Skip to main content

Advertisement

SpringerLink
Log in

RNA-Seq transcriptome analysis of ileum in Taiping chicken supplemented with the dietary probiotic

  • Regular Articles
  • Published:
Tropical Animal Health and Production Aims and scope Submit manuscript

Abstract

Taiping chicken is indigenous chickens (Gallus gallus domesticus), which was one of China’s excellent poultry species. As the problems caused by the overuse of antibiotics become more and more concern, people begin to look for ways to replace them. Among them, probiotics and their preparations are the research hotspot to replace antibiotics. Probiotics can promote the absorption of nutrients, improve the ratio of meat to meat, resist and prevent diseases, and improve the intestinal tissue morphology. Here, we performed transcriptome profiling of Taiping chicken ileum which was given probiotics by using the Hiseq™ 2500 sequencing platform. A total of 18 genes were differentially expressed in the ileum under control group and probiotics group. Thirteen genes were upregulated with a range of fold change from 1.02 to 8.61, and 5 were downregulated with a range of fold change from − 1.06 to − 2.29. Ten of the differently expressed genes were further validated by qRT-PCR. In addition, Gene ontology and Kyoto Encyclopedia of Genes and Genomes analyses revealed that these differentially expressed genes were mainly enriched to bile secretion, alpha-linolenic acid metabolism, linoleic acid metabolism, ether lipid metabolism, and arachidonic acid metabolism. This study will help us to understand the role of probiotics in indigenous chicken production and provide theoretical basis for the genetic development of indigenous chickens.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7

Similar content being viewed by others

References

  • Aalaei, M., Khatibjoo, A., Zaghari, M., Taherpour, K., Akbari Gharaei, M. and Soltani, M., 2018. Comparison of single- and multi-strain probiotics effects on broiler breeder performance, egg production, egg quality and hatchability. British poultry science, 59, 531–538.

    Article  CAS  PubMed  Google Scholar 

  • Abudabos, A.M., Alyemni, A.H., Yousif, D.M. and Rifat, K.U., 2013. Bacillus subtilis PB6 based probiotic (CloSTAT™) improves intestinal morphological and microbiological status of broiler chickens under Clostridium perfringes challenge. International Journal of Agriculture & Biology, 15, 978–982

    Google Scholar 

  • Aizenstein, M.L., Medeiros, L.F., Medeiros, L.O. and Zanini, A.C., 1977. Inhibition of erythrocyte glycolysis associated with tranylcypromine (maoinhibitor) administration in rabbits: Pyruvate kinase deficiency. General Pharmacology: The Vascular System, 8, 217–219.

    Article  CAS  PubMed  Google Scholar 

  • Ashburner, M., Ball, C.A., Blake, J.A., Botstein, D., Butler, H., Cherry, J.M., Davis, A.P., Dolinski, K., Dwight, S.S., Eppig, J.T., Harris, M.A., Hill, D.P., Issel-Tarver, L., Kasarskis, A., Lewis, S., Matese J.C., Richardson, J.E., Ringwald, M., Rubin, G.M. and Sherlock G., 2000. Gene ontology: tool for the unification of biology. Nature genetics, 25, 25.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Ballatori, N., Christian, W.V., Wheeler, S.G. and Hammond, C.L., 2013. The heteromeric organic solute transporter, OSTα-OSTβ/SLC51: a transporter for steroid-derived molecules. Molecular Aspects of Medicine, 34, 683–692.

    Article  CAS  PubMed  Google Scholar 

  • Boyer, J.L., Trauner, M., Mennone, A., Soroka, C.J., Cai, S.Y., Moustafa, T., Zollner, G., Lee, J.Y. and Ballatori, N., 2006. Upregulation of a basolateral FXR-dependent bile acid efflux transporter OSTalpha–OSTbeta in cholestasis in humans and rodents. American Journal of Physiology Gastrointestinal & Liver Physiology 290, G1124–G1130.

    Article  CAS  Google Scholar 

  • Calkin, A.C. and Tontonoz, P., 2012. Transcriptional integration of metabolism by the nuclear sterol-activated receptors LXR and FXR. Nature Reviews Molecular Cell Biology, 13, 213–224.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Caron, S., Huaman Samanez, C., Dehondt, H., Ploton, M., Briand, O., Lien, F., Dorchies, E., Dumont, J., Postic, C., Cariou, B., Lefebvre, P. and Staels, B., 2013. Farnesoid X Receptor Inhibits the Transcriptional Activity of Carbohydrate Response Element Binding Protein in Human Hepatocytes. Molecular & Cellular Biology, 33, 2202–2211.

