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A high production of flavonoids and anthraquinones via adventitious root culture of Oplopanax elatus and evaluating antioxidant activity

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Abstract

Adventitious root (AR) culturing is an effective approach for obtaining bioactive compounds from the endangered plant species of Oplopanax elatus Nakai. In the present study, flavonoids and anthraquinones, both important bioactive compounds were identified via HPLC-ESI–MS/MS, and flavonoid monomers of quercetin and kaempferide and anthraquinone monomers of aloe-emodin, rhein, and emodin were detected in O. elatus AR cultures. To enhance synthesis of both compounds, methyl jasmonate (MeJA) was used as the elicitor to treat ARs. At 200 µM, MeJA significantly increased the contents of quercetin, aloe-emodin, rhein, and emodin, whereas 225 µM was the optimal concentration for kaempferide accumulation. The antioxidant property in ARs was evaluated by determining activities of 1,1-diphenyl-2-picrylhydrazyl (DPPH) and alkyl radical scavenging using the an electron spin resonance spectrometer. The IC50 of DPPH (6.2 µg/mL) and alkyl (20.2 µg/mL) radical scavenging activities with MeJA treatment were lower than that of the control, thereby indicating that ARs treated with MeJA possess stronger antioxidant activity. Hence, AR cultures can be deployed as raw materials or additives in the production of antioxidant-related O. elatus products using the MeJA as elicitor.

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Acknowledgements

This study was sponsored by the Jilin Scientific and Technological Development Program (20180101278JC) and the National Natural Science Foundation of China (31660080 and 21662038).

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Authors and Affiliations

  1. Key Laboratory of Natural Resources of Changbai Mountain and Functional Moleculars (Yanbian University), Ministry of Education, Yanji, 133002, Jilin, China

    L. Han, X. C. Piao, J. Jiang, X. L. Jiang, C. R. Yin & M. L. Lian

Authors
  1. L. Han
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  2. X. C. Piao
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  3. J. Jiang
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  4. X. L. Jiang
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  5. C. R. Yin
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  6. M. L. Lian
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Contributions

LH and XLJ performed AR culture experiments. XCP and JJ conducted the experiment of antioxidant experiment. CRY analyzed bioactive compounds. MLL designed experiments and wrote the manuscript.

Corresponding author

Correspondence to M. L. Lian.

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The authors declare that they have no conflict of interest.

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Communicated by Konstantin V. Kiselev.

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Key Message

Methyl jasmonate enhanced accumulation of flavonoid and anthraquinone monomers in adventitious roots of Oplopanax elatus.

Extract from adventitious roots elicited by methyl jasmonate exerted a high antioxidant activity.

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Han, L., Piao, X.C., Jiang, J. et al. A high production of flavonoids and anthraquinones via adventitious root culture of Oplopanax elatus and evaluating antioxidant activity. Plant Cell Tiss Organ Cult 137, 173–179 (2019). https://doi.org/10.1007/s11240-018-01543-w

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  • DOI: https://doi.org/10.1007/s11240-018-01543-w

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