Abstract
Occasionally, chimeras do not transmit the gene of interest to pups in gene disruption experiments. However, the risk of failure could be reduced if we could identify embryonic stem (ES)-derived germ cells in the testis. Here, we report the production of pups from three lines of infertile chimeric male mice and the establishment of knockout lines by combining green fluorescent protein-tagged ES cells with intracytoplasmic sperm injection.
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Acknowledgments
We thank Y. Esaki, K. Kawata and Y. Koreeda for technical support. This work was partly supported by the Ministry of Education, Culture, Sports, Science and Technology of Japan.
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Fujihara, Y., Kaseda, K., Inoue, N. et al. Production of mouse pups from germline transmission-failed knockout chimeras. Transgenic Res 22, 195–200 (2013). https://doi.org/10.1007/s11248-012-9635-x
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DOI: https://doi.org/10.1007/s11248-012-9635-x