Abstract
The population diversity of Bangladeshi begomoviruses and their vector, Bemisia tabaci was analysed by PCR-based detection and partial genome sequencing. B. tabaci adults and plants expressing symptoms of virus infection were collected from locations representing diverse agro-ecological regions of the country. Universal and species-specific primers were used to detect begomoviruses in seven crops (chilli, okra, papaya, pumpkin, sponge gourd, tomato and yardlong bean) and two common weeds (Ageratum conyzoides and Croton bonplandianum). At least five distinct species of tomato leaf curl viruses infected tomato and other host-plants. Phylogenetic analyses of their nucleotide sequences (∼530 bases) from the intergenic region and capsid protein of DNA-A indicated the existence of five distinct clusters of begomoviruses. Begomoviruses infecting tomato, chilli and dolichos have been reported previously, and those infecting Ageratum, Croton, okra, papaya, pumpkin and yardlong bean are described for the first time. Phylogenetic analyses based on mitochondrial cytochrome oxidase I gene sequences of 21 B. tabaci from Bangladesh and other reference sequences grouped them into at least two independent clusters. Some sequences from different countries, e.g., Bangladesh, China, India, Nepal, Pakistan and Thailand were almost identical while others collected from plants within the same field diverged by as much as 15%, indicating high diversity even at the local level. None of the B. tabaci from Bangladesh grouped with the reference B- and Q-biotype sequences, thus these two aggressive biotypes were apparently absent from Bangladesh.
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References
FAOSTAT data, http://faostat.fao.org/. Accessed September 2005
A.Varma, V.G. Malathi, Ann. Appl. Biol. 142, 145–164 (2003)
S.K. Green, W.S. Tsai, S.L. Shih, L.L. Black, A. Rezajan, M.H. Rashid, M.M.N. Roff, Y.Y. Myint, L.T.A. Hong, Plant Dis. 85, 1286 (2001)
M.N. Maruthi, S.N. Alam, K.A. Kader, A.R. Rekha, A. Cork, J. Colvin, Phytopathology 95, 1472–1481 (2005)
D.R. Jones, Eur. J. Plant Pathol. 109, 195–219 (2003)
L. Hanley-Bowdoin, S.B. Settlage, B.M. Orozoo, S. Nagar, D. Robertson, Crit. Rev. Plant Sci. 18, 71–106 (1999)
N. Navot, E. Pichersky, M. Zeidan, D. Zamir, H. Czosnek, Virology 183, 151–161 (1991)
I.B. Dry, J.E. Rigden, L.R. Krake, P.M. Mullineaux, A. Rezaian, J. Gen. Virol. 74, 147–151 (1993)
K. Saunders, I.D. Bedford, R.W. Briddon, P.G. Markham, S.M. Wong, J. Stanley, Proc. Natl. Acad. Sci. USA 97, 6890–6895 (2000)
R.W. Briddon, S.E. Bull, S. Mansoor, I. Amin, P.G. Markham, Mol. Biotech. 18, 1–4 (2001)
R.W. Briddon, S.E. Bull, I. Amin, S. Mansoor, I.D. Bedford, N. Rishi, S.S. Siwatch, Y. Zafar, A.M. Abdel-Salam, P.G. Markham, Virology 324, 462–474 (2004)
J. Jose, R. Usha, Virology 305, 310–317 (2003)
J.K. Brown, D.R. Frohlich, R.C. Rosell, Ann. Rev. Entomol. 40, 511–534, (1995)
P.J. De Barro, J.W.H. Trueman, D.R. Frohlich, Bull. Entomol. Res. 95, 1–11 (2005)