    Article  CAS  Google Scholar 

  • Chen, S., Zhou, Y., Chen, Y. and Gu, J., 2018. fastp: an ultra-fast all-in-one FASTQ preprocessor. Bioinformatics, 34, i884–i890

    Article  PubMed  PubMed Central  Google Scholar 

  • Deng, T., Lyon, C.J., Minze, L.J., Lin, J., Zou, J., Liu, J.Z., Ren, Y., Yin, Z., Hamilton, D.J., Reardon, P.R., Sherman, V., Wang, H.Y., Phillips, K.J., Webb, P., Wong, S.T., Wang, R.F. and Hsueh, W.A., 2013. Class II major histocompatibility complex plays an essential role in obesity-induced adipose inflammation. Cell Metabolism, 17, 411–422.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Fang, S., Suh, J.M., Reilly, S.M., Yu, E., Osborn, O., Lackey, D., Yoshihara, E., Perino, A., Jacinto, S., Lukasheva, Y., Atkins, A.R., Khvat, A., Schnabl, B., Yu, R.T., Brenner, D.A., Coulter, S., Liddle, C., Schoonjans, K., Olefsky, J.M., Saltiel, A.R., Downes, M. and Evans, R.M., 2015. Intestinal FXR agonism promotes adipose tissue browning and reduces obesity and insulin resistance. Nature Medicine, 21, 159–165.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Ferrari de Andrade, L., Kumar, S., Luoma, A.M., Ito, Y., Alves da Silva, P.H., Pan, D., Pyrdol, J.W., Yoon, C.H. and Wucherpfennig, K.W., 2020. Inhibition of MICA and MICB Shedding Elicits NK-Cell-Mediated Immunity against Tumors Resistant to Cytotoxic T Cells. Cancer immunology research, 8, 769–780.

    Article  PubMed  Google Scholar 

  • Fujita, N., Oritani, K., Ichii, M., Yokota, T., Saitoh, N., Okuzaki, D., Sekine, Y., Kon, S., Muromoto, R., Saitoh, K., Yoshimura, A., Matsuda, T. and Kanakura, Y., 2014. Signal-transducing adaptor protein-2 regulates macrophage migration into inflammatory sites during dextran sodium sulfate induced colitis. European Journal of Immunology, 44:6, 1791–1801.

  • Gosalvez, M., Perez-Garcia, J. and Weinhouse, S., 1974. Competition for ADP between pyruvate kinase and mitochondrial oxidative phosphorylation as a control mechanism in glycolysis. The FEBS Journal, 46, 133–140.

    CAS  Google Scholar 

  • R.F Gresse, Chaucheyras-Durand, M.A., Fleury, T., Van de Wiele, E., Forano, S., and Blanquet-Diot, S., 2017. Gut microbiota dysbiosis in postweaning piglets: understanding the keys to health, Trends in Microbiology, 25, 851–873.

    Article  CAS  PubMed  Google Scholar 

  • Guan, M., Qu, L., Tan, W., Chen, L. and Wong, C.W., 2011. Hepatocyte nuclear factor-4 alpha regulates liver triglyceride metabolism in part through secreted phospholipase A(2) GXIIB. Hepatology, 53, 458–466

    Article  CAS  PubMed  Google Scholar 

  • Gutierrez-Fuentes, C.G., Zuñiga-Orozco, L.A., Vicente, J.L., Hernandez-Velasco, X., Menconi, A., Kuttappan, V.A., Kallapura, G., Latorre, J., Layton, S., Hargis, B.M. and Téllez, G., 2013. Effect of a lactic acid bacteria based probiotic, Floramax-B11®, on performance, bone qualities, and morphometric analysis of broiler chickens: an economic analysis. Biological Systems, 2, 113.

    Google Scholar 

  • Haas, B.J. and Zody, M.C., 2010. Advancing RNA-Seq analysis. Nature Biotechnology, 28, 421–423.

    Article  CAS  PubMed  Google Scholar 

  • Hattingh, A.L. and Bennie, C.V., 2001. Yogurt as probiotic carrier food. International Dairy Journal, 11, 1–2.

