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A novel rat model of catheter-associated urinary tract infection

  • Urology - Original Paper
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Abstract

Purpose

The authors aimed to establish a rat model of catheter-associated UTIs using a complete urethral catheter. Bacterial growth in biofilms on urethral catheters was analyzed using standard culture methods to validate this model.

Methods

A total of 15 rats were divided into the following three groups according to the duration of indwelling catheter placement: a 2-week group (n = 5, group 1), a 4-week group (n = 5, group 2), and a 6-week group (n = 5, group 3). A urethral catheter was inserted with the distal end buried just beneath the urethra, and it was fixed inside of the urethra with a single suture starting at the vagina so that the suture knot was hidden inside of the vagina, preventing the rats from biting it off. A standard culture method was used to analyze bacterial growth in the biofilms.

Results

All 15 urethral catheters were intact at the end of the experiment. Pseudomonas aeruginosa, Escherichia coli, Enterococcus spp., Enterococcus faecalis, and Corynebacterium spp. were identified in the biofilms on the urethral catheters.

Conclusion

Our rat UTI model consisting of a complete urinary catheter is feasible. Our study may provide fundamental data for future biofilm studies incorporating molecular techniques, and even clinical studies.

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References

  1. Lo E, Nicolle L, Classen D, Arias KM, Podgorny K, Anderson DJ, Burstin H, Calfee DP, Coffin SE, Dubberke ER, Fraser V, Gerding DN, Griffin FA, Gross P, Kaye KS, Klompas M, Marschall J, Mermel LA, Pegues DA, Perl TM, Saint S, Salgado CD, Weinstein RA, Wise R, Yokoe DS (2008) Strategies to prevent catheter-associated urinary tract infections in acute care hospitals. Infect Control Hosp Epidemiol 29(Suppl 1):S41–S50

    Article  PubMed  Google Scholar 

  2. Sedor J, Mulholland SG (1999) Hospital-acquired urinary tract infections associated with the indwelling catheter. Urol Clin North Am 26:821–828

    Article  CAS  PubMed  Google Scholar 

  3. Donlan RM (2002) Biofilms: microbial life on surfaces. Emerg Infect Dis 8:881–890

    Article  PubMed Central  PubMed  Google Scholar 

  4. National Institutes of Health (2002) Research on microbial biofilms (PA-03-047). http://grants.nih.gov/grants/guide/pa-files/PA-03-047.html. Accessed 21 April 2015

  5. Cirioni O, Ghiselli R, Silvestri C, Minardi D, Gabrielli E, Orlando F, Rimini M, Brescini L, Muzzonigro G, Guerrieri M, Giacometti A (2011) Effect of the combination of clarithromycin and amikacin on Pseudomonas aeruginosa biofilm in an animal model of ureteral stent infection. J Antimicrob Chemother 66:1318–1323

    Article  CAS  PubMed  Google Scholar 

  6. Kurosaka Y, Ishida Y, Yamamura E, Takase H, Otani T, Kumon H (2001) A non-surgical rat model of foreign body-associated urinary tract infection with Pseudomonas aeruginosa. Microbiol Immunol 45:9–15

    Article  CAS  PubMed  Google Scholar 

  7. Olson ME, Nickel JC, Khoury AE, Morck DW, Cleeland R, Costerton JW (1989) Amdinocillin treatment of catheter-associated bacteriuria in rabbits. J Infect Dis 159:1065–1072

    Article  CAS  PubMed  Google Scholar 

  8. Hazan Z, Zumeris J, Jacob H, Raskin H, Kratysh G, Vishnia M, Dror N, Barliya T, Mandel M, Lavie G (2006) Effective prevention of microbial biofilm formation on medical devices by low-energy surface acoustic waves. Antimicrob Agents Chemother 50:4144–4152

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  9. Davis CP, Shirtliff ME, Scimeca JM, Hoskins SL, Warren MM (1995) In vivo reduction of bacterial populations in the urinary tract of catheterized sheep by iontophoresis. J Urol 154:1948–1953

    Article  CAS  PubMed  Google Scholar 

  10. Nickel JC, Gristina AG, Costerton JW (1985) Electron microscopic study of an infected foley catheter. Can J Surg 28(50–51):54

