Skip to main content
SpringerLink
Log in

Sero-prevalence and identification of Ornithobacterium rhinotracheale in broiler flocks in south-eastern Iran

  • Published:
Tropical Animal Health and Production Aims and scope Submit manuscript

Abstract

Ornithobacterium rhinotracheale is a gram negative bacterial pathogen causing respiratory tract infections in poultry. Tracheal, lung and serum samples were obtained from 21 broiler flocks of 8 farms from a slaughterhouse located in south-eastern of Iran. Among 630 tracheal and lung samples from samples resulting from 315 chickens, 11 (3.5%) ORT isolates were identified using biochemical tests. The isolates originated from 9 (42.9%) flocks out of 4 farms. All of the isolates were recovered from tracheal swabs and showed an API 20NE identification biocode 0-2-2-0-0-0-4. Of the 420 serum samples examined by ELISA, 134 (31.9%) sera from 17 (81.0%) flocks were positive for ORT antibodies. These results indicate that ORT is present in most broiler flocks with respiratory disorders in southeast Iran.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

Abbreviations

ADH:

arginine dihydrolase

DIA:

dot immunobinding assay

ELISA:

enzyme linked immunosorbent assay

OD:

optical density

ORT:

Ornithobacterium rhinotracheale

References

  • Allymehr, M., 2006. Seroprevalence of Ornithobacterium rhinotracheale infection in broiler and broiler breeder chickens in west Azerbaijan province, Iran. Journal of Veterinary Medicine series A, 53, 40–42

    Article  CAS  PubMed  Google Scholar 

  • Canal, C.W., Leao, J.A., Ferreira, D.J., Macagnan, M., Pipi Salle, C.T. and Back, A., 2003. Prevalence of antibodies against Ornithobacterium rhinotracheale in broilers and breeders in Southern Brazil. Avian Disease, 47, 731–737 doi:10.1637/6090

    Article  Google Scholar 

  • Canal, C.W., Leao, J.A., Rocha, S.L.S., Macagnan, M., Lima-Rosaa, C.A.V., Oliveiraa, S.D. and Back, A., 2005. Isolation and characterization of Ornithobacterium rhinotracheale from chickens in Brazil. Research in Veterinary Science, 78, 225–230 doi:10.1016/j.rvsc.2004.10.003

    Article  CAS  PubMed  Google Scholar 

  • Chansiripornchai, N., Wanasawaeng, W. and Sasipreeyajan, J., 2007. Seroprevalence and identification of Ornithobacterium rhinotracheale from broiler and broiler breeder flocks in Thailand. Avian Disease, 51, 777–780 doi:10.1637/0005-2086(2007)51[777:SAIOOR]2.0.CO;2

    Article  Google Scholar 

  • Charlton, B.R., Channing-Santiago, S.E., Bickford, A.A., Cardona, C.J., Chin, R.P., Coopeer G.L, Droual, R., Jeffrey, J.S., Meteyer, C.U., Shivaprasad, H.L. and Walker, R.L., 1993. Preliminary characterization of a pleomorphic gram-negative rod associated with avian respiratory disease. Journal of Veterinary Diagnostic Investigation, 5, 47–51

    CAS  PubMed  Google Scholar 

  • Chin, R.P., van Empel, P.C.M. and Hafez, H.M., 2008. Ornithobacterium rhinotracheale Infection. In: Saif, Y.M. et al. Diseases of Poultry. 12th Edition. Iowa State University Press. Ames, Iowa, USA. pp., 765–774

    Google Scholar 

  • Erganis, O., Hadimli, H.H., Kav, K., Çorlu, M. and Ozturk, D., 2002. A comparative study on detection of Ornithobacterium rhinotracheale antibodies in meat type turkeys by dot immuno binding assay, rapid agglutination test and serum agglutination test. Avian Pathology, 31, 201–204 doi:10.1080/03079450120118685

