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The role of cell adhesion molecules in ischemic epididymal injury

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Abstract

Objective

The aim of this study was to investigate the role of adhesion molecules in epididymal injury induced by ischemia-reperfusion (I-R) in the rats.

Study design

About 20 male Sprague-Dawley rats were separated into two groups. A sham operation was performed in group 1 (control). In group 2 (I-R), following 6 h of unilateral spermatic cord torsion, 1-h detorsion of the testis was performed. Then, epididymides were removed to measure the tissue levels of malondialdehyde (MDA) and to make histological examination.

Results

Malondialdehyde values increased in group 2. In group 2, the rats demonstrated significant disorganization of the epithelium and loss of microvilli in the epididymal tissue. No abnormal microscopic findings of the epididymis of the rats in the control group. The tenascin expression in the interstitial area of the epididymis was intense in group 2. Intercellular adhesion molecule-1 (ICAM-1) expression by intense brown staining was seen along the basement membrane in epididymal tissue from I-R group rats. The microvillus sites of the epithelia in I-R group were stained mildly by lectin.

Conclusion

The increased expression of adhesion molecules found in epididymal injury induced during of postischemic reperfusion may implicate importance of inflammatory infiltration.

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Authors and Affiliations

  1. Department of Pediatric Surgery, Abanty Izzet Baysal University, Medical School, Bolu, 14280, Turkey

    Hayrettin Ozturk

  2. Department of Pediatric Surgery, Diyarbakir Children Hospital, Diyarbakir, Turkey

    Hulya Ozturk

  3. Department of Pediatric Surgery, Sisli Etfal Training and Research Hospital, Istanbul, Turkey

    Ali Ihsan Dokucu

Authors
  1. Hayrettin Ozturk
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  2. Hulya Ozturk
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  3. Ali Ihsan Dokucu
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Correspondence to Hayrettin Ozturk.

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Ozturk, H., Ozturk, H. & Dokucu, A.I. The role of cell adhesion molecules in ischemic epididymal injury. Int Urol Nephrol 40, 137–142 (2008). https://doi.org/10.1007/s11255-007-9243-5

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  • DOI: https://doi.org/10.1007/s11255-007-9243-5

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