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In vitro reactivity and growth inhibition of EPEC serotype O111 and STEC serotypes O111 and O157 by homologous and heterologous chicken egg yolk antibody

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Abstract

IgY is a chicken egg yolk antibody which has been used for treatment and prophylaxis of gastrointestinal infections. Our aim was to verify if IgY obtained from chickens immunized with EPEC O111, STEC O111 and STEC O157 is able to show in vitro reactivity and biological activity towards the three bacteria. IgY was obtained from eggs laid before and after immunization with each bacterium. The preparations of IgY anti-EPEC O111 and anti-STEC O111 shared high reactivity detected by ELISA and growth inhibition ability towards both bacteria EPEC O111 and STEC O111. Nevertheless, the preparation of IgY anti-STEC O157 showed high reactivity and growth inhibitory effect only towards the homologous strain. Our results showing in vitro biological activity of IgY reinforce its use as an alternative for the treatment or prophylaxis of E. coli infections and encourage the development of in vivo studies for a possible future human therapeutic use.

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Abbreviations

AEEC:

attaching and effacing E. coli

BCA:

bicinchoninic acid

BFP:

bundle forming-pillus

BHI:

Brain Heart Infusion

CFA:

Complete Freund’s Adjuvant

cfu:

colony forming unit

E.coli :

Escherichia coli

EPEC:

enteropathogenic E. coli

IgY:

Egg yolk antibodies

LPS:

lipopolysaccharide

OD:

optical density

OPD:

o-phenylenediamine dihydrochloride

P. aeruginosa :

Pseudomonas aeruginosa

SDS-PAGE:

Sodium dodecyl sulphate polyacrylamide gel electrophoresis

S. Enteritidis:

Salmonella Enteritidis

STEC:

Shiga toxin producing E. coli

S. Typhimurium:

Salmonella Typhimurium

TSB:

Tryptic Soy Broth

IgY pre:

IgY from chicken eggs before immunization

IgY post:

IgY from chicken eggs after immunization

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Acknowledgements

This work was supported by Grants 00/02988-3 and 99/05214-0 from the Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP). The authors are most grateful to Rosana Prisco for her help with the statistical analyses, and to Dr. Mário Nakano for his essential support with animal handling and care.

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Authors and Affiliations

  1. Laboratório de Imunogenética, Instituto Butantan, Av. Dr. Vital Brasil, 1500, CEP 05503-900, São Paulo, S.P, Brazil

    José Araujo Amaral, Milene Tino De Franco, Lucy Zapata-Quintanilla & Solange Barros Carbonare

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  1. José Araujo Amaral
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  2. Milene Tino De Franco
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  3. Lucy Zapata-Quintanilla
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  4. Solange Barros Carbonare
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Correspondence to Solange Barros Carbonare.

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Amaral, J.A., De Franco, M.T., Zapata-Quintanilla, L. et al. In vitro reactivity and growth inhibition of EPEC serotype O111 and STEC serotypes O111 and O157 by homologous and heterologous chicken egg yolk antibody. Vet Res Commun 32, 281–290 (2008). https://doi.org/10.1007/s11259-007-9029-3

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  • DOI: https://doi.org/10.1007/s11259-007-9029-3

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