Skip to main content
SpringerLink
Log in

Construction of a multiprobe for the simultaneous detection of viroids infecting citrus trees

  • Original Article
  • Published:
Virus Genes Aims and scope Submit manuscript

Abstract

Infections with different viroid species are common among cultivated fruit trees and grapevines, and many old-clone citrus varieties contain up to five citrus viroids (CVds) within a single tree. This paper describes the construction of a CVd-Multiprobe consisting of full-length clones of Hop stunt viroid, Citrus exocortis viroid, Citrus bent leaf viroid and CVd-III. The CVd-Multiprobe was tested against RNA transcripts of the four viroids and RNA extracts from plants singly infected with CEVd or HSVd or multiply infected with different CVds. The viroids were effectively diagnosed with the DIG labeled CVd-Multiprobe when tested by Northern hybridization or dot blot analyses. The CVd-Multiprobe does not provide information on the specific viroid resulting in a positive signal. However, this should not be considered as a problem, since most citrus certification programs will discard budwood source trees infected with any of the known CVds.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  1. R.A. Owens, in Encyclopedia of Virology, ed. by R.G. Webster, A Granoff (Academic Press, New York, 1993) pp. 1563–1570

  2. T.O. Diener, Adv. Virus Res. 57, 137–184 (2001)

    Article  PubMed  CAS  Google Scholar 

  3. A. Hadidi, R. Flores, J.W. Randles, J.S. Semancik (eds.), Viroids (CSIRO publishing, 2003), 370 pp

  4. R. Flores, C. Hernandez, A.E. Alba, J.A. Daros, F.D. Serio,Annu. Rev. Phytopathol. 43, 117–139 (2005)

    Article  PubMed  CAS  Google Scholar 

  5. N. Duran-Vila, J.S. Semancik, in Viroids, ed. by A. Hadidi, R. Flores, J.W. Randles, J.S. Semancik (CSIRO Publishing, 2003), pp. 178–194

  6. R. Hadas, M. Bar-Joseph, J.S. Semancik, Ann. Appl. Biol. 115, 515–520 (1989)

    Article  Google Scholar 

  7. M. Bar-Joseph, R. Marcus, R.F. Lee, Ann. Rev. Phytopathol. 27, 291–316 (1989)

    Article  Google Scholar 

  8. C.N. Roistacher, Handbook for Detection and Diagnosis. FAO, Rome. pp. 151–156 (1991)

    Google Scholar 

  9. S.M. Garnsey, J.W. Jones, Plant. Dis. Rep. 51, 410–413 (1967)

    Google Scholar 

  10. M.E. Fonseca, L.H. Marcellino, E.A. Gander, J. Virol. Methods 57, 203–207 (1996)

    Article  PubMed  CAS  Google Scholar 

  11. D. Hanold, J.S. Semancik, R.A. Owens, in Viroids, ed. by A. Hadidi, R. Flores, J.W. Randles, J.S. Semancik (CSIRO Publishing, 2003), pp. 95–102

  12. R.A. Owens, T.O. Diener, Science 213, 670–672 (1981)

    Article  PubMed  Google Scholar 

  13. R.P. Singh, A. Boucher, D.K. Lakshman, S.M. Tavantzis, J. Virol. Methods 49, 221–233 (1994)

    Article  PubMed  Google Scholar 

  14. M. Welnicki, C. Zekanowski, W. Zagorski, Acta. Biochim. Pol. 41, 473–475 (1994)

    PubMed  CAS  Google Scholar 

  15. J. Romero-Durban, M. Cambra, N. Duran-Vila, J. Virol. Methods 55, 37–47 (1995)

    Article  PubMed  CAS  Google Scholar 

  16. P. Stark-Lorenzen, M.C. Guitton, R. Werner, H.P. Muhlbach, Arch. Virol.142, 1289–1296 (1997)

    Article  PubMed  CAS  Google Scholar 

  17. K. Nakahara, T. Hataya, Y. Hayashi, T. Sugimoto, I. Kimura, E. Shikata, J. Virol. Methods 71, 219–227 (1998)

    Article  PubMed  CAS  Google Scholar 

  18. H.P. Muhlbach, U. Weber, G. Gomez, V. Pallas, N. Duran-Vila, A. Hadidi, in Viroids, ed. by A. Hadidi, R. Flores, J.W. Randles, J.S. Semancik (CSIRO Publishing, 2003), pp. 103–114