    Article  CAS  PubMed  Google Scholar 

  • Hafez, H.M., 1998. Current status on the Laboratory diagnosis of Ornithobacterium rhinotracheale “ORT” in poultry. Berliner und Münchener Tierärztliche Wochenschrift; 111, 143–145

    CAS  PubMed  Google Scholar 

  • Hafez, H.M., 2002. Diagnosis of Ornithobacterium rhinotracheale. International Journal of Poultry Science, 1, 114–118 doi:10.3923/ijps.2002.114.118

    Article  Google Scholar 

  • Hafez, H.M., Kruse, W., Emele, J. and Sting, R., 1993. Eine Atemwegsinfektion bei Mastputen durch Pasteurella ähnliche Erreger: Klinik, Diagnostik und Therapie. Proceedings of International Conference on Poultry Diseases, Potsdam, Germany, pp. 105–112

    Google Scholar 

  • Lopes, V., Rajashekara, G., Back, A., Shaw, D.P., Halvorson, D.A. and Nagaraja, K.V., 2000. Outer membrane proteins for serologic detection of Ornithobacterium rhinotracheale infection in turkeys. Avian Diseases, 44, 957–962 doi:10.2307/1593072

    Article  CAS  PubMed  Google Scholar 

  • Murthy, T.R.G.K., Dorairajan, N., Balasubramanium, G.A., Dinakaran, A.M. and Saravanabava, K., 2008. In vitro antibiotic sensitivity of Ornithobacterium rhinotracheale strains isolated from laying hens in India. Veterinarski Arhiv, 78, 49–56

    CAS  Google Scholar 

  • Odor, E.M., Salem, M., Pope, C.R., Sample, B., Primm, M., Vance, K. and Murphy, M., 1997. Isolation and identification of Ornithobacterium rhinotracheale from commercial broiler flocks on the Delmarva Peninsula. Avian Disease, 41, 257–260 doi:10.2307/1592468

    Article  CAS  Google Scholar 

  • Ozbey, G., Ongor, H., Balik, D.T., Celik, V., Kilic, A. and Muz, A., 2004. Investigations on Ornithobacterium rhinotracheale in broiler flocks in Elazig province located in the East of Turkey. Veterinary Medicine- Czech, 49, 305–311

    Google Scholar 

  • Ozbey, G., Ertas, H.B. and Muz, A., 2005. Random amplified polymorphic DNA (RAPD) analysis of Ornithobacterium rhinotracheale strains isolated from chickens in Turkey. Veterinary Medicine- Czech, 50, 526–530

    CAS  Google Scholar 

  • Pattison, M., McMullin, P.F., Bradbury, J.M. and Alexander D.J., 2008. Poultry Disease. 6th edn. Saunders Elsevier, London, pp. 164–170

    Google Scholar 

  • Rahimi, M. and Banani, M., 2007. Isolation of Ornithobacterium rhinotracheale from the chickens of a broiler farm in Kermanshah province, west of Iran. Iranian Journal of Veterinary Research, 8, 355–359

    Google Scholar 

  • Refai, M., El-Gohary, A., Attia, S.A. and Khalifa, R.A., 2005. Diagnosis of Ornithobacterium rhinotracheale infection in chickens by ELISA. Egyptian Journal of Immunology, 12, 87–93

    CAS  PubMed  Google Scholar 

  • Saif, Y.M., Fadly, A.M., Glisson, J.R., McDougald, L.R., Nolan, L.K. and Swayne, D.E., 2008. Diseases of poultry. 12th edn. Blackwell Publishing, London, pp. 765–771

    Google Scholar 

  • Schuijffel, D.F., vanEmpel, P.C.M., Pennings, A.M.M.A., vanPutten, J.P.M. and Nuijten, P.J.M., 2005. Successful selection of cross-protective vaccine candidates for Ornithobacterium rhinotracheale infection. Infection and Immunity, 73, 6812–6821 doi:10.1128/IAI.73.10.6812-6821.2005