    Google Scholar 

  11. Nickel JC, Grant SK, Costerton JW (1985) Catheter-associated bacteriuria. An experimental study. Urology 26:369–375

    Article  CAS  PubMed  Google Scholar 

  12. Lebeaux D, Chauhan A, Rendueles O, Beloin C (2013) From in vitro to in vivo models of bacterial biofilm-related infections. Pathogens 2:288–356

    Article  PubMed Central  PubMed  Google Scholar 

  13. Saint S, Chenoweth CE (2003) Biofilms and catheter-associated urinary tract infections. Infect Dis Clin North Am 17:411–432

    Article  PubMed  Google Scholar 

  14. Pontes DS, Lima-Bittencourt CI, Chartone-Souza E, Amaral Nascimento AM (2007) Molecular approaches: advantages and artifacts in assessing bacterial diversity. J Ind Microbiol Biotechnol 34:463–473

    Article  CAS  PubMed  Google Scholar 

  15. Sontakke S, Cadenas MB, Maggi RG, Diniz PP, Breitschwerdt EB (2009) Use of broad range16S rDNA PCR in clinical microbiology. J Microbiol Methods 76:217–225

    Article  CAS  PubMed  Google Scholar 

  16. Hazan Z, Zumeris J, Jacob H, Raskin H, Kratysh G, Vishnia M, Dror N, Barliya T, Mandel M, Lavie G (2006) Effective prevention of microbial biofilm formation on medical devices by low-energy surface acoustic waves. Antimicrob Agents Chemother 50:4144–4152

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  17. Guiton PS, Hung CS, Hancock LE, Caparon MG, Hultgren SJ (2010) Enterococcal biofilm formation and virulence in an optimized murine model of foreign body-associated urinary tract infections. Infect Immun 78:4166–4175

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  18. Hachem R, Reitzel R, Borne A, Jiang Y, Tinkey P, Uthamanthil R, Chandra J, Ghannoum M, Raad I (2009) Novel antiseptic urinary catheters for prevention of urinary tract infections: correlation of in vivo and in vitro test results. Antimicrob Agents Chemother 53:5145–5149

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  19. Weimer KE, Juneau RA, Murrah KA, Pang B, Armbruster CE, Richardson SH, Swords WE (2011) Divergent mechanisms for passive pneumococcal resistance to beta-lactam antibiotics in the presence of Haemophilus influenzae. J Infect Dis 203:549–555

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  20. Elias S, Banin E (2012) Multi-species biofilms: living with friendly neighbors. FEMS Microbiol Rev 36:990–1004

    Article  CAS  PubMed  Google Scholar 

  21. Madsen JS, Burmølle M, Hansen LH, Sørensen SJ (2012) The interconnection between biofilm formation and horizontal gene transfer. FEMS Immunol Med Microbiol 65:183–195

    Article  CAS  PubMed  Google Scholar 

  22. Tuttle MS, Mostow E, Mukherjee P, Hu FZ, Melton-Kreft R, Ehrlich GD, Dowd SE, Ghannoum MA (2011) Characterization of bacterial communities in venous insufficiency wounds by use of conventional culture and molecular diagnostic methods. J Clin Microbiol 49:3812–3819

    Article  PubMed Central  PubMed  Google Scholar 

  23. Rhoads DD, Wolcott RD, Sun Y, Dowd SE (2012) Comparison of culture and molecular identification of bacteria in chronic wounds. Int J Mol Sci 13:2535–2550

    Article  CAS  PubMed Central  PubMed  Google Scholar 

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Acknowledgments

This study was made possible through the contributions of the research coordinator, Seung June Noh.

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Authors and Affiliations

  1. Department of Urology, St. Vincent’s Hospital, The Catholic University of Korea, College of Medicine, 93 Jungbu-daero, Paldal-gu, Suwon, Gyeonggi-do, 442-723, Republic of Korea

    Hee Youn Kim, Hyun-Sop Choe, Dong Sup Lee, Jae Mo Yoo & Seung-Ju Lee

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  1. Hee Youn Kim
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  2. Hyun-Sop Choe
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  3. Dong Sup Lee
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Correspondence to Seung-Ju Lee.

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Conflict of interest

The authors wish to acknowledge the financial support of the St. Vincent’s Hospital Research Institute of Medical Science.

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Kim, H.Y., Choe, HS., Lee, D.S. et al. A novel rat model of catheter-associated urinary tract infection. Int Urol Nephrol 47, 1259–1263 (2015). https://doi.org/10.1007/s11255-015-1038-5

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  • DOI: https://doi.org/10.1007/s11255-015-1038-5

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