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A new and efficient micropropagation method and its breeding applications in Asparagus genera

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Abstract

Cultivated asparagus (Asparagus officinalis L.) is an economically important plant worldwide. “Morado de Huetor” is a Spanish autochthonous landrace characterized by their longevity, organoleptic characteristics, differential biocompound content and high heterozygosity, resulting in heterogeneous plantations with limited productivity. Consequently, this landrace suffers high risk of extinction due the lack of productivity. The preservation of the genetic pool of asparagus requires the development of a reliable micropropagation method. A new, rapid and efficient method of micropropagation for asparagus using rhizome bud explants has been developed. The rate of disinfection reached 90 %, and the system for shoot development and rooting on Asparagus Rhizome Bud Medium took place in one step. Recovery of the full plantlets ranged between 65 and 90 %. The plantlets were ready to be transplanted by 8 weeks, with a successful acclimatization of 80 % in average. The micropropagated plants were normal in phenotype, and the genetic stability was verified using molecular markers expressed sequence tags–microsatellites or simple sequence repeats and Flow Cytometry and certified as true-to-type. Applying this method, an in vitro breeder collection of “Morado de Huetor” landrace, A. officinalis, wild asparagus relatives and hybrid progenies has been established.

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Abbreviations

AFLP:

Amplified fragment length polymorphism

ANC:

Ancymidol

ARBM:

Asparagus rhizome bud medium

DAM:

Desjardins Asparagus medium

EDDHA:

Ethylenediamine-N,N′-bis(2-hydroxyphenylacetic acid)

EST:

Expressed sequence tags

FCM:

Flow cytometry

KIN:

Kinetin

MS:

Murashige and Skoog medium (1962)

NAA:

1-Naphthalene-acetic acid

RAPD:

Random amplified polymorphic DNA

SSRs:

Microsatellites or simple sequence repeats

2iP:

2-Isopentenil adenine

BA:

6-Benziladenine

IBA:

Indole-3-butyric acid

IAA:

Indole-3-acetic acid

bp:

Base pairs

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Authors and Affiliations

  1. Instituto de Horticultura Subtropical y Mediterránea “La Mayora”, CSIC-UMA, 29750, Algarrobo-Costa, Málaga, Spain

    E. Carmona-Martín, J. J. Regalado, I. M. G. Padilla, N. Westendorp & C. L. Encina

  2. Centro IFAPA-Churriana, 29140, Churriana, Málaga, Spain

    I. M. G. Padilla

Authors
  1. E. Carmona-Martín
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  2. J. J. Regalado
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  3. I. M. G. Padilla
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  4. N. Westendorp
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  5. C. L. Encina
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Corresponding author

Correspondence to I. M. G. Padilla.

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Carmona-Martín, E., Regalado, J.J., Padilla, I.M.G. et al. A new and efficient micropropagation method and its breeding applications in Asparagus genera. Plant Cell Tiss Organ Cult 119, 479–488 (2014). https://doi.org/10.1007/s11240-014-0548-7

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  • DOI: https://doi.org/10.1007/s11240-014-0548-7

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