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Misleading results of the MboII-based identification of type 2a canine parvovirus strains from Hungary reacting as type 2c strains

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Abstract

Type 2 canine parvovirus (CPV2) infection is one of the most frequent causes of death in the young, susceptible canine populations worldwide. Since its emergence in the 1970s, several variants have been described. In the present study the authors describe the genetic analysis of 24 Hungarian CPV2 strains collected from 2004 to 2008. Surprisingly, the genetic and phylogenetic investigations of all these strains revealed that all of them were type 2a CPVs. On the other hand, the genetic analysis provided substantial evidence to demonstrate that due to a seemingly constant point mutation present in most of the Hungarian CPV2a strains, a previously described MboII-based rapid identification of CPV2c strains unfortunately cannot be reliably used any more.

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References

  1. W.R. Kelly, Aust. Vet. J. 54, 593 (1978)

    Article  CAS  PubMed  Google Scholar 

  2. M.J.G. Appel, F.W. Scott, L.E. Carmichael, Vet. Rec. 105, 156 (1979)

    CAS  PubMed  Google Scholar 

  3. G. Burtonboy, F. Coignoul, N. Delferriere, P.P. Pastoret, Arch. Virol. 61, 1 (1979)

    Article  CAS  PubMed  Google Scholar 

  4. R.H. Johnson, P.B. Spradbrow, Aust. Vet. J. 55, 151 (1979)

    Article  Google Scholar 

  5. G. Koptopoulos, O. Papadopoulos, M. Papnastastasopoulou, H.J.C. Cornwell, Vet. Rec. 118, 332 (1986)

    CAS  PubMed  Google Scholar 

  6. L.A. Shackelton, C.R. Parrish, U. Truyen, E.C. Holmes, Proc. Natl. Acad. Sci. USA 11, 379 (2005)

    Article  Google Scholar 

  7. J. Hetzl, P.R. Tomsich, B. Etschmann, C.R. Parrish, P.F. Stadler, U. Truyen, in Abstracts of the International Parvovirus Symposium, International Parvovirus Symposium, Leipzig, Germany (2005)

  8. U. Truyen, Vet. Microbiol. 117, 9 (2006)

    Article  CAS  PubMed  Google Scholar 

  9. C.R. Parrish, P.H. O’Connell, J.F. Evermann, L.E. Carmichael, Science 230, 1046 (1985)

    Article  CAS  PubMed  Google Scholar 

  10. P. Tijssen, Acta Vet. Hung. 47, 379 (1999)

    Article  CAS  PubMed  Google Scholar 

  11. C.R. Parrish, C.F. Aquadro, M.L. Strassheim, J.F. Evermann, J.Y. Sgro, H.O. Mohammed, J. Virol. 65, 6544 (1991)

    CAS  PubMed  Google Scholar 

  12. M. Mochizuki, R. Harasawa, H. Nakatani, Vet. Microbiol. 38, 1 (1993)

    Article  CAS  PubMed  Google Scholar 

  13. C.R. Parrish, Semin. Virol 5, 121 (1994)

    Article  Google Scholar 

  14. R.R. de Ybanez, C. Vela, E. Cortes, I. Simarro, J.I. Casal, Vet. Rec. 136, 174 (1995)

    PubMed  Google Scholar 

  15. N.M. Greenwood, W.S.K. Chalmers, W. Baxendale, H. Thompson, Vet. Rec. 138, 495 (1996)

    CAS  PubMed  Google Scholar 

  16. U. Truyen, G. Platzer, C.R. Parrish, Vet. Rec. 138, 365 (1996)

    CAS  PubMed  Google Scholar 

  17. U. Truyen, A. Steinel, L. Bruckner, H. Lutz, K. Mostl, Schweizer Archiv für Tierheilkunde 142, 115 (2000)

    CAS  PubMed  Google Scholar 

  18. A. Steinel, E.H. Venter, M. Van Vuuren, C.R. Parrish, U. Truyen, Onderstepoort J. Vet. Res. 65, 239 (1998)

    CAS  PubMed  Google Scholar 

  19. F. Bíró, K. Vörös, V. Pálfi, V.L. Papp, Magyar Állatorvosok 121, 248 (1999)

    Google Scholar 

  20. D. Buonavoglia, A. Cavalli, A. Pratelli, V. Martella, G. Greco, M. Tempesta, C. Buonavoglia, New Microbiol. 23, 93 (2000)

