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Quasispecies nature of Pepino mosaic virus and its evolutionary dynamics

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Abstract

Genetic variability is an essential feature of RNA viruses. It allows them to adapt to the ever-changing environmental conditions. Important biological properties of the viruses, their infectivity, adaptability, and host range, may also depend on the level of quasispecies diversity. Here, we present the analysis of the genetic polymorphism of Pepino mosaic virus (PepMV). The examined populations were isolated from the naturally infected tomato plants (Solanum lycopersicum). In order to determine the complexity of the PepMV populations, the number of different viral variants and their genetic diversity was established. Moreover, phylogenetic trees were created to depict relations between the identified variants. For the first time we have shown that the PepMV exists as a quasispecies. The observed level of genetic variability allows PepMV for a quick and flexible adaptation to different hosts. Our results suggest that the level of PepMV variability possibly influences the course of infection.

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References

  1. J.W. Drake, Proc. Natl. Acad. Sci. USA 90, 4171–4175 (1993)

    Article  CAS  PubMed  Google Scholar 

  2. J.W. Drake, J.J. Holland, Proc. Natl. Acad. Sci. USA 96(24), 13910–13913 (1999)

    Article  CAS  PubMed  Google Scholar 

  3. D.A. Steinhauer, E. Domingo, J.J. Holland, Gene 122, 281–288 (1992)

    Article  CAS  PubMed  Google Scholar 

  4. B. Tippin, P. Phuong, F. Myron, M.F. Goodman, Trends Microbiol. 12, 288–295 (2004)

    Article  CAS  PubMed  Google Scholar 

  5. P. Naraghi-Arani, S. Daubert, A. Rowhani, J. Gen. Virol. 82, 1791–1795 (2001)

    CAS  PubMed  Google Scholar 

  6. W.L. Schneider, M.J. Roossinck, J. Virol. 75, 6566–6571 (2001)

    Article  CAS  PubMed  Google Scholar 

  7. J.P. Legg, J.M. Thresh, Virus Res. 71, 135–149 (2000)

    Article  CAS  PubMed  Google Scholar 

  8. W.L. Schneider, M.J. Roossinck, J. Virol. 74, 3130–3134 (2000)

    Article  CAS  PubMed  Google Scholar 

  9. J.M. Aguilar, M.D. Hernandez-Gallarod, J.L. Cenis, A. Lacasa, M.A. Aranda, Arch. Virol. 147, 2009–2015 (2002)

    Article  CAS  PubMed  Google Scholar 

  10. S.Y. Morozov, A.G.J. Solovyev, Gen. Virol. 84, 1351–1366 (2003)

    Article  CAS  Google Scholar 

  11. T.J. Lough, S.J. Emerson, W.J. Lucas, R.L.S. Forster, Virology 288, 18–28 (2001)

    Article  CAS  PubMed  Google Scholar 

  12. S.S. Cruz, A.G. Roberts, D.A.M. Prior, S. Chapman, K.J. Oparka, Plant Cell 10, 495–510 (1998)

    Article  CAS  PubMed  Google Scholar 

  13. A.C. Cotillon, M. Girard, S. Ducouret, Arch. Virol. 147, 2231–2238 (2002)

    Article  CAS  PubMed  Google Scholar 

  14. I. Pagan, M.C. Córdoba-Sellés, L. Martínez-Priego, A. Fraile, J.M. Malpica, C. Jordá, F. García-Arenal, Phytopathology 96, 274–279 (2006)

    Article  CAS  PubMed  Google Scholar 

  15. K.S. Ling, Virus Genes 34, 1–8 (2007)

    Article  CAS  PubMed  Google Scholar 

  16. I.M. Hanssen, A. Paeleman, L. Wittemans, K. Goen, B. Lievens, C. Bragard, A.C.R.C. Vanachter, B.P.H.J. Thomma, Eur. J. Plant Pathol. 121, 131–146 (2008)

    Article  Google Scholar 

  17. B. Hasiów, N. Borodynko, H. Pospieszny, Virus Genes 36, 209–214 (2008)

    Article  PubMed  Google Scholar 

  18. P. Gómez, R.N. Sempere, S.F. Elena, M.A. Aranda, International Conference Genetic Control of Plant Pathogenic Viruses and Their Vectors: Towards New Resistance Strategies, Puerto de Santa Maria, Spain, Book of abstracts, 70, 2008

  19. C.J. French, A. Bunckle, G. Ferguson, C. Dubeau, M Bouthillier, M.G. Bernardy, J. Plant Pathol. 90(Suppl), s2.198 (2008)

