Abstract
An outbreak of contagious ecthyma in goats in central Taiwan was investigated. The disease was diagnosed by physical and histopathologic examinations, and the etiology of the disease was identified as orf virus by electron microscopy and polymerase chain reaction (PCR) and sequence of major envelope protein (B2L) gene. The entire protein-coding region of B2L gene were cloned and sequenced. Phylogenetic analysis of B2L amino acid sequences showed that the orf virus identified in this outbreak was closer to the Indian ORFV-Mukteswar 59/05 isolate. This is the first report on the molecular characterization of orf virus in Taiwan.
Similar content being viewed by others
References
F.A. Murphy, E.P.J. Gibbs, M.C. Horzinek, M.J. Studdert, in Veterinary Virology, ed. by F.A. Murphy, E.P.J. Gibbs, M.C. Horzinek, M.J. Studdert (Academic Press, New York, 1999), pp 277–291
E.G. Torfason, S. Gunadottir, J. Clin. Virol. 24, 79 (2002)
A.J. Robinson, A.A. Mercer, Virology 208, 812 (1995)
F. Fenner, J.J. Esposito, in Fields Virology, ed. by D.M. Knipe, P.M. Howley, D.E. Griffin, M.A. Martin, R.A. Lamb, B. Roizman, S.E. Straus (Lippincott, Williams and Wilkins, Philadelphia, PA, 2001), pp 2885–2921
G. Delhon, E.R. Tulman, C.L. Afonso, Z. Lu, A. de la Concha-Bermejillo, H.D. Lehmkuhl, M.E. Piccone, G.F. Kutish, D.L. Rock, J. Virol. 78, 168 (2004)
C. Kottaridi, K. Nomikou, R. Lelli, P. Markoulatos, O. Mangana, J. Virol. Methods 134, 119 (2006)
C. Kottaridi, K. Nomikou, L. Teodori, G. Savini, R. Lelli, P. Markoulatos, O. Mangana, Vet. Microbiol. 116, 310 (2006)
A.A. Mercer, N. Ueda, S.M. Friederichs, K. Hofmann, K.M. Fraser, T. Bateman, S.B. Fleming, Virus Res. 116, 146 (2006)
J.T. Sullivan, A.A. Mercer, S.B. Fleming, A.J. Robinson, Virology 202, 968 (1994)
Y. Inoshima, A. Morooka, H.J. Sentsui, Virol. Methods 84, 201 (2000)
Y. Inoshima, K. Murakami, T. Yokoyama, H. Sentsui, J. Gen. Virol. 82, 1215 (2001)
M.K. Tikkanen, C.J. McInnes, A.A. Mercer, M. Buttner, J. Tuimala, V. Hirvela-Koski, E. Neuvonen, A. Huovilainen, J. Gen. Virol. 85, 1413 (2004)
J. Guo, J. Rasmussen, A. Wunschmann, A. de la Concha-Bermejillo, Vet. Microbiol. 99, 81 (2004)
Y. Kanou, Y. Inoshima, T. Shibahara, Y. Ishidawa, K. Kadota, S. Ohashi, K. Morioka, K. Yoshida, S. Yamad, JARQ 39, 197 (2005)
J. Klein, M. Tryland, Virol. J. 2, 79 (2005)
A. Nitsche, M. Buttner, S. Wilhelm, G. Pauli, H. Meyer, Clin. Chem. 52, 316 (2006)
M. Hosamani, V. Bhanuprakash, A. Scagliarini, R.K. Singh, Vet. Microbiol. 116, 317 (2006)
H.W. Reid, in Disease of Sheep, ed. by W.B. Martin, I.D. Aitken (Oxford, Blackwell scientific publications, London, 1991), pp 265–269
C. Mazur, R.D. Machado, Vet. Rec. 125, 419 (1989)
R.C. Gumbrell, D.A. McGregor, Vet. Rec. 141, 150 (1997)
B. Mondal, A.K. Bera, M. Hosamani, P.A. Tembhurne, S.K. Bandyopadhyay, Vet. Res. Commun. 30, 531 (2006)
J.D. Thompson, D.G. Higgins, T.J. Gibson, Nucleic Acids Res. 22, 4673 (1994)
S. Kumar, K. Tamura, M. Nei, Brief Bioinform. 5, 150 (2004)
J. Guo, Z. Zhang, J.F. Edwards, R.W. Ermel, C. Taylor Jr., A. de la Concha-Bermejillo, Virus Res. 93, 169 (2003)
Acknowledgement
We thank Dr. Shih-Chieh Chang (Department of Veterinary Medicine, National Chung-Hsing University) for helpful discussion. This work was supported in part by a grant from The Bureau of Animal and Plant Health Inspection and Quarantine (BAPHIQ), Council of Agriculture, Taiwan.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Chan, KW., Lin, JW., Lee, SH. et al. Identification and phylogenetic analysis of orf virus from goats in Taiwan . Virus Genes 35, 705–712 (2007). https://doi.org/10.1007/s11262-007-0144-6
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11262-007-0144-6