    CAS  PubMed  Google Scholar 

  21. C. Buonavoglia, V. Martella, A. Pratelli, M. Tempesta, A. Cavalli, D. Buonavoglia, G. Bozzo, G. Elia, N. Decaro, L.E. Carmichael, J. Gen. Virol. 82, 3021 (2001)

    CAS  PubMed  Google Scholar 

  22. C.A. Pereira, T.A. Monezi, D.U. Mehnert, M. D’Angelo, E.L. Durigon, Vet. Microbiol. 75, 127 (2000)

    Article  CAS  PubMed  Google Scholar 

  23. N. Decaro, V. Martella, C. Desario, A.L. Bellacicco, M. Camero, L. Manna, D. d’Aloja, C. Buonavoglia, J. Vet. Med. B Infect Dis. Vet. Public Health 53, 468 (2006)

    CAS  PubMed  Google Scholar 

  24. N. Decaro, C. Desario, G. Elia, M. Campolo, A. Lorusso, V. Mari, V. Martella, C. Buonavoglia, Vaccine 25, 1161 (2007)

    Article  CAS  PubMed  Google Scholar 

  25. M. Nakamura, Y. Tohya, T. Miyazawa, M. Mochizuki, H.T. Phung, N.H. Nguyen, L.M. Huynh, L.T. Nguyen, P.N. Nguyen, P.V. Nguyen, N.P. Nguyen, H. Akashi, Arch. Virol. 149, 2261 (2004)

    Article  CAS  PubMed  Google Scholar 

  26. S. Nandi, S. Chidri, M. Kumar, R.S. Chauhan, Res. Vet. Sci. 88, 169 (2010)

    Article  CAS  PubMed  Google Scholar 

  27. R. Pérez, L. Francia, V. Romero, L. Maya, I. López, M. Hernández, Vet. Microbiol. 124, 147 (2007)

    Article  PubMed  Google Scholar 

  28. C. Hong, N. Decaro, C. Desario, P. Tanner, M.C. Pardo, S. Sanchez, C. Buonavoglia, J.T. Saliki, J. Vet. Diagn. Investig. 19, 535 (2007)

    Google Scholar 

  29. L. Touihri, I. Bouzid, R. Daoud, C. Desario, A.F. El Goulli, N. Decaro, A. Ghorbel, C. Buonavoglia, C. Bahloul, Virus Genes 38, 249 (2009)

    Article  CAS  PubMed  Google Scholar 

  30. Y. Ikeda, K. Nakamura, T. Miyazawa, Y. Tohya, E. Takahashi, M. Mochizuki, Emerg. Infect. Dis. 8, 341 (2002)

    Article  PubMed  Google Scholar 

  31. V. Martella, A. Cavalli, A. Pratelli, G. Bozzo, M. Camero, D. Buonavoglia, D. Narcisi, M. Tempesta, C. Buonavoglia, J. Clin. Microbiol. 42, 1333 (2004)

    Article  PubMed  Google Scholar 

  32. N. Decaro, G. Elia, M. Campolo, C. Desario, M.S. Lucente, A.L. Bellacicco, C. Buonavoglia, J. Vet. Med. B Infect. Dis. Vet. Public Health 52, 316 (2005)

    CAS  PubMed  Google Scholar 

  33. N. Decaro, G. Elia, V. Martella, M. Campolo, C. Desario, M. Camero, F. Cirone, E. Lorusso, M.S. Lucente, D. Narcisi, P. Scalia, C. Buonavoglia, J. Virol. Methods 133, 92 (2006)

