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Evaluation of mammary gland immunity and therapeutic potential of Tinospora cordifolia against bovine subclinical mastitis

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Abstract

Enhancement of the diseased mammary gland immunity and therapeutic potential of hydro-methanolic extract of Tinospora cordifolia (T. cordifolia; stem) in bovine subclinical mastitis was investigated. Somatic cell count (SCC), total bacterial count (TBC), phagocytic activity, and leukocyte lysosomal enzymes like myeloperoxidase and acid phosphatase activity and Interleukin-8 (IL-8) level were evaluated after intramammary infusion of hydro-methanolic extract (stem) of T.cordifolia in diseased cows. The qualitative analysis of the extract revealed the presence of polysaccharide, phenol, alkaloid, and protein. Intramammary infusion of hydro-methanolic extract of T. cordifolia treatment initially enhanced the SCC; thereafter, significant reduction in cell count (P < 0.05) was observed on day 15 of the treatment period, however, reduction in TBC was observed from day 3 onwards. The phagocytic activity of milk polymorphonuclear cells enhanced in the diseased cows treated with the T. cordifolia extract. Similarly, the lysosomal enzyme content of the milk polymorphonuclear cells enhanced significantly (P < 0.05) in diseased cows treated with T. cordifolia. The IL-8 level in milk serum also increased significantly (P < 0.05) in diseased cows treated with the herb extract. The results suggest that the hydro-methanolic extract of T.cordifolia (stem) possesses antibacterial and immunomodulatory properties. In the present study, the biological activity of the Tinospora cordifolia extract at standardized dose against bovine subclinical mastitis is reported for the first time. Development of alternative therapy with medicinal plants is an option for livestock farmers who are not allowed to use the conventional allopathic drugs under certain farming system or cannot afford to use allopathic drugs.

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Abbreviations

AP:

Acid phosphatase

IL-8:

Interleukin-8

MPO:

Myloperoxidase

PMNs:

Polymorphonuclear cells

SCC:

Somatic cell count

SCM:

Sub clinical mastitis

TBC:

Total bacterial count

T. cordifolia :

Tinospora cordifolia

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Acknowledgements

The authors thank the Director, IVRI, Head, Division of Medicine, for providing necessary facilities.

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Authors and Affiliations

  1. I/C, Preventive Medicine Laboratory, Division of Medicine, Indian Veterinary Research Institute, Izatnagar, 243122, U.P., India

    Reena Mukherjee & G. C. Ram

  2. NAARM, Hyderabad, A.P., India

    U. K. De

Authors
  1. Reena Mukherjee
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  2. U. K. De
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  3. G. C. Ram
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Correspondence to Reena Mukherjee.

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Mukherjee, R., De, U.K. & Ram, G.C. Evaluation of mammary gland immunity and therapeutic potential of Tinospora cordifolia against bovine subclinical mastitis. Trop Anim Health Prod 42, 645–651 (2010). https://doi.org/10.1007/s11250-009-9471-z

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  • DOI: https://doi.org/10.1007/s11250-009-9471-z

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