Abstract
The complement fixation test (CFT), the c-ELISA and an indirect LppQ ELISA were compared to post-mortem (PM) inspection for the diagnosis of contagious bovine pleuropneumonia (CBPP). Sera from 797 cattle in the CBPP affected area of Kazungula, Zambia and 202 sera from Lusaka, Zambia, a CBPP-free area were used. The clinical history of CBPP was recorded and all the cattle from Kazungula were slaughtered and PM inspections conducted. The prevalence of CBPP in Kazungula was 67.5% (95%CI 67.2%, 70.8%), 52.6% (95%CI 49.2%, 56.2%), 59.0% (95%CI 55.5%, 62.4%) and 44.4% (95%CI 41.0%, 47.9%) using PM inspection, CFT, c-ELISA and LppQ ELISA, respectively. Three of the 202 negative control animals tested positive on the c-ELISA although they were from a known CBPP negative zone. In this study, the c-ELISA was more sensitive in detecting cattle with lesions in the chronic stage than any other test whilst the CFT detected more during the onset stage. No single serological test could detect all stages of CBPP infection, therefore the use of more than one test is advised.
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Abbreviations
- CBPP:
-
Contagious bovine pleuropneumonia
- c-ELISA:
-
Competitive enzyme-linked immunosorbent assay
- CFT:
-
Complement fixation test
- IAEA:
-
International atomic energy agency
- PM:
-
Post-mortem
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Acknowledgements
We are greatly indebted to the International Atomic Energy agency (IAEA) who funded this study. We thank, in particular, Dr. Hermann Unger from IAEA for his contribution to the work. We also acknowledge the cooperation received from the farmers during their most difficult time. We are further grateful to the staff under the Ministry of Livestock and Fisheries both at the Central Veterinary Research Institute (CVRI) and the field for the help during the study.
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Muuka, G., Hang’ombe, B.M., Nalubamba, K.S. et al. Comparison of complement fixation test, competitive ELISA and LppQ ELISA with post-mortem findings in the diagnosis of contagious bovine pleuropneumonia (CBPP). Trop Anim Health Prod 43, 1057–1062 (2011). https://doi.org/10.1007/s11250-011-9805-5
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DOI: https://doi.org/10.1007/s11250-011-9805-5