    Article  Google Scholar 

  • Higgins, J.P., Higgins, S.E, Vicente, J.L., Wolfenden, A.D., Tellez, G. and Hargis, B., 2007. Temporal effects of lactic acid bacteria probiotic culture on salmonella in neonatal broilers. Poultry Science, 86:8, 1662–1666.

  • Iqbal, J., Boutjdir, M., Rudel, L.L. and Hussain, M.M., 2014. Intestine-specific MTP and global ACAT2 deficiency lowers acute cholesterol absorption with chylomicrons and HDLs. Journal of Lipid Research, 55, 2261–2275.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Javandel, F., Nosrati, M., van den Hoven, R., Seidavi, A., Laudadio, V. and Tufarelli, V.H., 2019. eracleum persicum. Effects of Hogweed () Powder, Flavophospholipol, and Probiotics as Feed Supplements on the Performance, Carcass and Blood Characteristics, Intestinal Microflora, and Immune Response in Broilers. The Journal of Poultry Science, 56, 262-269.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Jiang, C., Xie, C., Li, F., Zhang, L., Nichols, R.G., Krausz, K.W., Cai, J., Qi, Y., Fang, Z.Z., Takahashi, S., Tanaka, N., Desai, D., Amin, S.G., Albert, I., Patterson, A.D. and Gonzalez, F.J., 2015. Intestinal farnesoid X receptor signaling promotes nonalcoholic fatty liver disease. Journal of Clinical Investigation, 125, 386–402.

    Article  Google Scholar 

  • Johansson, M.E., Sjövall, H. and Hansson, G.C., 2013. The gastrointestinal mucus system in health and disease. Nature Reviews Gastroenterology & Hepatology, 10, 352–361.

    Article  CAS  Google Scholar 

  • Kanehisa, M. and Goto, S., 2000. KEGG: kyoto encyclopedia of genes and genomes. Nucleic acids research, 28, 27–30.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Khobondo, J.O., Muasya, T.K., Miyumo, S., Okeno, T.O., Wasike, C.B., Mwakubambanya, R., Kingori, A.M. and Kahi, A.K., 2015. Genetic and nutrition development of indigenous chicken in Africa. Livestock Research for Rural Development, 27.

  • Kim, D., Langmead, B. and Salzberg, S.L., 2015. HISAT: a fast spliced aligner with low memory requirements. Nature methods, 12, 357.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Kingori, A.M., Wachira, A.M. and Tuitoek, J.K., 2010. Indigenous chicken production in Kenya. International Journal of Poultry Science, 9, 309–316.

    Article  Google Scholar 

  • Koenen, M.E., Kramer, J., van der Hulst, R., Heres, L., Jeurissen, S.H.M. and Boersma, W.J.A., 2004. Immunomodulation by probiotic lactobacilli in layer- and meat-type chickens. British Poultry Science, 45:3, 355–366.

  • Kumar, S. and Barth, A., 2010. Phosphoenolpyruvate and Mg2+ binding to pyruvate kinase monitored by infrared spectroscopy. Biophysical journal, 98, 1931–1940

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Langmead, B. and Salzberg, S.L., 2012. Fast gapped-read alignment with Bowtie 2. Nature methods, 9, 357–359.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Li, J., Li, W., Wang, Z., Khalique, A., Wang, J., Yang, M., Ni, X., Zeng, D., Zhang, D., Zeng, Y., Luo, Q., Jing, B. and Pan, K., 2020a. Screening of immune-related differentially expressed genes from primary lymphatic organs of broilers fed with probiotic bacillus cereus PAS38 based on suppression subtractive hybridization. PLoS ONE, 15, e0235476.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Li, L., Li, W.F., Liu, S.Z. and Wang, H.H., 2020b. Probiotic fermented feed improved the production, health and nutrient utilisation of yellow-feathered broilers reared in high altitude in Tibet. British poultry science, 29. doi: https://doi.org/10.1080/00071668.2020.1801988.

  • Li, Q., Wan, G., Peng, C., Xu, L., Yu, Y., Li, L. and Li, G., 2020c. Effect of probiotic supplementation on growth performance, intestinal morphology, barrier integrity, and inflammatory response in broilers subjected to cyclic heat stress. Animal science journal, 91, e13433.

