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Microspore derived population developed from an inter-specific hybrid (Brassica oleracea × B. carinata) through a modified protocol provides insight into B genome derived black rot resistance and inter-genomic interaction

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Abstract

Inter-specific hybridization between different species of Brassicas is widely practiced to broaden the genetic base and inter-genomic transfer of desirable traits. A large population of inter-specific hybrids (B. oleracea × B. carinata) were developed through direct embryo culture for introgression of black rot resistance from B into C sub-genome of Brassicas. Inter-specific hybrids were resistant to race 1 and 4 of black rot. A large set of microspores derived (MD) plants were developed using partially fertile inter-specific hybrids for the first time using a modified culture density, temperature shock treatment and polyamine supplement. Forty-three B and 323 C sub-genome specific SSRs were analysed in 117 MD plants along with their ploidy analysis. Frequent occurrence of unreduced and B. carinata like gametes and large number of anueploids in the MD plants was because of single set of B sub-genome (BCC) in the mother plant. Aneuploids for C sub-genome indicate meiotic abnormality in paring between C sub-genome of B. oleracea and B. carinata. Role of B7 and B5 for resistance to Xcc1 and B6 and B2 for resistance to Xcc4 was established. The unique MD population developed will be instrumental in broadening the genetic base of B. oleracea and studying the genetics for various traits using chromosome addition/deletion lines and aneuploids.

Key Message

Microspore derived plants from a sesquidiploid (Brassica oleracea × B. carinata) elucidates B genome conferred black rot resistance (Xcc1 and Xcc4) and meiotic abnormalities.

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Acknowledgements

We are thankful to SERB, Department of Science and Technology (Govt. of India) for providing research grant (Grant number: SR/FT/LS-73/2011- S. S. Dey and SB/FT/LS-236/2012- Reeta Bhatia). We are also thankful to ICAR-Indian Agricultural Research Institute, New Delhi for providing support in conducting the research work.

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Authors and Affiliations

  1. Division of Floriculture and Landscaping, ICAR-Indian Agricultural Research Institute, New Delhi, 110012, India

    Reeta Bhatia

  2. ICAR-Indian Agricultural Research Institute, Regional Station, Katrain, Kullu, HP, 175129, India

    Kanika Sharma, Chander Parkash & Achintya Pramanik

  3. Division of Plant Pathology, ICAR-Indian Agricultural Research Institute, New Delhi, 110012, India

    Dinesh Singh

  4. Division of Vegetable Science, ICAR-Indian Agricultural Research Institute, New Delhi, 110012, India

    Saurabh Singh, Raj Kumar & S. S. Dey

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  1. Reeta Bhatia
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  2. Kanika Sharma
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  3. Chander Parkash
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  4. Achintya Pramanik
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  5. Dinesh Singh
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Contributions

SSD and RB conceptualised the research work and involved in data analysis and preparing the manuscript. KS, SS, CP, AP and RK are involved in conducting the laboratory and field experiment, data recording and preparation of manuscript. DS and AP has done the disease screening of the plants for Xcc.

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Correspondence to S. S. Dey.

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Communicated by A. M. R. Ferrie.

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Bhatia, R., Sharma, K., Parkash, C. et al. Microspore derived population developed from an inter-specific hybrid (Brassica oleracea × B. carinata) through a modified protocol provides insight into B genome derived black rot resistance and inter-genomic interaction. Plant Cell Tiss Organ Cult 145, 417–434 (2021). https://doi.org/10.1007/s11240-021-02018-1

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