    Article  CAS  PubMed  Google Scholar 

  34. V. Martella, N. Decaro, G. Elia, C. Buonavoglia, J. Vet. Med. B Infect. Dis. Vet. Public Health 52, 312 (2005)

    CAS  PubMed  Google Scholar 

  35. N. Decaro, C. Desario, D.D. Addie, V. Martella, M.J. Vieira, G. Elia, A. Zicola, C. Davis, G. Thompson, E. Thiry, U. Truyen, C. Buonavoglia, Emerg. Infect. Dis. 13, 1222 (2007)

    PubMed  Google Scholar 

  36. N. Decaro, C. Desario, M. Billi, V. Mari, G. Elia, A. Cavalli, V. Martella, C. Buonavoglia, Vet. J. doi:10.1016/j.tvjl.2009.10.027 (2009)

  37. N. Decaro, C. Desario, M. Campolo, G. Elia, V. Martella, D. Ricci, E. Lorusso, C. Buonavoglia, J. Vet. Diagn. Investig. 17, 133 (2005)

    Google Scholar 

  38. H.S. Moon, S.A. Lee, S.G. Lee, R. Choi, S.Y. Jeoung, D. Kim, C. Hyun, Vet. Microbiol. 131, 47 (2008)

    Article  CAS  PubMed  Google Scholar 

  39. N. Decaro, C. Desario, G. Elia, V. Martella, V. Mari, A. Lavazza, M. Nardi, C. Buonavoglia, New Microbiol. 31, 125 (2008)

    PubMed  Google Scholar 

  40. N. Decaro, F. Cirone, C. Desario, G. Elia, E. Lorusso, M.L. Colaianni, V. Martella, C. Buonavoglia, Vet. Rec. 164, 593 (2009)

    CAS  PubMed  Google Scholar 

  41. M.G. Calderon, N. Mattion, D. Bucafusco, F. Fogel, P. Remorini, J. La Torre, J. Virol. Methods 159, 141 (2009)

    Google Scholar 

  42. Z. Demeter, J. Gál, E.A. Palade, M. Rusvai, Vet. Rec. 164, 213 (2009)

    CAS  PubMed  Google Scholar 

  43. K. Hoelzer, L.A. Shackelton, C.R. Parrish, E.C. Holmes, J. Gen. Virol. 89, 2280 (2008)

    Article  CAS  PubMed  Google Scholar 

  44. M. Mochizuki, T. Ohshima, Y. Une, A. Yachi, J. Vet. Med. Sci. 70, 1305 (2008)

    Article  CAS  PubMed  Google Scholar 

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Acknowledgements

The authors would like to thank the help of Borbála Végh in the sample preparation, and the collaboration of all those owners and veterinarians who kindly submitted the analyzed specimens.

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Authors and Affiliations

  1. Department of Pathology and Forensic Veterinary Medicine, Faculty of Veterinary Science, Szent István University, István u. 2, Budapest, 1078, Hungary

    Zoltán Demeter, Elena Alina Palade, Csaba Jakab & Miklós Rusvai

  2. Central Agriculture Office, Directorate of Veterinary Medicinal Products, Szállás u. 8, Budapest, 1107, Hungary

    Tibor Soós & Attila Farsang

Authors
  1. Zoltán Demeter
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  2. Elena Alina Palade
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  3. Tibor Soós
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  5. Csaba Jakab
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  6. Miklós Rusvai
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Correspondence to Zoltán Demeter.

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Demeter, Z., Palade, E.A., Soós, T. et al. Misleading results of the MboII-based identification of type 2a canine parvovirus strains from Hungary reacting as type 2c strains. Virus Genes 41, 37–42 (2010). https://doi.org/10.1007/s11262-010-0478-3

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  • DOI: https://doi.org/10.1007/s11262-010-0478-3

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