Abstract
This study aimed to determine the effect of intranasal exposure to low doses of Pasteurella multocida B:2 on survival of goats challenged with high doses of the same organism. Eighteen goats were selected and divided into three groups. Goats of group 1 were exposed intranasally twice, with a two-week interval, to 7 × 106 cfu/ml of live P. multocida B:2. Goats of group 2 were not exposed to P. multocida B:2 but were kept together with the exposed group 1. Goats of group 3 remained as unexposed controls and were kept separated from the other two groups. Serum samples were collected at weekly intervals to determine the antibody levels. At week 5 post exposure, all goats were challenged subcutaneously with 3.7 × 1010 cfu/ml of live P. multocida B:2. Following challenge exposure, 8 (67%) goats (4 goats from each of groups 1 and 2) were killed owing to haemorrhagic septicaemia. Four goats were killed peracutely within 48 h post challenge, while the other four goats were killed acutely between 2 and 4 days post challenge. None of the goats of group 3 were killed for haemorrhagic septicaemia. Goats of groups 1 and 2 showed significantly (p < 0.05) higher antibody levels following the first intranasal exposure to P. multocida B:2. However, only group 1 retained the significantly (p < 0.05) high antibody levels following a second intranasal exposure, and remained significantly (p<0.05) higher than groups 2 and 3 at the time of challenge. P. multocida B:2 was successfully isolated from various organs of goats that were killed between 1 and 4 days post challenge.
Article PDF
Similar content being viewed by others
Abbreviations
- HS:
-
haemorrhagic septicaemia
- cfu:
-
colony-forming unit
- PCR:
-
polymerase chain reaction
References
Bain, R.V.S., De Alwis, M.C.L., Carter, G.R. and Gupta, B.K., 1982. Haemorrhagic septicaemia. FAO Animal Health and Production paper No. 33, (Food and Agricultural Organization of the United Nations, Rome)
Biswal, A., Shivachandra, S.B., Saxena, M.K., Kumar, A.A., Singh, V.P. and Srivastava, S.K., 2004. Molecular variability among strains of Pasteurella multocida isolated among outbreaks of haemorrhagic septicaemia in India. Veterinary Research Communication, 28, 287–298
Carter, G.R. and De Alwis, M.C.L., 1989. Haemorrhagic septicaemia. In: C. Adlam and J.M. Rutter (eds), Pasteurella and Pasteurellosis, (Academic Press, London), 131–160
Chandrasekaran, S., Kennett, L., Yeap, P.C., Muniandy, N., Rani, B. and Mukkar, T.K.S., 1994. Characterisation of immune response and duration of protection in buffaloes immunised with haemorrhagic septicaemia vaccine. Veterinary Microbiology, 41, 213–219
De Alwis, M.C.L., Wijewardana, T.G., Gomis, A.I.U. and Vipulasiri, A.A., 1990. Persistence of the carrier status in haemorrhagic septicaemia (Pasteurella multocida serotype 6:B infection) in buffaloes. Tropical Animal Health and Production, 22, 185–194
Francis, M.J., 1993. Subunit vaccines and vector. In: R.A. Peters (ed), Vaccines for Veterinary Applications, (Butterworth-Heinemann, Oxford), 31–32
Hirabayashi, Y., Kurata, H., Funato, H., Nagamine, T., Aizawa, C., Tamura, S., Shimada, K. and Kurata, T., 1990. Comparison of intranasal inoculation of influenza HA vaccine combined with cholera toxin B subunit with oral or parenteral vaccination. Vaccine, 8, 243–248
Horadagoda, N.U., Hodgson, J.C., Moon, G.M., Wijewardana, T.G. and Eckersall, P.D., 2002. Development of a clinical syndrome resembling haemorrhagic septicaemia in the buffalo following intravenous inoculation of Pasteurella multocida serotype B:2 endotoxin and the role of tumour necrosis factor-alpha. Research in Veterinary Science, 72, 194–200
McGhee, J.R. and Kiyono, H., 1993. New prespectives in vaccine development: mucosal immunity to influenza. Infectious Agents and Diseases, 2, 55–73
Myint, A., Jones, T.O. and Nyunt, H.H., 2005. Safety, efficacy and cross-protectivity of a live intranasal aerosol haemorrhagic septicaemia vaccine. Veterinary Record, 156, 41–45
Ogra, P.L., Faden, H. and Welliver, R.C., 2001. Vaccination strategies for mucosal immunity responses. Clinical Microbiology Reviews, 14, 430–445
Saharee, A.A., Salim, N.B., Rasedee, A. and Jainudeen, M.R., 1993. Haemorrhagic septicaemia carriers among cattle and buffalo in Malaysia. Pasteurellosis in Production Animals, (ACIAR Proceedings no. 43), 89–91
Wijewardana, T.G., De Alwis, M.C.L. and Vipulasiri, A.A., 1986. An investigation into the possible role of the goat as a host in haemorrhagic septicaemia. Sri Lanka Veterinary Journal, 34, 24–32
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Zamri-Saad, M., Ernie, Z.A. & Sabri, M.Y. Protective effect following intranasal exposure of goats to live Pasteurella multocida B:2. Trop Anim Health Prod 38, 541–546 (2006). https://doi.org/10.1007/s11250-006-4437-x
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11250-006-4437-x