  19. O. Cohen, O. Batuman, Y. Moskowits, A. Rozov, E. Gootwine, M. Mawassi, M. Bar-Joseph, Phytoparasitica 33, 141–148 (2005)

    Article  Google Scholar 

  20. C.W.Y.F. Wan, R.H. Symons, J. Virol. Methods 63, 57–69 (1997)

    Article  Google Scholar 

  21. A.M. Shamloul, A. Hadidi, J. Virol. Methods 80, 145–155 (1999)

    Article  PubMed  CAS  Google Scholar 

  22. T. Ito, H. Ieki, K. Ozaki, J Virol Methods 106, 235–239 (2002)

    Article  PubMed  CAS  Google Scholar 

  23. A.M. Shamloul, F. Faggioli, J.M. Keith, A. Hadidi, J. Virol. Methods 105, 115–121 (2002)

    Article  PubMed  CAS  Google Scholar 

  24. A.T Hadidi,. Candresse, in Viroids, ed. by A. Hadidi, R. Flores, J.W. Randles, J.S. Semancik (CSIRO Publishing, 2003), pp. 115–122

  25. N. Boonham, L.G. Perez, M.S. Mendez, E.L. Peralta, A. Blockley, K. Walsh, I. Barker, R.A. Mumford, J. Virol. Methods 116, 139–146 (2004)

    Article  PubMed  CAS  Google Scholar 

  26. H. Bostan, X. Nie, and R.P. Singh, J Virol Methods. 116(2), 189–193 (2004)

  27. R.A. Mumford, J. Virol. Methods 116, 139–146 (2004)

    Article  PubMed  CAS  Google Scholar 

  28. E. Ragozzino, F. Faggioli, M. Barba, J. Virol. Methods 121, 25–29 (2004)

    Article  PubMed  CAS  Google Scholar 

  29. A. Ben-Shaul, Y. Guang, N. Mogilner, R. Hadas, M. Mawassi, R. Gafny, M. Bar-Joseph, Phytopathology 85, 359–364 (1995)

    Article  CAS  Google Scholar 

  30. D.J. Lewandowski, W.O. Dawson, Virology 251, 427–437 (1998)

    Article  PubMed  CAS  Google Scholar 

  31. Q. Liu, E.C. Thorland, J.A. Heit, S.S. Sommer, Genome Res.7, 389–398 (1997)

    PubMed  CAS  Google Scholar 

  32. H. Puchta, K. Ramm, R. Luckinger, R. Hadas, M. Bar-Joseph, H.L. Sanger, Nucleic Acids Res. 19, 6640–6645 (1991)

    Article  PubMed  CAS  Google Scholar 

  33. H.J. Gross, H. Domdey, C. Lossow, P. Jank, M. Raba, H. Alberty, H.L. Sanger, Nature 273, 203–208 (1978)

    Article  PubMed  CAS  Google Scholar 

  34. M.C. Herranz, J.A. Sanchez-Navarro, F. Aparicio, V. Pallas, J. Virol. Methods 124, 49–55 (2005)

    Article  PubMed  CAS  Google Scholar 

  35. T. Ito, H. Ieki, K. Ozaki, T. Ito, Arch. Virol. 146, 975–982 (2001)

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

The authors would like to express their gratitude to Dr. A. Hadidi and an anonymous reviewer for his constructive comments and The U.S. Agency for International Development, Bureau for Global Programs, Field Support and Research: Center of Economic Growth and the Israeli Citrus Board for financial support.

Author information

Authors and Affiliations

  1. The S. Tolkowsky laboratory, Department of Virology , Volcani Center, ARO, Bet Dagan, Israel

    Oded Cohen, Ozgur Batuman, Munir Mawassi & Moshe Bar-Joseph

  2. Ajtkhozhin Institute of Molecular Biology and Biochemistry, 480012, Almaty , Kazakstan

    Gulshan Stanbekova

  3. Department of Plant Pathology, Faculty of Agriculture and Life Science, Hirosaki University, Hirosaki , 036-8561, Japan

    Teruo Sano

Authors
  1. Oded Cohen
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ozgur Batuman
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Gulshan Stanbekova
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Teruo Sano
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Munir Mawassi
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Moshe Bar-Joseph
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Moshe Bar-Joseph.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Cohen, O., Batuman, O., Stanbekova, G. et al. Construction of a multiprobe for the simultaneous detection of viroids infecting citrus trees. Virus Genes 33, 287–292 (2006). https://doi.org/10.1007/s11262-006-0067-7

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11262-006-0067-7

Keywords

Search

Navigation