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DNA-binding, antioxidant activity and in vitro cytotoxicity induced by ruthenium(II) complexes containing polypyridyl ligands

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Abstract

Two new ruthenium(II) polypyridyl complexes, [Ru(dmp)2(maip)](ClO4)2 1 (maip = 2-(3-aminophenyl)imizado[4,5-f][1,10]phenanthroline and [Ru(dmp)2(paip)](ClO4)2 2 (paip = 2-(4-aminophenyl)imidazo[4,5-f][1,10]phenanthroline, dmp = 2, 9-dimethyl-1,10-phenanthroline) have been synthesized and characterized. The DNA-binding behaviors of complexes 1 and 2 were studied by viscosity measurements, thermal denaturation, and absorption titration. The results show that the two complexes intercalate between the base pairs of DNA. The DNA-binding constants K b for complexes 1 and 2 were determined to be 3.23 ± 0.16 × 104 M−1 (s = 0.97) and 4.34 ± 0.65 × 104 M−1 (s = 1.13). The cytotoxicity of these complexes has been evaluated by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. The IC50 values are 35.70, 41.04, 55.25 and 37.50 for complex 1 and 37.02, 103.08, 130.07 and 37.80 for complex 2 against BEL-7402, C-6, HepG-2 and MCF-7 cell lines, respectively. The antioxidant activity against hydroxyl radical (OH•) was also investigated.

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Acknowledgments

This work is supported by the National Nature Science Foundation of China (30800227), the Science and Technology Foundation of Guangdong Province (2009B030803057) and Guangdong Pharmaceutical University for financial support.

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Authors and Affiliations

  1. School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou, 510006, People’s Republic of China

    Cheng-Hui Zeng, Yun-Jun Liu, Zheng-Zheng Li & Zhen-Hua Liang

  2. School of Life Science and Biopharmaceutical, Guangdong Pharmaceutical University, Guangzhou, 510006, People’s Republic of China

    Hong-Liang Huang

  3. School of Public Health, Guangdong Pharmaceutical University, Guangzhou, 510006, People’s Republic of China

    Fu-Hai Wu

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  1. Cheng-Hui Zeng
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  2. Yun-Jun Liu
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Correspondence to Fu-Hai Wu.

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Zeng, CH., Liu, YJ., Li, ZZ. et al. DNA-binding, antioxidant activity and in vitro cytotoxicity induced by ruthenium(II) complexes containing polypyridyl ligands. Transition Met Chem 35, 731–736 (2010). https://doi.org/10.1007/s11243-010-9386-6

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