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High level of B19 strain detection in Brazilian cattle semen

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Abstract

Semen contaminated with microorganisms can disseminate serious diseases including brucellosis. The objectives of this study were to detect Brucella-specific antibodies and Brucella abortus DNA in samples of blood and fresh semen from 100 animals older than 20 months. The samples were collected on farms and in semen collection and processing centers (CCPS). The serum samples were evaluated by Rose Bengal test (RBT). B. abortus DNA was detected by a polymerase chain reaction (PCR) using BAB and IS771 primers. The difference between the vaccine field strain was identified using ery-1, ery-2, and ery-3 primers, using the hemi-nested PCR method. No anti-B. abortus antibodies were detected in the serum samples. Out of the total semen samples, 68% (68/100) presented amplifications of the B. abortus genes. All (68/68) were identified as B19 strain of Brucella abortus vaccine. It was concluded that even bulls that are seronegative for brucellosis can eliminate the bacteria in the semen. The presence in the DNA of the B19 vaccine strain should be investigated for a better understanding of the epidemiological importance of this strain in these animals.

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Funding

This work received financial support from Fundação de Amparo a Pesquisa do Estado de Minas Gerais (FAPEMIG), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq - no. 64/2008).

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Authors and Affiliations

  1. Faculty of Veterinary Medicine, Collaborating Centre of Agricultural Protection in Central Brazil, Federal University of Uberlândia, Ceará St., 2D-33, Uberlândia, Minas Gerais, 38400-902, Brazil

    M. P. S. Lourencetti, M. A. Souza, M. R. Ganda, J. P. Santos, A. Ferreira Júnior & A. M. C. Lima

  2. Biological Institute, 1.252 Conselheiro Rodrigues Alves Av., Vila Mariana, São Paulo, SP, 04014-002, Brazil

    S. Miyashiro

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  1. M. P. S. Lourencetti
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  2. M. A. Souza
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  3. M. R. Ganda
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  4. J. P. Santos
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  5. A. Ferreira Júnior
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  6. S. Miyashiro
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  7. A. M. C. Lima
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Correspondence to J. P. Santos.

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Lourencetti, M.P.S., Souza, M.A., Ganda, M.R. et al. High level of B19 strain detection in Brazilian cattle semen. Trop Anim Health Prod 50, 433–439 (2018). https://doi.org/10.1007/s11250-017-1455-9

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