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Deregulated microRNA-22-3p in patients with sepsis-induced acute kidney injury serves as a new biomarker to predict disease occurrence and 28-day survival outcomes

  • Nephrology - Original Paper
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Abstract

Background

Acute kidney injury (AKI) is a common and serious complication of sepsis. MicroRNA-22-3p (miR-22-3p) has been found to be involved in septic AKI progression. The purpose of this study was to analyze both the serum and urinary expression of miR-22-3p in septic AKI patients, and evaluated the clinical value of miR-22-3p in the diagnosis and prognosis of sepsis-induced AKI.

Methods

Serum and urinary expression of miR-22-3p was examined using qRT-PCR. The risk factors related with septic AKI onset were assessed using logistic analysis. A receiver-operating characteristic (ROC) curve was constructed to evaluate the diagnostic performance of miR-22-3p, and the Kaplan–Meier survival curves and Cox regression analysis were used to evaluate the predictive value of miR-22-3p for the 28-day survival of septic AKI patients.

Results

Both serum and urinary miR-22-3p expression was decreased and negatively correlated with kidney injury biomarkers in septic AKI patients. MiR-22-3p expression was a risk factor for AKI onset and had diagnostic accuracy in septic AKI patients. The expression of both serum and urinary miR-22-3p was lower in patients who died, and served as a prognostic biomarker to predict 28-day survival in septic AKI patients.

Conclusion

Serum and urinary miR-22-3p was reduced in sepsis-induced AKI patients, and served as a biomarker to predict AKI occurrence and 28-day survival in sepsis patients.

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Data availability

All data generated or analyzed during this study are included in this published article.

References

  1. Cecconi M, Evans L, Levy M, Rhodes A (2018) Sepsis and septic shock. Lancet 392(10141):75–87

    Article  PubMed  Google Scholar 

  2. Napolitano LM (2018) Sepsis 2018: definitions and guideline changes. Surg Infect (Larchmt) 19(2):117–125

    Article  Google Scholar 

  3. Poston JT, Koyner JL (2019) Sepsis associated acute kidney injury. BMJ 364:k4891

    Article  PubMed  PubMed Central  Google Scholar 

  4. Gomez H, Ince C, De Backer D, Pickkers P, Payen D, Hotchkiss J et al (2014) A unified theory of sepsis-induced acute kidney injury: inflammation, microcirculatory dysfunction, bioenergetics, and the tubular cell adaptation to injury. Shock 41(1):3–11

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  5. Skube SJ, Katz SA, Chipman JG, Tignanelli CJ (2018) Acute kidney injury and sepsis. Surg Infect (Larchmt) 19(2):216–224

    Article  Google Scholar 

  6. Bell M, Granath F, Martensson J, Lofberg E, Ekbom A, Martling CR et al (2009) Cystatin C is correlated with mortality in patients with and without acute kidney injury. Nephrol Dial Transplant 24(10):3096–3102

    Article  CAS  PubMed  Google Scholar 

  7. Uchino S, Bellomo R, Goldsmith D (2012) The meaning of the blood urea nitrogen/creatinine ratio in acute kidney injury. Clin Kidney J 5(2):187–191

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  8. Li X, Yao L, Zeng X, Hu B, Zhang X, Wang J et al (2020) miR-30c-5p alleviated pyroptosis during sepsis-induced acute kidney injury via targeting TXNIP. Inflammation. 44(1):217–228

    Article  Google Scholar 

  9. Ren GL, Zhu J, Li J, Meng XM (2019) Noncoding RNAs in acute kidney injury. J Cell Physiol 234(3):2266–2276

    Article  CAS  PubMed  Google Scholar 

  10. Brandenburger T, Salgado Somoza A, Devaux Y, Lorenzen JM (2018) Noncoding RNAs in acute kidney injury. Kidney Int 94(5):870–881

    Article  CAS  PubMed  Google Scholar 

  11. Zhu J, Lin X, Yan C, Yang S, Zhu Z (2019) microRNA-98 protects sepsis mice from cardiac dysfunction, liver and lung injury by negatively regulating HMGA2 through inhibiting NF-kappaB signaling pathway. Cell Cycle 18(16):1948–1964

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  12. Zheng D, Yu Y, Li M, Wang G, Chen R, Fan GC et al (2016) Inhibition of microRNA 195 prevents apoptosis and multiple-organ injury in mouse models of sepsis. J Infect Dis 213(10):1661–1670

    Article  CAS  PubMed  Google Scholar 

  13. Abou El-Khier NT, Zaki ME, Alkasaby NM (2019) Study of MicroRNA-122 as a diagnostic biomarker of sepsis. Egypt J Immunol 26(2):105–116

    PubMed  Google Scholar 

  14. Guo H, Tang L, Xu J, Lin C, Ling X, Lu C et al (2019) MicroRNA-495 serves as a diagnostic biomarker in patients with sepsis and regulates sepsis-induced inflammation and cardiac dysfunction. Eur J Med Res 24(1):37

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  15. Wu SY, Zhang H, Wu W, Wu YY (2020) Value of serum miR-21-3p in predicting acute kidney injury in children with sepsis. Zhongguo Dang Dai Er Ke Za Zhi 22(3):269–273

    PubMed  Google Scholar 

  16. Zhang J, Wang CJ, Tang XM, Wei YK (2018) Urinary miR-26b as a potential biomarker for patients with sepsis-associated acute kidney injury: a Chinese population-based study. Eur Rev Med Pharmacol Sci 22(14):4604–4610