    Article  CAS  PubMed  Google Scholar 

  • Liu, X., Cao, G., Wang, Q., Yao, X. and Fang, B., 2015. The effect of Bacillus coagulans-fermented and nonfermented Ginkgo biloba on the immunity status of broiler chickens. Journal of Animal Science, 93, 3384–3394

    Article  CAS  PubMed  Google Scholar 

  • Lourenco, M.C., Kuritza, L.N., Westphal, P., Muniz, E., Pickler, L. and Santin, E., 2012. Effects of Bacillus subtilis in the dynamics of infiltration of immunological cells in the intestinal mucosa of chickens challenged with Salmonella Minnesota. International Journal of Poultry Science, 11, 630–634.

    Article  CAS  Google Scholar 

  • Love, M.I., Huber, W. and Anders, S., 2014. Moderated estimation of fold change and dispersion for RNAseq data with DESeq2. Genome biology, 15, 550.

    Article  PubMed  PubMed Central  Google Scholar 

  • Melegy, T., Khaled, N.F., El-Bana, R. and Abdellatif, H., 2011. Effect of dietary supplementation of Bacillus subtilis PB6 (CLOSTAT™) on performance, immunity, gut health and carcass traits in broilers. Journal of American Science, 7, 891–898

    Google Scholar 

  • Mencarelli, A. and Fiorucci, S., 2010. FXR an emerging therapeutic target for the treatment of atherosclerosis. Journal of Cellular and Molecular Medicine, 14, 79–92.

    Article  CAS  PubMed  Google Scholar 

  • Mera, Y., Kawai, T., Ogawa, N., Odani, N., Sasase, T., Miyajima, K., Ohta, T. and Kakutani, M., 2015. JTT-130, a novel intestine-specific inhibitor of microsomal triglyceride transfer protein, ameliorates lipid metabolism and attenuates atherosclerosis in hyperlipidemic animal models. Journal of Pharmacological Sciences, 129, 169–176.

    Article  CAS  PubMed  Google Scholar 

  • Mishima, K., Fujiwara, T., Iki, T., Kuroda, K., Yamashita, K., Tamura, M., Fujisawa, Y. and Watanabe A., 2016. Transcriptome sequencing and profiling of expressed genes in cambial zone and differentiating xylem of Japanese cedar (Cryptomeria japonica), BMC Genomics, 17, 803.

    Article  PubMed  PubMed Central  Google Scholar 

  • Oh, J.K., Pajarillo, E., Chae, J.P., Kim, I.H., Yang, D.S. and Kang, D.K., 2017. Effects of Bacillus subtilis CSL2 on the composition and functional diversity of the faecal microbiota of broiler chickens challenged with Salmonella Gallinarum. Journal of Animal Science and Biotechnology, 8: 1.

    Article  PubMed  PubMed Central  Google Scholar 

  • Omar, R. and Pappolla M., 1993. Oxygen free radicals as inducers of heat shock protein synthesis in cultured human neuroblastoma cells: Relevance to neurodegenerative disease. European Archives of Psychiatry and Clinical Neuroscience. 242, 262–267.

    Article  CAS  PubMed  Google Scholar 

  • Pender, C.M., Kim, S., Potter, T.D., Ritzi, M.M., Young, M. and Dalloul R.A., 2016. Effects of in ovo supplementation of probiotics on performance and immunocompetence of broiler chicks to an Eimeria challenge. Beneficial Microbes, 7, 699–705

    Article  CAS  PubMed  Google Scholar 

  • Pender, C.M., Kim, S., Potter, T.D., Ritzi, M.M., Young, M. and Dalloul, R.A., 2017. In ovo supplementation of probiotics and its effects on performance and immune-related gene expression in broiler chicks. Poultry Science, 96, 1052–1062

    Article  CAS  PubMed  Google Scholar 

  • Pertea, M., Pertea, G.M., Antonescu, C.M., Chang, T.C., Mendell, J.T. and Salzberg, S.L., 2015. String Tie enables improved reconstruction of a transcriptome from RNA-seq reads. Nature biotechnology, 33, 290.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Pertea, M., Kim, D., Pertea, G.M, Leek, J.T. and Salzberg, S.L., 2016. Transcript-level expression analysis of RNA-seq experiments with HISAT, StringTie and Ballgown. Nature protocols, 11, 1650.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Piórkowska, K., Żukowski, K., Połtowicz, K., Nowak, J., Ropka-Molik, K., Derebecka, N., Wesoły, J. and Wojtysiak, D., 2020. Identification of candidate genes and regulatory factors related to growth rate through hypothalamus transcriptome analyses in broiler chickens. BMC Genomics, 21, 509.

