Abstract
This study correlates the potential for somatic embryogenesis in cultured leaf explants of oil palm trees with changes in the expression of hormone-responsive genes. The results show that expression of the putative Aux/IAA gene EgIAA9 is specifically correlated to somatic embryogenesis. A regression model for embryogenesis indicated a predictive R2 value of 63.55 % for this expression marker. Auxin is the plant growth regulator used for induction during the early stages of oil palm tissue culture. Expression of EgIAA9 decreased in response to increasing concentrations of exogenous 2,4-D. EgIAA9 expression profiles in cultured leaf explants from highly embryogenic palms, in embryogenic and non-embryogenic calli, and in response to increasing exogenous 2,4-D, suggested that endogenous auxin levels may be greater in highly embryogenic palms. The use of this marker to predict embryogenesis in oil palm tissue culture prior to the visible onset of this event is promising. Moreover, the results of this study prompt further investigations into the role of Aux/IAA genes in oil palm somatic embryogenesis.
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Acknowledgments
We are grateful to the Director-General of the Malaysian Palm Oil Board for permission to publish this study. We are thankful to the late Madam Ho Yuk Wah from Advanced Agriecological Research Sdn. Bhd. for her invaluable insights into the selection of materials and design of this study. Our utmost appreciation also goes to Mr. Ahmad Alwi for advice on the statistical analyses. We appreciate Dr. Leow Soon Sen and Dr. Teh Ooi Kock for critical reading of the manuscript. We also thank Madam Feshah Ishak and Mr. Roslan Ngadio for technical support. This project was funded by Agribiotechnology Institute Funds, ABI(P)-1, and eScience grant 02-03-11-SF0012 from the Malaysian government.
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Ooi, SE., Choo, CN., Ishak, Z. et al. A candidate auxin-responsive expression marker gene, EgIAA9, for somatic embryogenesis in oil palm (Elaeis guineensis Jacq.). Plant Cell Tiss Organ Cult 110, 201–212 (2012). https://doi.org/10.1007/s11240-012-0143-8
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DOI: https://doi.org/10.1007/s11240-012-0143-8