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Enhancement strategies of bioactive compound production in adventitious root cultures of Eleutherococcus koreanum Nakai subjected to methyl jasmonate and salicylic acid elicitation through airlift bioreactors

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Abstract

To establish an attractive method for the annual production of valuable Eleutherococcus koreanum-based bioactive compounds, the adventitious roots in airlift bioreactors were subjected to methyl jasmonate (MJ) and salicylic acid (SA) treatment for 1 week before harvest as a chemical elicitor to enhance the productivity of bioactive compounds. After 6 weeks of culture, the addition of 50 µmol MJ was found to be the optimal concentration and elicitor type for biomass and bioactive compound production in the roots, as evidenced by the highest biomass and total production of five target bioactive compounds (i.e., eleutherosides B and E, chlorogenic acid, and total phenolics and flavonoids) without causing any physiological disorders. The total production of the target bioactive compounds at 50 µmol MJ (303.93 mg L−1) was 37.77 % higher than that for the control treatment, and the addition of high concentrations of MJ and SA increased 2,2-diphenyl-1-picrylhydrazyl activity and hydrogen peroxide content in the roots. In addition, the adventitious roots subjected to 50 µmol MJ for 1 week had a competitive edge for commercial use, as evidenced by the high total production of the five target bioactive compounds (per 1 L of medium) when compared to the 3-year field-grown plants.

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Abbreviations

DPPH:

2,2-Diphenyl-1-picrylhydrazyl

DW:

Dry weight

FW:

Fresh weight

H2O2 :

Hydrogen peroxide

HPLC:

High-performance liquid chromatography

IBA:

Indole-3-butyric acid

MS:

Murashige and Skoog

MJ:

Methyl jasmonate

ROS:

Reactive oxygen species

SA:

Salicylic acid

TDZ:

Thidiazuron; N-phenyl-N′-1,2,3,-thidiazol-5-ylurea

vvm:

Air volume per culture volume per min

References

  • Ahmed S, Nawata E, Hosokawa M, Domae Y, Sakuratani T (2002) Alterations in photosynthesis and some antioxidant enzymatic activities of mungbean subjected to waterlogging. Plant Sci 163:117–123

    Article  CAS  Google Scholar 

  • Ahn JK, Park SY, Lee WY, Lee JJ (2005) Effects of growth regulators on adventitious root growth and eleutherosides and chlorogenic acid accumulation in air lift bioreactor cultures of Eleutherococcus koreanum. Kor J Plant Biotechnol 32:57–61

    Article  Google Scholar 

  • Ali MB, Yu KY, Hahn EJ, Paek KY (2005) Differential responses of anti-oxidants enzymes, lipoxygenase activity, ascorbate content and the production of saponins in tissue cultured root of mountain Panax ginseng C.A. Meyer and Panax quinquefolius L. in bioreactor subjected to methyl jasmonate stress. Plant Sci 169:83–92

    Article  CAS  Google Scholar 

  • Ali MB, Yu KY, Hahn EJ, Paek KY (2006) Methyl jasmonate and salicylic acid elicitation induces ginsenosides accumulation, enzymatic and non-enzymatic antioxidant in suspension culture Panax ginseng roots in bioreactors. Plant Cell Rep 25:613–620

    Article  CAS  PubMed  Google Scholar 

  • Baque MA, Moh SH, Lee EJ, Zhong ZZ, Paek KY (2012) Production of biomass and useful compounds from adventitious roots of high-value added medicinal plants using bioreactor. Biotechnol Adv 30:1255–1267

    Article  CAS  PubMed  Google Scholar 

  • Belhadj A, Teleg N, Saigne C, Cluzet S, Barrieu F, Hamdi S, Merillon JM (2008) Effect of methyl jasmonate in combination with carbohydrates on gene expression of PR proteins, stilbene and anthocyanin accumulation in grapevine cell cultures. Plant Physiol Biochem 46:493–499

    Article  CAS  PubMed  Google Scholar 

  • Choi SM, Son SH, Yun SE, Kwon OW, Seon JH, Paek KY (2000) Pilot-scale culture of adventitious roots of ginseng in a bioreactor system. Plant Cell Tiss Organ Cult 62:187–193

    Article  CAS  Google Scholar 

  • Chong TM, Abdullah MA, Fadzillah NM, Lai OM, Lajis NH (2005) Jasmonic acid elicitation of anthraquinones with some associated enzymic and non-enzymic antioxidant responses in Morinda elliptica. Enz Microb Technol 36:469–477