    Article  PubMed  PubMed Central  Google Scholar 

  • Prado-Rebolledo, O.F., de Jesus, D M.J., Macedo-Barragan, R.J., Garcia-Márquez, L.J., Morales-Barrera, J.E., Latorre, J.D., Hernandez-Velasco, X. and Tellez, G., 2017. Evaluation of a selected lactic acid bacteria-based probiotic on Salmonella enterica serovar Enteritidis colonisation and intestinal permeability in broiler chickens. Avian Pathology Journal of the W.v.p.a, 46, 90–94.

    Article  PubMed  Google Scholar 

  • Rashidpour, A., Silva-Marrero, J.I., Seguí, L., Baanante, I.V. and Metón, I., 2019. Metformin counteracts glucose-dependent lipogenesis and impairs transdeamination in the liver of gilthead sea bream (Sparus aurata). American Journal of Physiology Regulatory, 316, R265–R273.

    CAS  Google Scholar 

  • Ritossa, F.M., 1962. A New Puffing Pattern Induced by Temperature Shock and DNP in Drosophila. Experientia, 18, 571–573.

    Article  CAS  Google Scholar 

  • Robinson, M.D., McCarthy D.J. and Smyth G.K., 2010. edgeR: a Bioconductor package for differential expression analysis of digital gene expression data. Bioinformatics, 26, 139–140.

    Article  CAS  PubMed  Google Scholar 

  • Saleh, A.A., Paray, B.A. and Dawood, M.A.O., 2020. Olive Cake Meal and Bacillus licheniformis Impacted the Growth Performance, Muscle Fatty Acid Content, and Health Status of Broiler Chickens. Animals (Basel), 10, 695.

    Article  Google Scholar 

  • Sanchez, B., Delgado, S., Blanco-Miguez, A., Lourenco, A., Gueimonde, M. and Margolles, A., 2017. Molecular Nutrition & Food Research, 61(1), http://www.rsc.org/10.1002/mnfr.201600240

  • Sekine, Y., Ikeda, O., Tsuji, S., Yamamoto, C., Muromoto, R., Nanbo, A., Oritani, K., Yoshimura, K. and Matsuda, T., 2009. Signal-transducing adaptor protein-2 regulates stromal cell-derived factor-1 alpha-induced chemotaxis in T cells. Journal of Immunology, 183, 7966–7974.

    Article  CAS  Google Scholar 

  • Skopeliti M, Kratzer U, Altenberend F, Panayotou G, Kalbacher H, Stevanovic S, Voelter W, Tsitsilonis OE., 2007. Proteomic exploitation on prothymosin α- induced mononuclear cell activation. Proteomics, 7, 1814–1824

    Article  CAS  PubMed  Google Scholar 

  • Stęczny, K. and Kokoszyński, D., 2020. Effect of probiotic preparations (EM) on productive characteristics, carcass composition, and microbial contamination in a commercial broiler chicken farm. Animal biotechnology, 17, 1–8.

    Google Scholar 

  • Stephens, H.A., 2001. MICA and MICB genes: can the enigma of their polymorphism be resolved? Trends in immunology, 22, 378–85.

    Article  CAS  PubMed  Google Scholar 

  • Sutcliffe, S., De Marzo, A.M., Sfanos, K.S. and Laurence, M., 2014. MSMB variation and prostate cancer risk: clues towards a possible fungal etiology. Prostate, 74, 569–78.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Tang, J., Shen, X., Ouyang, H., Luo, W., Huang, Y., Tian, Y. and Zhang, X., 2020. Transcriptome analysis of pituitary gland revealed candidate genes and gene networks regulating the growth and development in goose. Animal biotechnology, 11, 1–11.