    Article  CAS  PubMed  Google Scholar 

  • Szalay, D., Glávits, R., Nemes, C., Kósa, A. and Fodor, L., 2002. Clinical signs and mortality caused by Ornithobacterium rhinotracheale in turkey flocks. ACTA Veterinaria Hungarica, 50, 297–305 doi:10.1556/AVet.50.2002.2.6

    Article  CAS  PubMed  Google Scholar 

  • Tabatabai, L.B., Zehr, E.S., Zimmerli, M.K. and Nagaraja, K.V., 2008. Iron acquisition by Ornithobacterium rhinotracheale. Avian Disease, 52, 419–425 doi:10.1637/8185-113007-Reg

    Article  Google Scholar 

  • Thachil, A.J., Velayudhan, B.T., Lopes-Berkas, V.C., Halvorson, D.A. and Nagaraja, K.V., 2007. Application of polymerase chain reaction finger printing to differentiate Ornithobacterium rhinotracheale isolates. Journal of Veterinary Diagnostic Investigation, 19, 417–420

    PubMed  Google Scholar 

  • Tsai, H.J. and Huang, C.W., 2006. Phenotypic and molecular characterization of isolates of Ornithobacterium rhinotracheale from chickens and pigeons in Taiwan. Avian Disease, 50, 502–507 doi:10.1637/7527-031906R.1

    Article  Google Scholar 

  • Turan, N. and Ak, S., 2002. Investigation of the presence of Ornithobacterium rhinotracheale in chickens in Turkey and determination of the seroprevalance of the infection using the enzyme-linked immunosorbent assay. Avian Disease, 46, 442–446 doi:10.1637/0005-2086(2002)046[0442:IOTPOO]2.0.CO;2

    Article  CAS  Google Scholar 

  • Turkyilmaz, S., 2005. Isolation and serotyping of Ornithobacterium rhinotracheale from poultry. Turkish Journal of Veterinary and Animal Sciences. 29, 1299–1304

    Google Scholar 

  • Turkyilmaz, S. and Kaya, O., 2005. Detection of antibodies produced against Ornithobacterium rhinotracheale and Bordetella avium by enzyme-linked immunosorbent assay in hens and turkeys in Aydin province, Turkey. Turkish Journal of Veterinary and Animal Sciences. 29, 897–902

    CAS  Google Scholar 

  • van Empel, P.C.M. and Hafez, H.M., (1999): Ornithobacterium rhinotracheale: A review. Avian Pathology, 28, 217–227 doi:10.1080/03079459994704

    Article  Google Scholar 

  • van Empel, P.C.M., van den Bosch, H., Loeffen, P. and Storm, P., 1997. Identification and serotyping of Ornithobacterium rhinotracheale. Journal of Clinical Microbiology, 35, 418–421

    PubMed  Google Scholar 

  • Vega, V., Zepeda, A., Ramírez, S., Morales, V., Fernández, P., de Oca, R.M., Guerra-Infante, F.M., de Jesús de Haro-Cruz, M., Blackall, P.J. and Soriano, E.V., 2008. Hemagglutinating activity of serovar reference strains of Ornithobacterium rhinotracheale. Journal of Veterinary Diagnostic Investigation, 20, 353–355

    PubMed  Google Scholar 

Download references

Acknowledgements

The Research Council of Shahid Bahonar University of Kerman is gratefully acknowledged for financial support of this work. The authors are thankful to the managers of the Eslam Kish industrial poultry slaughterhouse of Kerman for their cooperation.

Author information

Authors and Affiliations

  1. Department of Microbiology, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, P.O. Box 7616914111, Kerman, Iran

    Reza Ghanbarpour

  2. Department of Poultry Diseases, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran

    Mahmood Salehi

Authors
  1. Reza Ghanbarpour
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Mahmood Salehi
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Reza Ghanbarpour.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Ghanbarpour, R., Salehi, M. Sero-prevalence and identification of Ornithobacterium rhinotracheale in broiler flocks in south-eastern Iran. Trop Anim Health Prod 41, 1679–1683 (2009). https://doi.org/10.1007/s11250-009-9365-0

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11250-009-9365-0

Keywords

Search

Navigation