    Article  CAS  Google Scholar 

  • Cui L, Wang ZY, Zhou XH (2012) Optimization of elicitors and precursors to enhance valtrate production in adventitious roots of Valeriana amurensis Smir. ex Kom Plant Cell Tiss Organ Cult 108:411–420

  • Dong X (1998) SA, JA, ethylene, and disease resistance in plants. Curr Opin Plant Biol 1:316–323

    Article  CAS  PubMed  Google Scholar 

  • Frankfater CR, Dowd MK, Triplett BA (2009) Effect of elicitors on the production of gossypol and methylated gossypol in cotton hairy roots. Plant Cell Tiss Organ Cult 98:341–349

    Article  CAS  Google Scholar 

  • Fujikawa T, Yamaguchi A, Morita I, Takeda H, Nishibe S (1996) Protective effects of Acanthopanax senticosus Harms form Hokkaido and its components on gastric ulcer in restrained cold water stressed rats. Biol Pharm Bull 19:1227–1230

    Article  CAS  PubMed  Google Scholar 

  • Houot V, Etienne P, Petitot AS, Barbier S, Blein JP, Suty L (2001) Hydrogen peroxide induces programmed cell death features in cultured tobacco BY-2 cells, in a dose-dependent manner. J Exp Bot 52:1721–1730

    Article  CAS  PubMed  Google Scholar 

  • Hu FX, Zhong JJ (2008) Jasmonic acid mediates gene transcription of ginsenoside biosynthesis in cell cultures of Panax notoginseng treated with chemically synthesized 2-hydroxyethyl jasmonate. Proc Biochem 43:113–118

    Article  CAS  Google Scholar 

  • Hung KT, Hsu YT, Kao CH (2006) Hydrogen peroxide is involved in methyl jasmonate-induced senescence of rice leaves. Physiol Plant 127:293–303

    Article  CAS  Google Scholar 

  • Jwa CS, Yang YT, Koh JS (2000) Changes in free sugars, organic acids, free amino acids and minerals by harvest time and parts of Acanthopanax koreanum. J Kor Soc Agric Chem Biotechnol 43:106–109

    CAS  Google Scholar 

  • Kang SM, Jung HY, Kang YM, Yun DJ, Bahk JD, Yang JK, Choi MS (2004) Effects of methyl jasmonate and salicylic acid on the production of tropane alkaloids and the expression of PMT and H6H in adventitious root cultures of Scopolia parviflora. Plant Sci 166:745–751

    Article  CAS  Google Scholar 

  • Kang JY, Jeong YH, Lim EH, Lee EJ, Chu HM, Jeong SJ (2005) Analysis of acanthoic acid and isomers in Acanthopanax species by gas chromatography. J Pharm Sci 20:41–46

    Google Scholar 

  • Kim HK, Bang CS, Choi YM, Lee JS (2007) Antioxidant and antiproliferative activities of methanol extracts from leafy vegetables consumed in Korea. Food Sci Biotechnol 16:802–806

    CAS  Google Scholar 

  • Ko HJ, Song CK, Cho NK (2003) Growth of seedling and germination characteristics of Acanthopanax koreanum Nakai. Kor J Med Crop Sci 11:46–52

    Google Scholar 

  • Lee EJ, Paek KY (2012) Effect of nitrogen source on biomass and bioactive compound production of in submerged cultures of Eleutherococcus koreanum Nakai adventitious roots. Biotechnol Prog 28:508–514

    Article  CAS  PubMed  Google Scholar 

  • Lee EJ, Kim MK, Paek KY (2010) Auxin and cytokinin affect biomass and bioactive compound production from adventitious roots of Eleutherococcus koreanum. Kor J Hort Sci Technol 28:678–684

    Google Scholar 

  • Li SW, Xue L, Xu S, Feng H, An L (2009) Hydrogen peroxide acts as signal molecule in the adventitious roots formation of mungbean seedlings. Env Exp Bot 65:63–71

    Article  CAS  Google Scholar 

  • Ma B, Zhang Q, Liu YH, Li J, Xu QY, Li XT, Yang XJ, Yao D, Sun JJ, Cui GB, Ying HJ (2013) Simultaneous determination of Eleutheroside B and Eleutheroside E in rat plasma by high performance liquid chromatography-electrospray ionization mass spectrometry and its application in a pharmacokinetic study. J Chromatogr B 917–918:84–92

    Article  Google Scholar 

  • Meena K, Cheruvathur T, Thomas D (2014) Effect of plant growth regulators and elicitors on rhinacanthin accumulation in hairy root culture of Rhinacanthus nasutus (L.) Kurz. Plant Cell Tiss Organ Cult 118:169–177