    CAS  PubMed  Google Scholar 

  17. Ge QM, Huang CM, Zhu XY, Bian F, Pan SM (2017) Differentially expressed miRNAs in sepsis-induced acute kidney injury target oxidative stress and mitochondrial dysfunction pathways. PLoS ONE 12(3):e0173292

    Article  PubMed  PubMed Central  Google Scholar 

  18. Zhang P, Yi L, Qu S, Dai J, Li X, Liu B et al (2020) The biomarker TCONS_00016233 drives septic AKI by targeting the miR-22-3p/AIFM1 signaling axis. Mol Ther Nucleic Acids 19:1027–1042

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  19. Wang X, Wang Y, Kong M, Yang J (2020) MiR-22–3p suppresses sepsis-induced acute kidney injury by targeting PTEN. Biosci Rep. https://doi.org/10.1042/BSR20200527

    Article  PubMed  PubMed Central  Google Scholar 

  20. Levy MM, Fink MP, Marshall JC, Abraham E, Angus D, Cook D et al (2003) 2001 SCCM/ESICM/ACCP/ATS/SIS international sepsis definitions conference. Crit Care Med 31(4):1250–1256

    Article  PubMed  Google Scholar 

  21. Khwaja A (2012) KDIGO clinical practice guidelines for acute kidney injury. Nephron Clin Pract 120(4):c179–c184

    PubMed  Google Scholar 

  22. Mehta RL, Kellum JA, Shah SV, Molitoris BA, Ronco C, Warnock DG et al (2007) Acute Kidney Injury Network: report of an initiative to improve outcomes in acute kidney injury. Crit Care 11(2):R31

    Article  PubMed  PubMed Central  Google Scholar 

  23. Tod P, Roka B, Kaucsar T, Szatmari K, Vizovisek M, Vidmar R et al (2020) Time-dependent miRNA profile during septic acute kidney injury in mice. Int J Mol Sci. 21(15):5316

    Article  CAS  PubMed Central  Google Scholar 

  24. Shen Y, Yu J, Jing Y, Zhang J (2019) MiR-106a aggravates sepsis-induced acute kidney injury by targeting THBS2 in mice model. Acta Cir Bras 34(6):e201900602

    Article  PubMed  PubMed Central  Google Scholar 

  25. Qin Y, Wang G, Peng Z (2019) MicroRNA-191–5p diminished sepsis-induced acute kidney injury through targeting oxidative stress responsive 1 in rat models. Biosci Rep. https://doi.org/10.1042/BSR20190548

    Article  PubMed  PubMed Central  Google Scholar 

  26. Lin Y, Ding Y, Song S, Li M, Wang T, Guo F (2019) Expression patterns and prognostic value of miR-210, miR-494, and miR-205 in middle-aged and old patients with sepsis-induced acute kidney injury. Bosn J Basic Med Sci 19(3):249–256

    CAS  PubMed  PubMed Central  Google Scholar 

  27. Huo R, Dai M, Fan Y, Zhou JZ, Li L, Zu J (2017) Predictive value of miRNA-29a and miRNA-10a-5p for 28-day mortality in patients with sepsis-induced acute kidney injury. Nan Fang Yi Ke Da Xue Xue Bao 37(5):646–651

    CAS  PubMed  Google Scholar 

  28. Liu Y, Guan H, Zhang JL, Zheng Z, Wang HT, Tao K et al (2018) Acute downregulation of miR-199a attenuates sepsis-induced acute lung injury by targeting SIRT1. Am J Physiol Cell Physiol 314(4):C449–C455

    Article  CAS  PubMed  Google Scholar 

  29. Zhu C, Chen T, Liu B (2018) Inhibitory effects of miR-25 targeting HMGB1 on macrophage secretion of inflammatory cytokines in sepsis. Oncol Lett 16(4):5027–5033

    PubMed  PubMed Central  Google Scholar 

  30. Kopp F, Mendell JT (2018) Functional classification and experimental dissection of long noncoding RNAs. Cell 172(3):393–407

    Article  CAS  PubMed  PubMed Central  Google Scholar 

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Authors and Affiliations

  1. Department of Nephrology, The Affiliated Hospital of Qingdao University, No. 16, Jiangsu Road, Qingdao, 266003, Shandong, China

    Hui Zhang, Lin Che, Yanfei Wang, Haiyan Zhou & Xiaofei Man

  2. Department of Respiratory and Critical Care Medicine, The Affiliated Hospital of Qingdao University, No. 16, Jiangsu Road, Qingdao, 266003, Shandong, China

    Haihong Gong & Qian Zhao

Authors
  1. Hui Zhang
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  2. Lin Che
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  3. Yanfei Wang
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  4. Haiyan Zhou
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  5. Haihong Gong
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  6. Xiaofei Man
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  7. Qian Zhao
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Corresponding author

Correspondence to Qian Zhao.

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The authors declare that they have no competing interests.

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A signed written informed consent was obtained from each patient and the experimental procedures were all in accordance with the guideline of the Ethics Committee of The Affiliated Hospital of Qingdao University.

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Written informed consent for publication was obtained from each participant.

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Zhang, H., Che, L., Wang, Y. et al. Deregulated microRNA-22-3p in patients with sepsis-induced acute kidney injury serves as a new biomarker to predict disease occurrence and 28-day survival outcomes. Int Urol Nephrol 53, 2107–2116 (2021). https://doi.org/10.1007/s11255-021-02784-z

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  • DOI: https://doi.org/10.1007/s11255-021-02784-z

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