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Infection with Micromonospora strain SB3 promotes in vitro growth of Lolium multiflorum plantlets

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Abstract

Cattle breeding is an important economical activity in Argentina, highly dependent on grass production. In the last decades, grasslands zones were reduced and confined to less productive lands due to the advance of agronomical cultures. Therefore, it is important to develop new strategies to improve forage production. New eco-friendly trends in plant growth promotion include the use of microbial endophytes, but the in vitro studies of plant-bioinoculant interactions is limited by the scarce current technological development. In this work, we use a micropropagation protocol for Lolium multiflorum, developed in a previous work, to study the effect of bacterization with actinobacterial endophytes, isolated from Argentine native grasses, on the growth of L. multiflorum in vitro plantlets. To achieve this objective, L. multiflorum plantlets were inoculated with three Micromonospora strains (SB3, TW2.1 and TW2.2). The results obtained showed that the effect of actinobacterial inoculation depends on the Micromonospora strain used. The inoculation with SB3 promoted plant growth, increasing plant biomass, root length and the rate of plantlets ready to be acclimatized after 4 weeks of in vitro culture. Strain TW2.1 did not show, statistically, differences compared to control treatments, while TW2.2 inhibited plant growth, decreasing plant biomass, root length and the rate of plants ready to acclimatize. Our results showed that Micromonospora strain SB3 could be a good candidate to use in breeding programs for L. multiflorum and other grasses to increase their yield.

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Acknowledgements

This work was supported by funding from Agencia Nacional de Promoción Científica y Tecnológica (PICT 2014-3315) CONICET (Consejo Nacional de Investigaciones Científicas y Técnicas, Argentina) Grant PIP 11220150100956CO and from Universidad de Buenos Aires UBACyT 20020150100067BA and 20020150200075BA. Doctors Regalado Gonzalez and Della Mónica are Post-Doctoral Fellows at CONICET.

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Authors and Affiliations

  1. Facultad de Ciencias Exactas y Naturales, Departamento de Biodiversidad y Biología Experimental, Laboratorio de Micología y Fitopatología No. 69, Universidad de Buenos Aires, Ciudad Autónoma de Buenos Aires, Argentina

    I. F. Della Mónica, M. V. Novas & L. J. Iannone

  2. CONICET - Universidad de Buenos Aires, Instituto de Micología y Botánica (INMIBO), Ciudad Autónoma de Buenos Aires, Argentina

    I. F. Della Mónica, M. V. Novas, L. J. Iannone, S. I. Pitta-Alvarez & J. J. Regalado

  3. Universidad Nacional de General Sarmiento, Instituto de Ciencias, Los Polvorines, Buenos Aires, Argentina

    G. Querejeta

  4. CONICET - Universidad Nacional de Comahue, Instituto de Investigaciones en Biodiversidad y Medio Ambiente (INIBIOMA), Bariloche, Argentina

    J. M. Scervino

  5. Facultad de Ciencias Exactas y Naturales, Departamento de Biodiversidad y Biología Experimental, Laboratorio de Cultivo Experimental de Plantas y Microalgas No. 68, Universidad de Buenos Aires, Ciudad Autónoma de Buenos Aires, Argentina

    S. I. Pitta-Alvarez & J. J. Regalado

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  1. I. F. Della Mónica
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  2. M. V. Novas
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  3. L. J. Iannone
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  4. G. Querejeta
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  5. J. M. Scervino
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  6. S. I. Pitta-Alvarez
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Contributions

DMIF designed and executed the in vitro experiments, isolated and identified the actinobacterial strains used and collaborated in the writing of the manuscript. NMV performed the statistical analysis, provided the plant material and collaborated in the improvement of the manuscript. ILJ realized the strains molecular pairwise comparison, provided the plant material and critically review the manuscript and improve the text. GQ did the IAA quantification, analyzing the statistic differences among strains and collaborate in the manuscript improvement. SJM did the molecular identification of the actinobacteria and critically review the manuscript. PASI designed the in vitro experiments, revised the manuscript critically for important intellectual content and reviewed the English of the manuscript. RJJ designed and executed the in vitro experiments and wrote the manuscript.

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Correspondence to J. J. Regalado.

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Communicated by Sergio J. Ochatt.

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Della Mónica, I.F., Novas, M.V., Iannone, L.J. et al. Infection with Micromonospora strain SB3 promotes in vitro growth of Lolium multiflorum plantlets. Plant Cell Tiss Organ Cult 134, 445–455 (2018). https://doi.org/10.1007/s11240-018-1434-5

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