    Article  Google Scholar 

  • Melillo MT, Leonettil P, Bongiovanni M, Astagnone SP, Bleve ZT (2006) Modulation of reactive oxygen species actives and H2O2 during compatible and incompatible tomato-root-knot nematode interactions. New Phytol 170:501–512

    Article  CAS  PubMed  Google Scholar 

  • Murashige T, Skoog F (1962) A revise medium for rapid growth and bioassay with tobacco tissue culture. Physiol Plant 15:473–497

    Article  CAS  Google Scholar 

  • Murthy HM, Hahn EJ, Paek KY (2008) Adventitious roots and secondary metabolism. Chin J Biotechnol 24:711–716

    Article  CAS  Google Scholar 

  • Murthy HM, Lee EJ, Paek KY (2014) Production of secondary metabolites from cell and organ cultures: strategies and approaches for biomass improvement and metabolite accumulation. Plant Cell Tiss Organ Cult 118:1–16

    Article  CAS  Google Scholar 

  • Shohael AM, Murthy HN, Lee HL, Hahn EJ, Islam R, Paek KY (2008) Increased eleutheroside production in Eleutherococcus senticosus embryogenic suspension cultures with methyl jasmonate treatment. Biochem Eng J 38:270–273

    Article  CAS  Google Scholar 

  • Sivakumar G, Medina-Bolivar F, Lay JO, Dolan MC, Condori J, Grubbs SK, Wright SM, Baque MA, Lee EJ, Paek KY (2011) Bioprocess and bioreactor: next generation technology for production of potential plant-based antidiabetic and antioxidant molecules. Curr Med Chem 18:79–90

    Article  CAS  PubMed  Google Scholar 

  • Sivanandhan G, Dev GK, Jeyaraj M, Rajesh M, Arjunan A, Muthuselvam M, Manickavasagam M, Selvaraj N, Ganapathi A (2013) Increased production of withanolide A, withanone, and withaferin A in hairy root cultures of Withania somnifera (L.) Dunal elicited with methyl jasmonate and salicylic acid. Plant Cell Tiss Organ Cult 114:121–129

    Article  CAS  Google Scholar 

  • Slacanin I, Marston A, Hostettmann K (1991) The isolation of Eleutherococcus senticosus constituents by centrifugal partition chromatography and their quantitative determination by high performance liquid chromatography. Phytochem Anal 2:137–142

    Article  CAS  Google Scholar 

  • Theboral J, Sivanandhan G, Subramanyam K, Arun M, Selvaraj N, Manickavasagam M, Ganapathi A (2014) Enhanced production of isflavones by elicitation in hairy root culture of soybean. Plant Cell Tiss Organ Cult 117:477–481

    Article  CAS  Google Scholar 

  • Wang J, Man S, Gao W, Zhang L, Huang L (2013) Cluster analysis of ginseng tissue cultures, dynamic change of growth, total saponins, and specific oxygen uptake rate in bioreactor and immuno-regulative effect of ginseng adventitious root. Ind Crops Prod 41:57–63

    Article  Google Scholar 

  • Yu KW, Gao W, Hahn EJ, Paek KY (2002) Jasmonic acid improves ginsenoside accumulation in adventitious root culture of Panax ginseng C.A. Meyer. Biochem Eng J 11:211–215

    Article  CAS  Google Scholar 

  • Zhu S, Bai YJ, Oya MK, Tanaka K, Komatsu KSK, Maruyama TKR, Goda YHR, Kawasaki TKS, Masao Fujita MSO, Shibata TSR (2011) Genetic and chemical diversity of Eleutherococcus senticosus and molecular identification of Siberian ginseng by PCR-RFLP analysis based on chloroplast trnK intron sequence. Food Chem 129:1844–1850

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This work was conducted during the research year of Chungbuk National University in 2013.

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Authors and Affiliations

  1. Well Green Co. Ltd., Chungbuk National University, Cheongju, 361-763, Republic of Korea

    Eun Jung Lee

  2. Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University, Cheongju, 361-763, Republic of Korea

    So Young Park & Kee Yoeup Paek

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  1. Eun Jung Lee
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Correspondence to Kee Yoeup Paek.

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Lee, E.J., Park, S.Y. & Paek, K.Y. Enhancement strategies of bioactive compound production in adventitious root cultures of Eleutherococcus koreanum Nakai subjected to methyl jasmonate and salicylic acid elicitation through airlift bioreactors. Plant Cell Tiss Organ Cult 120, 1–10 (2015). https://doi.org/10.1007/s11240-014-0567-4

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