  Google Scholar 

  11. J. Qazi, M. Ilyas, S. Mansoor, R.W. Briddon, Mol. Plant Pathol. 8, 343–348 (2007)

    Article  PubMed Central  Google Scholar 

  12. J. Sambrook, D.W. Russel, Molecular cloning, a laboratory manual, 3rd edn. (Cold Spring Harbor Laboratory Press, New York, 2001)

    Google Scholar 

  13. T.A. Hall, Nucl. Acids Symp. Ser. 41, 95–98 (1999)

    CAS  Google Scholar 

  14. C.M. Fauquet, R.W. Briddon, J.K. Brown, E. Moriones, J. Stanley, M. Zerbini, X. Zhou, Arch. Virol. 153, 783–821 (2008)

    Article  CAS  PubMed Central  Google Scholar 

  15. K. Tamura, J. Dudley, M. Nei, S. Kumar, Mol. Biol. Evol. 24, 1596–1599 (2007)

    Article  CAS  Google Scholar 

  16. B. Surendranath, K.S. Usharani, A. Nagma, A.K. Victoria, V.G. Malathi, Arch. Virol. 150, 1833–1844 (2005)

    Article  CAS  PubMed Central  Google Scholar 

  17. E.E. Hood, S.B. Gelvin, S. Melchers, A. Hoekema, Transgenic Res. 2, 208–218 (1993)

    Article  CAS  Google Scholar 

  18. G. Ditta, S. Stanfield, D. Corbin, O.R. Helinksi, Proc. Natl Acad. Sci. USA 77, 7347–7351 (1080)

    Article  Google Scholar 

  19. B. Mandal, A. Varma, V.G. Malathi, J. Phytopathol. 145, 503–510 (1997)

    Article  Google Scholar 

  20. G.R. Arguello-Astorga, R.G. Guenara-Gonzalez, L.R. Herrera-Estrella, R.F. Rivera-Bustamante, Virology 203, 90–100 (1994)

    Article  CAS  PubMed Central  Google Scholar 

  21. M. Ilyas, J. Qazi, S. Mansoor, R.W. Briddon, Virus Res. 145, 279–284 (2009)

    Article  CAS  PubMed Central  Google Scholar 

  22. R.W. Briddon, B.L. Patil, B. Bagewadi, M.S. Nawaz-ul-Rehman, C.M. Fauquet, BMC Evol. Biol. 10, 97 (2010)

    Article  PubMed Central  Google Scholar 

  23. K.K. Biswas, A. Varma, Indian Phytopathol. 53, 134–141 (2000)

    CAS  Google Scholar 

  24. V.G. Malathi, B. Surendranath, A. Naghma, A. Roy, Can. J. Plant Pathol. 27, 439–447 (2005)

    Article  CAS  Google Scholar 

  25. K.S. Usharani, V. Balaji, B. Surendranath, Q.M.R. Haq, V.G. Malathi, J. Gen. Plant. Pathol. 17, 230–237 (2005)

    Article  Google Scholar 

  26. S.S. Jacob, Vanitha Rani, A.S. Karthikeyan, Y. Chinchore, P. Thillaichidambaram, K. Veluthambi, Plant Dis. 87, 247–251 (2003)

    Article  CAS  PubMed Central  Google Scholar 

Download references

Acknowledgments

The financial support given by Indian Council of Agricultural Research (ICAR network project on transgenic in crops, cotton and soybean). Government of India is gratefully acknowledged. We are thankful to Dr. H.S.Gupta, Director, Indian Agricultural Research Institute, New Delhi and Dr.R.K.Jain, Head, division of Plant Pathology, Indian Agricultural Research Institute, New Delhi-110012, India, for providing necessary facilities. We are also grateful to the scientists and staff at National Phytotron Facility, Indian Agricultural Research Institute, India for their guidance and support to grow plants under controlled conditions.

Author information

Authors and Affiliations

  1. Plant Molecular Biology Group, International Centre for Genetic Engineering and Biotechnology, New Delhi, 110067, India

    Q. M. I. Haq

  2. Division of Plant Pathology, Agricultural Faculty, Gilan University, Rasht, Iran

    A. Rouhibakhsh

  3. Department of Biotechnology, Jamia Millia Islamia (A Central University), New Delhi, 110025, India

    Arif Ali

  4. Advanced Centre for Plant Virology, Division of Plant Pathology, Indian Agricultural Research Institute, New Delhi, 110012, India

    V. G. Malathi

Authors
  1. Q. M. I. Haq
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. A. Rouhibakhsh
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Arif Ali
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. V. G. Malathi
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to V. G. Malathi.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Haq, Q.M.I., Rouhibakhsh, A., Ali, A. et al. Infectivity analysis of a blackgram isolate of Mungbean yellow mosaic virus and genetic assortment with MYMIV in selective hosts. Virus Genes 42, 429–439 (2011). https://doi.org/10.1007/s11262-011-0591-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11262-011-0591-y

Keywords

Search

Navigation