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Prevalence of porcine reproductive and respiratory syndrome virus (PRRSV) antigen-positive uterine tissues in gilts culled due to reproductive disturbance in Thailand

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Abstract

The objective of the present study was to determine the prevalence of porcine reproductive and respiratory syndrome virus (PRRSV) antigen-positive uterine tissue in gilts culled due to reproductive disturbance in relation to age at culling, reasons for culling, herds, and PRRSV vaccination. Uterine tissues of 100 gilts from six swine herds in Thailand were collected. The immunohistochemistry was performed to detect the PRRSV antigen using a polymer-based non-avidin–biotin technique. PRRSV was detected in the cytoplasm of the macrophages in the subepithelial connective tissue layers of the endometrium in 33.0% of the culled gilts. The detection of PRRSV antigen varied among the herds from 14.3% to 80.0% (P = 0.018). The detection of PRRSV in the uterine tissues at different ages was not statistically different (29.6%, 39.4%, and 40.9% in gilts culled at 6–8, 9–10, and 11–16 months of age, respectively, P = 0.698), similar to the reasons for culling (P = 0.929). PRRSV antigen was found in 24.5% of the gilts vaccinated against the EU-strain-modified-live PRRSV vaccine and in 23.1% of the gilts vaccinated against the US-strain-modified-live PRRSV (P = 0.941). The level of antibody titers against PRRSV had no impact on PRRSV antigen detection in the uterine tissues. Similarly, the detection of PRRSV antigen did not differ between the virgin gilts (35.4%) and the gilts mated before culling (30.8%) (P = 0.622). It can be concluded that PRRSV remains in the uterine tissue of the infected gilts for several months even though vaccinations and acclimatization have been carried out.

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Acknowledgements

Financial support for the present study was provided by the National Research Council of Thailand. E. Olanratmanee is a grantee of the Royal Golden Jubilee (RGJ) Ph.D. Program, the Thailand Research Fund. Language revision of the manuscript is coordinated by Chula Unisearch, Chulalongkorn University.

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Authors and Affiliations

  1. Department of Obstetrics, Gynecology, and Reproduction, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand, 10330

    Em-on Olanratmanee, Annop Kunavongkrit & Padet Tummaruk

  2. Department of Pathology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand, 10330

    Supradit Wangnaitham & Roongroje Thanawongnuwech

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  1. Em-on Olanratmanee
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  2. Supradit Wangnaitham
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  3. Roongroje Thanawongnuwech
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  4. Annop Kunavongkrit
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Correspondence to Padet Tummaruk.

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Olanratmanee, Eo., Wangnaitham, S., Thanawongnuwech, R. et al. Prevalence of porcine reproductive and respiratory syndrome virus (PRRSV) antigen-positive uterine tissues in gilts culled due to reproductive disturbance in Thailand. Trop Anim Health Prod 43, 451–457 (2011). https://doi.org/10.1007/s11250-010-9713-0

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