    Google Scholar 

  20. K.S. Ling, Plant Dis. 92, 1683–1688 (2008)

    Article  CAS  Google Scholar 

  21. I.M. Hanssen, B.P.H.J. Thomma, Mol Plant Pathol 11(2), 179–182 (2010)

    Article  CAS  PubMed  Google Scholar 

  22. P. Kędziora, M. Figlerowicz, P. Formanowicz, M. Alejska, P. Jackowiak, N. Malinowska, A. Frątczak, J. Błażewicz, M. Figlerowicz, Bull. Pol. Acad. Sci. 53, 273–281 (2005)

    Article  Google Scholar 

  23. J.D. Thompson, D.G. Higgins, T.J. Gibson, Nucleic Acids Res. 22, 4673–4680 (1994)

    Article  CAS  PubMed  Google Scholar 

  24. S. Kumar, K. Tamura, M. Nei, MEGA3: I. Brief. Bioinform. 5, 150–163 (2004)

    Article  CAS  Google Scholar 

  25. R. Feuer, J.D. Boone, D. Netski, S.P. Morzunov, S.C. St. Jeor, J. Virol. 73, 9544–9554 (1999)

    CAS  PubMed  Google Scholar 

  26. A. Alfaro-Fernández, G. Bese, M.C. Córdoba-Sellés, M.C. Cebrain, J.A. Herrera-Vásquez, A. Forray, C. Jordá, Plant Dis. 93, 554 (2009)

    Article  Google Scholar 

  27. M. Verbeek, A.M. Dullemans, J.F.J. Van der Heuvel, P.C. Maris, R.A.A. Van der Vlugt, Arch. Virol. 152, 881–990 (2007)

    Article  CAS  PubMed  Google Scholar 

  28. E.R. Chare, E.C. Holmes, J. Gen. Virol. 85, 3149–3157 (2004)

    Article  CAS  PubMed  Google Scholar 

  29. B.T. Grenfell, O.G. Pybus, J.R. Gog, J.L.N. Wood, J.M. Daly, J.A. Mumford, E.C. Holmes, Science 303, 327–332 (2004)

    Article  CAS  PubMed  Google Scholar 

  30. R. French, D.C. Stenger, Annu. Rev. Phytopathol. 41, 335–342 (2003)

    Article  Google Scholar 

  31. H. Li, M.J. Roossinck, J. Virol. 78, 10582–10587 (2004)

    Article  CAS  PubMed  Google Scholar 

  32. C. Marco, M.A. Aranda, J. Gen. Virol. 86, 815–822 (2005)

    Article  CAS  PubMed  Google Scholar 

  33. H. Senshu, J. Ozeki, K. Komatsu, M. Hashimoto, K. Hatada, M. Aoyama, S. Kagiwada, Y. Yamaji, S. Namba, J. Gen. Virol. 90, 1014–1024 (2009)

    Article  CAS  PubMed  Google Scholar 

  34. N.S. Al-Kaff, S.N. Covey, M.M. Kreike, A.M. Page, R. Pinder, P.J. Dale, Science 279, 2113–2115 (1998)

    Article  CAS  PubMed  Google Scholar 

  35. A.J. Hamilton, D.C. Baulcombe, Science 286, 950–952 (1999)

    Article  CAS  PubMed  Google Scholar 

  36. O. Voinnet, C. Lederer, D.C. Baulcombe, Cell 103, 157–167 (2000)

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgment

This work was supported by grant N N310 163 438 from the Polish Ministry of Science and Higher Education.

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Authors and Affiliations

  1. Department of Virology and Bacteriology, Institute of Plant Protection-National Research Institute, ul. Węgorka 20, 60-318, Poznań, Poland

    Beata Hasiów-Jaroszewska, Natasza Borodynko & Henryk Pospieszny

  2. Institute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego 12/14, 61-704, Poznań, Poland

    Paulina Jackowiak & Marek Figlerowicz

Authors
  1. Beata Hasiów-Jaroszewska
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  2. Paulina Jackowiak
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  3. Natasza Borodynko
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  5. Henryk Pospieszny
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Correspondence to Beata Hasiów-Jaroszewska.

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Hasiów-Jaroszewska, B., Jackowiak, P., Borodynko, N. et al. Quasispecies nature of Pepino mosaic virus and its evolutionary dynamics. Virus Genes 41, 260–267 (2010). https://doi.org/10.1007/s11262-010-0497-0

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  • DOI: https://doi.org/10.1007/s11262-010-0497-0

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