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Somatic embryogenesis and shoot organogenesis from leaf explants of Primulina tabacum

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Abstracts

Primulina tabacum is a rare and endangered species that is endemic to China. Establishing an efficient regeneration system is necessary for its conservation and reintroduction. In this study, when leaf explants collected from plants grown in four ecotypes in China are incubated on Murashige and Skoog (MS) medium containing 5.0 μM thidiazuron (TDZ) for 30 days, then transferred to medium containing 5.0 μM 6-benzyladenine (BA), adventitious shoots are then observed. Conversely, when leaf explants are incubated on medium containing 5.0 μM BA for 30 days, then transferred to medium containing 5.0 μM TDZ, somatic embryogenesis is induced. This indicates that somatic embryogenesis and shoot organogenesis could be switched simply by changing the order of two cytokinins supplemented in the culture medium. Histological investigation has revealed that embryogenic cells are induced within 30 days following incubation of explants in medium containing TDZ. Only if embryogenic cells were induced, TDZ could enhance somatic embryogenesis and BA could stimulate shoot organogenesis. When comparing explants from different ecotypes, leaf explants from Zixiadong in Hunan Province could induce low numbers (1–2) of either somatic embryos or adventitious shoots on medium containing either 5.0 μM TDZ or 5.0 μM BA, respectively. Whereas, leaf explants from plants collected from the other three ecological habitats could induce 50–70 somatic embryos/adventitious shoots per explant. Moreover, somatic embryos could induce secondary somatic embryogenesis and adventitious shoots on different media. All regenerated shoots developed adventitious roots when these are transferred to rooting medium, and over 95% of plantlets have survived following acclimatization and transfer to a potting mixture (1:1, sand:vermiculite).

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Abbreviations

BA:

6-benzyladenine

TDZ:

Thidiazuron

NAA:

α-naphthalene acetic acid

KIN:

Kinetin

IBA:

3-indolebutyric acid

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Acknowledgments

Supports from the Corporation Program of National Basic Research Program of China (973 Program) (2007CB411600), Guangdong Scientific Program (2007A060306011) and the National Natural Science Foundation of China (30671711; 30972295) are gratefully acknowledged.

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Authors and Affiliations

  1. Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, The Chinese Academy of Sciences, Guangzhou, 510650, People’s Republic of China

    Xingyu Yang, Jinfeng Lü & Guohua Ma

  2. Graduate University of Chinese Academy of Sciences, Beijing, 100049, People’s Republic of China

    Xingyu Yang & Jinfeng Lü

  3. Faculty of Agriculture and Graduate School of Agriculture, Kagawa University, Miki-cho, Ikenobe 2393, Kagawa-ken, 761-0795, Japan

    Jaime A. Teixeira da Silva

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  1. Xingyu Yang
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  2. Jinfeng Lü
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  3. Jaime A. Teixeira da Silva
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  4. Guohua Ma
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Correspondence to Guohua Ma.

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Yang, X., Lü, J., da Silva, J.A.T. et al. Somatic embryogenesis and shoot organogenesis from leaf explants of Primulina tabacum . Plant Cell Tiss Organ Cult 109, 213–221 (2012). https://doi.org/10.1007/s11240-011-0087-4

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  • DOI: https://doi.org/10.1007/s11240-011-0087-4

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