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BpMADS12 mediates endogenous hormone signaling: effect on plant development Betula platyphylla

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Abstract

BpMADS12 is one of the SOC1-like genes isolated from Betula platyphylla. The expression pattern of BpMADS12 was detected in both vegetative and reproductive organs by real-time quantitative RT-PCR and promoter analyses. Analysis of the promoter region suggested that BpMADS12 is involved in abiotic stress and hormone responses. In this study, BpMADS12 was expressed in transgenic birch under the control of the CaMV 35S promoter. Our results showed that the transgene had no effect on flowering time, whereas it affected the number and size of female inflorescences and decreased the seed vigor. Plant morphology was also affected; the height and ground diameter were significantly greater in the transgenic birch than in nontransgenic line (NT). Levels of brassinosteroid (BR) and zeatinriboside (ZR) were higher in transgenic lines than in NT due to the overexpression of BpMADS12 in birch, which resulted in the up-regulation of some BR and ZR biosynthesis and signaling genes. We propose that BpMADS12is involved in hormone biosynthesis and signal-mediated growth and development.

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Acknowledgments

This work was financially supported by The Fundamental Research Funds for the Central Universities (No. DL12CA04) and the National Science and Technology Program of China during the 12th Five-Year Plan Period (No. 2013AA102704).

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Authors and Affiliations

  1. State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, 26 Hexing Road, Harbin, 150040, China

    Huiyu Li, Danyang Wu, Zijia Wang, Feifei Liu, Guifeng Liu & Jing Jiang

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  1. Huiyu Li
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  2. Danyang Wu
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Correspondence to Jing Jiang.

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Li, H., Wu, D., Wang, Z. et al. BpMADS12 mediates endogenous hormone signaling: effect on plant development Betula platyphylla . Plant Cell Tiss Organ Cult 124, 169–180 (2016). https://doi.org/10.1007/s11240-015-0885-1

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