J.K. Brown, Virus Res. 71, 233–260 (2001)
P.J. De Barro, F. Driver, J.W.H. Trueman, J. Curran, Mol. Phylogenet. Evol. 16, 29–36 (2000)
D.R. Frohlich, I. Torres-Jerez, I.D. Bedford, P.G. Markham, J.K. Brown, Mol. Ecol. 8, 1683–1691 (1999)
J.P. Legg, R. French, D. Rogan, G. Okao-Okuja, J.K. Brown, Mol. Ecol. 11, 1219–1229 (2002)
M.N. Maruthi, J. Colvin, S. Seal, Entomol. Exp. Appl. 99, 13–23 (2001)
M.N. Maruthi, J. Colvin, S. Seal, G. Gibson, J. Cooper, Virus Res. 86, 71–85 (2002)
M.N. Maruthi, J. Colvin, G. Gibson, R.M. Thwaites, G.K. Banks, S.E. Seal, Syst. Entomol. 29, 560–568 (2004)
A.R. Rekha, M.N. Maruthi, V. Muniyappa, J. Colvin, Entomol. Exp. Appl. 117, 221–233 (2005)
P. Sseruwagi, M.N. Maruthi, J. Colvin, M.E.C. Rey, J.K. Brown, J.P. Legg, Entomol. Expt. Appl. 119, 145–153 (2006)
L.P. Zhang, Y.J. Zhang, W.J. Zhang, Q.J. Wu, B.Y. Xu, D. Chu, J. Appl. Entomol. 129, 121–128 (2005)
M.A. Lodhi, G.-N. Ye, N.F. Weeden, B. Reisch, Plant Mol. Biol. Rep. 12, 6–13 (1994)
D. Deng, P.F. McGrath, D.J. Robinson, B.D. Harrison, Ann. Appl. Biol. 125, 327–336 (1994)
M.R. Rojas, R.L. Gilbertson, D.R. Russell, D.P. Maxwell, Plant Dis. 77, 340–347 (1993)
V. Muniyappa, M.N. Maruthi, C.R. Babitha, J. Colvin, R.W. Briddon, K.T. Rangaswamy, Ann. Appl. Biol. 142, 323–331 (2003)
R.V. Chowda Reddy, J. Colvin, V. Muniyappa, S. Seal, Arch. Virol. 150, 845–867 (2005)
D. Swofford, Phylogenetic Analysis Using Parsimony (PAUP) version 4.0 beta., (Sinauer associates, Massachussets, Sunderland 2002)
P.J. De Barro, F. Driver, Aust. J. Entomol. 36, 149–152 (1997)
M.M. Swanson, G.B. Valand, V. Muniyappa, B.D. Harrison, Ann. Appl. Biol. 132, 427–435 (1998)
M. Padidam, R.N. Beachy, C.M. Fauquet, J. Gen. Virol. 76, 249–263 (1995)
J. Jose, R. Usha, Plant Mol. Biol. Rep. 18, 349–355 (2000)
S.E. Bull, W.S. Tsai, R.W. Briddon, P.G. Markham, J. Stanley, S.K. Green, Arch. Virol. 149, 1193–1200 (2004)
S.P. Capoor, P.M. Varma, Curr. Sci. 17, 152–153 (1948)
R.C. Yaraguntaiah, H.C. Govindu, Curr. Sci. 33, 721–722 (1964)
S.K. Raj, N. Aslam, K.M. Srivastava, B.P. Singh, Curr. Sci. 58, 813–814 (1988)
B. Simon, J.L. Cenis, F. Beitia, S. Khalid, I.M. Moreno, A. Fraile, F.G. Arenal, Phytopathology 93, 1422–1429 (2003)
S. Hameed, D.J. Robinson, Arch. Virol. 149, 809–819 (2004)
M.N. Maruthi, J. Colvin, R.W. Briddon, S.E. Bull, V. Muniyappa, J. Plant Pathol. 85, 64–65 (2003)
Acknowledgements
We thank Mr K A Kader for help in the collection of samples. This publication is an output of a project (contract number ICA4-CT-2002–10018) funded by the European Union. The begomoviruses were held under the DEFRA licence PHL 176A/4735.
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Maruthi, M.N., Rekha, A.R., Mirza, S.H. et al. PCR-based detection and partial genome sequencing indicate high genetic diversity in Bangladeshi begomoviruses and their whitefly vector, Bemisia tabaci . Virus Genes 34, 373–385 (2007). https://doi.org/10.1007/s11262-006-0027-2
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DOI: https://doi.org/10.1007/s11262-006-0027-2