    Google Scholar 

  • Teo, A.Y. and Tan, H.M., 2007. Evaluation of the performance and intestinal gut microflora of broiler fed on corn-soy diets supplemented with Bacillus subtilis PB6 (CloSTAT™). Journal of Applied Poultry Research, 16, 296–303

    Article  CAS  Google Scholar 

  • Trabelsi, M.S., Daoudi, M., Prawitt, J., Ducastel, S., Touche, V., Sayin, S.I., Perino, A., Brighton, C.A., Sebti, Y., Kluza, J., Briand, O., Dehondt, H., Vallez, E., Dorchies, E., Baud, G., Spinelli, V., Hennuyer, N., Caron, S., Bantubungi, K., Caiazzo, R., Reimann, F., Marchetti, P., Lefebvre, P., Bäckhed, F., Gribble, F.M., Schoonjans, K., Pattou, F., Tailleux, A., Staels, B. and Lestavel, S., 2015. Farnesoid X receptor inhibits glucagon-like peptide-1 production by enteroendocrine L cells. Nature Communications, 6, 7629

    Article  PubMed  Google Scholar 

  • van Wagenberg, C.P.A., van Horne P.L.M. and van Asseldonk M.A.P.M., 2020. Cost-effectiveness analysis of using probiotics, prebiotics, or synbiotics to control Campylobacter in broilers. Poultry science, 99, 4077–4084.

    Article  PubMed  PubMed Central  Google Scholar 

  • Veilleux, A., Mayeur, S., Bérubé, J.C., Beaulieu, J.F., Tremblay, E., Hould, F.S., Bossé, Y., Richard, D. and Levy, E., 2015. Altered intestinal functions and increased local inflammation in insulin-resistant obese subjects: a gene-expression profile analysis. BMC Gastroenterology, 15, 9–16

    Article  Google Scholar 

  • Vineetha, P.G., Tomar, S., Saxena, V.K., Kapgate, M., Suvarna, A. and Adil, K., 2017. Effect of laboratory-isolated Lactobacillus plantarum LGFCP4 from gastrointestinal tract of guinea fowl on growth performance, carcass traits, intestinal histomorphometry and gastrointestinal microflora population in broiler chicken. Journal of Animal Physiology and Animal Nutrition, 101, e362–e370.

    Article  CAS  PubMed  Google Scholar 

  • Wu, X.L., Zhang, Y., Li, Y.T. and Schmidt-Wolf I.G.H., 2020. Increase of Antitumoral Effects of Cytokine-Induced Killer Cells by Antibody-Mediated Inhibition of MICA Shedding. Cancers (Basel), 12, 1818.

    Article  CAS  Google Scholar 

  • Xia, C., He, Z., Cai, Y. and Liang, S.V., 2020. orinostat upregulates MICA via the PI3K/Akt pathway to enhance the ability of natural killer cells to kill tumor cells. European Journal of Pharmacology, 875, 173057.

    Article  CAS  PubMed  Google Scholar 

  • Zhai, M., Guo, Y., Su, A., Tian, H., Sun, G., Kang, X., Li, K. and Yan, F., 2020. Identification of genes related to dexamethasone-induced immunosuppression in chicken thymus using transcriptome analysis. Research in veterinary science, 132, 318–327.

    Article  CAS  PubMed  Google Scholar 

Download references

Funding

This work was supported by the Science and Technology Support Plan Project of Tianshui (No. 2018-FZJHK-7879).

Author information

Authors and Affiliations

  1. College of Biological Engineering and Technology, Tianshui Normal University, South Xihe Road, Qinzhou District, Tianshui, 741000, Gansu Province, People’s Republic of China

    Lintong Luo, Qianning Wang & Fang Ma

Authors
  1. Lintong Luo
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Qianning Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Fang Ma
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Fang Ma.

Ethics declarations

Competing interests

The authors declare that they have no competing interests.

Statement of animal rights

All the methods used in this study were carried out according to the guiding principles of the Chinese Legislation on the Use and Care of Laboratory Animals. Protocol was approved by the Institutional Ethic Committee of Tianshui normal university. (TSNU).

Additional information

Publisher’s note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Supplementary information

ESM 1

(DOCX 15 kb)

ESM 2

(XLSX 4290 kb)

ESM 3

(XLSX 15 kb)

ESM 4

(XLSX 17 kb)

ESM 5

(XLSX 11 kb)

ESM 6

(XLSX 14 kb)

ESM 7

(XLSX 11 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Luo, L., Wang, Q. & Ma, F. RNA-Seq transcriptome analysis of ileum in Taiping chicken supplemented with the dietary probiotic. Trop Anim Health Prod 53, 131 (2021). https://doi.org/10.1007/s11250-021-02566-w

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1007/s11250-021-02566-w

Keywords

Search

Navigation