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Activation of NF-κB by nucleocapsid protein of the porcine reproductive and respiratory syndrome virus

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Abstract

Nuclear factor kappa B (NF-κB) is a critical transcription factor in innate and adaptive immune response as well as cell proliferation and survival. Previous studies have demonstrated that porcine reproductive and respiratory syndrome virus (PRRSV) infection activated NF-κB pathways through IκB degradation in MARC-145 cells and alveolar macrophages. To evaluate the mechanisms behind this, we investigated the role of PRRSV structural proteins in the regulation of NF-κB. In this study, we screened the structural proteins of PRRSV by NF-κB DNA-binding assay and luciferase activity assay and demonstrated that PRRSV nucleocapsid (N) protein could activate NF-κB in MARC-145 cells. Furthermore, we revealed that the region between aa 30 and 73 of N protein was essential for its function in the activation of NF-κB. These results presented here provide a basis for understanding molecular mechanism of PRRSV infection and inflammation response.

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Acknowledgments

This work was supported by the National Natural Sciences Foundation of China (31001054, 30800046) and the New Century Excellent Talent Project (NCET-07-0347).

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Authors and Affiliations

  1. State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, 430070, China

    Rui Luo, Liurong Fang, Yunbo Jiang, Hui Jin, Yanwei Wang, Dang Wang, Huanchun Chen & Shaobo Xiao

  2. Laboratory of Animal Virology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, China

    Rui Luo, Liurong Fang, Yunbo Jiang, Hui Jin, Yanwei Wang, Dang Wang, Huanchun Chen & Shaobo Xiao

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  1. Rui Luo
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  2. Liurong Fang
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  8. Shaobo Xiao
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Correspondence to Shaobo Xiao.

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Luo, R., Fang, L., Jiang, Y. et al. Activation of NF-κB by nucleocapsid protein of the porcine reproductive and respiratory syndrome virus. Virus Genes 42, 76–81 (2011). https://doi.org/10.1007/s11262-010-0548-6

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  • DOI: https://doi.org/10.1007/s11262-010-0548-6

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